First molecular evidence of
            <i>Mus musculus bactrianus</i>
            in Nepal inferred from the mitochondrial DNA
            <i>cytochrome B</i>
            gene sequences

Adhikari, Pradeep; Han, Sang‐Hyun; Kim, Yoo-Kyung; Kim, Tae-Wook; Thapa, Tej Bahadur; Subedi, Naresh; Adhikari, Prabhat; Oh, Hong-Shik

doi:10.1080/24701394.2017.1320994

Cited by 9 publications

(16 citation statements)

References 29 publications

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“…However, our study did not genotype R. rattus and Mus musculus mice. Nevertheless, earlier studies reported the presence of both R. norvegicus and M. musculus doemesticus from Bangladesh (Adhikari et al 2018 ; Barman et al 2020 ). The prevalence of RVA in our studied rodents was higher than in previous studies (Li et al 2016 ; Ianiro et al 2017 ), but in the case of shrews, our results are similar to those of a former study from China that found a 6.7% seroprevalence in wild tree shrews ( Tupaia belangeri Chinese) using antigen-capture ELISA (Wang et al 2011 ).…”

Section: Discussionmentioning

confidence: 97%

Epidemiology of Group A rotavirus in rodents and shrews in Bangladesh

et al. 2022

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Rodents and shrews live in close proximity to humans and have been identified as important hosts of zoonotic pathogens. This study aimed to detect Group A rotavirus (RVA) and its potential risk factors in rodents and shrews in Bangladesh. We captured 417 small mammals from 10 districts with a high degree of contact between people and domestic animals and collected rectal swab samples between June 2011 and October 2013. We tested the swab samples for RVA RNA, targeting the NSP3 gene segment using real-time reverse transcription-polymerase chain reaction (rRT-PCR). Overall, RVA prevalence was the same (6.7%) in both rodents and shrews. We detected RVA RNA in 5.3% of Bandicota bengalensis (4/76; 95% CI: 1.4–12.9), 5.1% of B. indica (4/79; 95% CI: 1.4–12.4), 18.2% of Mus musculus (4/22; 95% CI: 5.2–40.3), 6.7% of Rattus rattus (6/90; 95% CI: 2.5–13.9), and 6.7% of Suncus murinus (10/150; 95% CI: 3.2–11.9). We found significantly more RVA in males (10.4%; OR: 3.4; P = 0.007), animals with a poor body condition score (13.9%; OR: 2.7; P = 0.05), during wet season (8.3%; OR: 4.1; P = 0.032), and in urban land gradients (10.04%; OR: 2.9; P = 0.056). These findings form a basis for understanding the prevalence of rotaviruses circulating among rodents and shrews in this region. We recommend additional molecular studies to ascertain the genotype and zoonotic potential of RVA circulating in rodents and shrews in Bangladesh.

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Section: Discussionmentioning

confidence: 97%

Epidemiology of Group A rotavirus in rodents and shrews in Bangladesh

et al. 2022

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“…MtDNA CytB was amplified using primers L14724 and H15915 designed by Irwin et al (1991). Polymerase chain reactions (PCRs) were performed according to the procedure of Adhikari et al (2017). The purified PCR products amplified were directly sequenced with a DNA sequencing ABI 3130XL Genetic Analyzer (Applied Biosystems, Foster, CA).…”

Section: Methodsmentioning

confidence: 99%

New record of the Oriental house rat,Rattus tanezumi, in Nepal inferred from mitochondrialCytochrome Bgene sequences

Adhikari

Han

Kim

et al. 2018

Mitochondrial DNA Part B

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This study determines the presence of R. tanezumi from in Nepal using morphological and molecular analyses. Morphologically, it is indistinguishable with R. rattus owing to similar fur colour and morphometric data. However, molecular identification and phylogenetic analysis using sequences of the mitochondrial DNA (mtDNA) Cytochrome B (CytB) gene revealed two different species R. rattus and R. tanezumi from collected specimens. The genetic distance between R. rattus and R. tanezumi was found 0.043. In phylogenetic tree, the clade of R. tanezumi is distinguished into two sub-clades, R. tanezumi found in Nepal, and East Asian countries, China, Laos, Thailand, Viet Nam, and South Korea have genetic distance 0.031, suggesting the different lineages of R. tanezumi. This study confirmed the R. tanezumi present in Nepal. Our findings suggest that morphological analysis and molecular study should be carried out simultaneously for accurate identification of small sized cryptic mammals like R. tanezumi and R. rattus.

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“…Beyond these three primary subspecies, a number of secondary house mouse subspecies have also been suggested on the basis of distinct morphology 8–10 and surveys of limited numbers of molecular markers 5,10–15 . For example, mice from Yemen and Madagascar have been assigned to M. m. gentilulus due to their small size and distinct mitochondrial lineage 11,13 .…”

Section: Introductionmentioning

confidence: 99%

“…Mice from Heligoland, a small German archipelago island in the North Sea, have been characterized as M. m. helgolandicus due to their unique skull morphology, distinct mitochondrial D-loop haplotype, and allelic variation at four nuclear loci 10,16 . Similarly, mitochondrial sequence analysis has been used to assign house mice from Pakistan to the subspecies M. m. bactrianus 15,17 . M. m. musculus and M. m. castaneus naturally hybridize where their ranges overlap in Japan, and these hybrids have been designated as a distinct subspecies, M. m. molossinus 18 .…”

Section: Introductionmentioning

confidence: 99%

Taxonomic assessment of two wild house mouse subspecies using whole-genome sequencing

Lawal

Mathis

Barter

et al. 2022

Preprint

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House mice (Mus musculus) are comprised of three primary subspecies. A large number of secondary subspecies have also been suggested on the basis of divergent morphology and molecular variation at limited numbers of markers. While the phylogenetic relationships among the primary M. musculus subspecies are well-defined, the relationships among the secondary subspecies, and their relationships to primary subspecies, remain poorly understood. Here, we integrate de novo genome sequencing of museum-stored specimens of house mice from one secondary subspecies (M. m. bactrianus) and publicly available genome sequences of house mice previously characterized as M. m. helgolandicus, with whole-genome sequences from diverse representatives of the three primary house mouse subspecies. We show that mice assigned to the secondary M. m. bactrianus and M. m. helgolandicus subspecies are not genetically differentiated from the contemporary M. m. castaneus and M. m. domesticus, respectively. Overall, our work suggests that the M. m. bactrianus and M. m. helgolandicus subspecies are not well-justified taxonomic entities, emphasizing the importance of leveraging whole-genome sequence data to inform subspecies designations. Additionally, our investigation has established experimental procedures for generating whole-genome sequences from air-dried museum specimens and provides key genomic resources to inform future genomic investigations of wild mouse diversity.

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First molecular evidence of Mus musculus bactrianus in Nepal inferred from the mitochondrial DNA cytochrome B gene sequences

Cited by 9 publications

References 29 publications

Epidemiology of Group A rotavirus in rodents and shrews in Bangladesh

Epidemiology of Group A rotavirus in rodents and shrews in Bangladesh

New record of the Oriental house rat,Rattus tanezumi, in Nepal inferred from mitochondrialCytochrome Bgene sequences

Taxonomic assessment of two wild house mouse subspecies using whole-genome sequencing

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