First Molecular Evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in Dogs from Kyrgyzstan

Abstract

Tick-borne parasitic diseases cause mild to severe infections among vertebrate hosts, including dogs. Species in the genus Babesia are important tick-borne pathogens and have worldwide distributions. Although there are data on the prevalence and distribution of Babesia species among dogs around the world, there is no information available in Kyrgyzstan, according to a literature review. In this study, 337 dogs were screened by nested PCR for the presence of the 18S small subunit ribosomal RNA (18S SSU rRNA) gene of piroplasm species. Overall prevalence was 6.23% (21/337) for Babesia/Theileria spp. DNA sequencing of positively tested samples revealed that eighteen samples were infected with Babesia vogeli (B. vogeli) (5.34%), two samples with B. vulpes (0.59%), and one sample with Theileria ovis (T. ovis) (0.29%). The phylogenetic analyses and nucleotide sequences in contrast with those present in GenBank revealed that two nucleotide substitutions (594th and 627th) were found between B. vogeli isolates, including ours, indicating that the mutation is relatively rare. The sequences of other pathogens obtained in this study confirmed 100% nucleotide identity with B. vulpes and T. ovis sequences in GenBank. To the best of our knowledge, B. vogeli, B. vulpes, and T. ovis were detected for the first time in dogs from Kyrgyzstan, and it is thought that results will contribute to the understanding of the epidemiology of canine tick-borne pathogens in the country.

Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi

“…In recent years, these methods have been widely used in epidemiological studies (46)(47)(48)(49)(50). Although the presence of different TBPs in cattle, sheep, and dogs (HM species, B. vogeli, B. vulpes, A. centrale, A. capra, A. phagocytophilum-like 1, A. ovis, B. major, T. annulata, and T. orientalis) has been previously reported in Kyrgyzstan (14,15,(31)(32)(33)(34)(35)(36), this study is the first molecular study investigating TBPs in horses in the country.…”

“…The PCR assays were performed as described before ( 19 , 20 , 38–41 ), and the genomic DNA of B. vogeli (Accession number: OR116199) ( 33 ), A. phagocytophilum (Accession number: OP828919) ( 42 ), A. capra (Accession number: ON783818) ( 22 ), and M. wenyonii (Accession number: OM468183) ( 43 ) were used as the positive controls, and DNase-RNase-free sterile water (Cat No. : 129114, Qiagen ® , Germany) was used as the negative control in the PCRs.…”

“…To date, no record of equine-TBPs has been found in the literature in Kyrgyzstan. Recently, it has been reported first molecular presence and prevalence of the important TBPs such as canine and bovine hemotropic mycoplasma species ( 31 , 32 ), B. vogeli, B. vulpes in dogs ( 33 ), A. centrale, A. capra , A. phagocytophilum -like 1, B. major, T. annulata, and T. orientalis in cattle ( 14 , 15 , 34 , 35 ), A. ovis, A. capra , and A. phagocytophilum -like 1 in sheep ( 36 ) in Kyrgyzstan. In this study, we aimed to survey of TBPs (EP, HM, Anaplasma phagocytophilum, and Anaplasma capra ) in grazing horses in Kyrgyzstan and to detect the genetic diversity of the pathogens.…”

Multiplex primers employment for detection of rinderpest virus RNA by PCR

“…K. Altay et al (2023) and R.M. Nayak et al (2020), focused on innovative methods, and support efforts in the use of multiplex primers.…”

“…In some cases, complications may occur in the form of pneumonia, which can be fatal, or paralysis. K. Altay et al (2023) and K. Beus et al (2023) state that accurate detection of the disease plays an important role in controlling and preventing the spread of the disease. The most effective method for diagnosing rinderpest today is polymerase chain reaction (PCR).…”