First molecular evidence of the simultaneous human infection with two species of Echinococcus granulosus sensu lato: Echinococcus granulosus sensu stricto and Echinococcus canadensis

Oudni-M’rad, Myriam; M’rad, Selim; Ksia, Amine; Lamiri, Rachida; Mekki, Mongi; Nouri, Abdellatif; Mezhoud, Habib; Babba, Hamouda

doi:10.1007/s00436-015-4836-x

Cited by 12 publications

(5 citation statements)

References 27 publications

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“…3. Global distribution of the genotypes of Echinococcus canadensis and Echinococcus granulosus sensu stricto, based on molecular identification including areas of confirmed sympatry for E. ortleppi (Alam-Eldin et al, 2015;Alvarez Rojas et al, 2014;Cardona and Carmena, 2013;Carmera and Cardona, 2014;Casulli et al, 2010;Dybicz et al, 2013Dybicz et al, , 2015Farhadi et al, 2015;Guarnera et al, 2004;Hailemariam et al, 2012;Hüttner et al, 2009;Ito et al, 2013Ito et al, , 2014Jabbar et al, 2011;Jenkins et al, 2011Jenkins et al, , 2013Kędra et al, 1999Kędra et al, , 2000Konyaev et al, 2013;Lavikainen, 2005;Lavikainen et al, 2003Lavikainen et al, , 2006Lymbery et al, 2015;Mbaya et al, 2014;Mogoye et al, 2013;Moks et al, 2006;Monteiro et al, 2014;Moro et al, 2009;Nakao et al, 2007Nakao et al, , 2013bNakao et al, , 2015Oksanen and Lavikainen, 2015;Omer et al, 2010;Oudni-M'rad et al, 2016;Rinaldi et al, 2014;Rodriguez-Prado et al, 2014;Romig et al, 2015;Saarma et al, 2009;Schneider et al, 2010;Schurer et al, 2013Schurer et al, , 2014…”

Section: Pathology and Epidemiology Of Ce Highlight The Need For A DImentioning

confidence: 99%

Cystic echinococcosis: Future perspectives of molecular epidemiology

et al. 2017

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Human cystic echinococcosis (CE) has been considered to be caused predominantly by Echinococcus granulosus sensu stricto (the dog-sheep strain). Molecular approaches on CE, however, have revealed that human cases are also commonly caused by another species, Echinococcus canadensis. All indices for classification and standardization of CE pathology including available images, epidemiology, diagnostics and treatment are currently based largely on a mixture of infections which include at least E. granulosus s.s. and E. canadensis. Involvement of other species of Echinococcus in CE including E. ortleppi or otherwise cryptic diversity demonstrated recently in Africa requires further elucidation. Molecular identification of the causative species in CE cases is essential for better understanding of pathogenesis and disease. This article stresses the importance of molecular species identification of human CE as a foundation for re-evaluation of evidence-based epidemiology.

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Section: Pathology and Epidemiology Of Ce Highlight The Need For A DImentioning

confidence: 99%

Cystic echinococcosis: Future perspectives of molecular epidemiology

et al. 2017

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“…Cystic echinococcosis (CE) is a parasitic zoonosis caused by the metacestode of Echinococcus granulosus sensu lato, which has recently been classified into at least 5 species based on their genetic variation [1–4]. Intermediate hosts (livestock and humans) are infected through the oral ingestion of eggs derived from feces of the definitive host, while the protoscoleces (PSCs) develop and grow into adult worms in the intestine of dogs/foxes once the liver or lungs of the intermediate hosts containing echinococcal cysts are uptaken by the definitive hosts [5–7].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China

et al. 2019

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Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in humans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.

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“…In this study, we analysed only one CE cyst per animal/ human; consequently, mixed infections with different genotypes/haplotypes cannot be excluded. Mixed infection in a single intermediate host, including humans, has been previously reported, suggesting the outcross breeding of different adult worms in sympatric populations [25,33,[40][41][42]. Nevertheless, the concurrent infection could also be the result of a single or successive infection event in the intermediate hosts [43].…”

Section: Discussionmentioning

confidence: 95%

Genetic diversity of Echinococcus granulosus sensu lato from animals and humans in Bosnia and Herzegovina

et al. 2022

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Background Cystic echinococcosis (CE) is recognized as one of the most prevalent zoonotic diseases in Bosnia and Herzegovina. However, no systemic investigation of the genetic diversity of Echinococcus granulosus sensu lato circulating among animals and humans in the country has been performed to date. Methods In this preliminary study, we analysed one cyst each from 36 sheep, 27 cattle, 27 pigs, 11 wild boars and 16 human patients for amplification and partial sequencing of the adenosine triphosphate 6 (atp6) and cytochrome c oxidase 1 (cox1) genes. The host species, fertility rate and organ cyst location were recorded for each subject involved in the study. Results Overall, the atp6 gene was successfully amplified and sequenced from 110 samples, while 96 of the PCRs for cox1 were positive. Three zoonotic genotypes of E. granulosus sensu stricto (G1 and G3) and Echinococcus canadensis (G7) were identified in our isolates based on analyses of the atp6 gene. These genotypes were represented by 11 different genetic variants (haplotypes), six of which were identified for the first time in the present study. Conclusions This study demonstrates, for the first time, that CE in Bosnia and Herzegovina is predominantly caused by E. granulosus sensu stricto and E. canadensis clusters, which exhibited a lower genetic diversity compared to isolates from other European countries. Further molecular studies employing other mitochondrial and nuclear genes are required to better understand the transmission cycles of E. granulosus sensu stricto among intermediate and definitive hosts in the country. Graphical Abstract

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First molecular evidence of the simultaneous human infection with two species of Echinococcus granulosus sensu lato: Echinococcus granulosus sensu stricto and Echinococcus canadensis

Cited by 12 publications

References 27 publications

Cystic echinococcosis: Future perspectives of molecular epidemiology

Cystic echinococcosis: Future perspectives of molecular epidemiology

Prevalence and Molecular Characterization of Echinococcus granulosus Sensu Stricto in Northern Xinjiang, China

Genetic diversity of Echinococcus granulosus sensu lato from animals and humans in Bosnia and Herzegovina

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