First molecular detection of <i>Nosema ceranae</i> in Azerbaijan

Ütük, Armağan Erdem; Aliyeva, Rafiga; Girişgin, Oya; Gökmen, Tülin; Özüiçli, Mehmet; Aydın, Levent

doi:10.1080/00218839.2019.1614737

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Nosemosis is an extremely important and common honeybee disease caused by Nosema apis and Nosema ceranae (3,4). As in the rest of the world, Nosema disease has a wide range of distribution in Turkey, and it is speculated to cause major losses from time to time.…”

Section: Discussionmentioning

confidence: 99%

“…TTATTTTGAGAGAACGGTTTTTTGTttgAGAATGACCGGGA of the disease in content. In the last few years, however, determination of the disease at species level and speciesspecific molecular biological studies have been carried out (3,4,34,35). Ütük et al (3) detected N. ceranae for the first time in two bee samples that were sent to their laboratory from the Samsun and Giresun provinces of Turkey by using multiplex PCR.…”

Section: Gcaagattgaaaattaaagaaattgacggaagaataccacaaggagtg Gattgtgcggcttaatttgactcaacgcgaggtaacttaccaatattttmentioning

confidence: 99%

“…In bees, many diseases are observed caused by bacteria, viruses, parasites, and fungi. One of the bee parasites, Nosema, is an extremely important and common honeybee disease caused by microsporidia Nosema apis and Nosema ceranae, which are quite effective in adult honeybees (3,4). Although the life cycles of these species are similar, in the studies carried out, N. ceranae has been reported to be more pathogenic, or in other words to have more damaging features (3,5).…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Oğuz¹,

Karapınar²,

Dinçer³

et al. 2017

Turk J Vet Anim Sci

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This study was planned to determine the prevalences of the Nosema spp. and the black queen-cell virus (BQCV) among honeybees (Apis mellifera) raised in the province of Van by PCR and to determine the molecular characteristics of the determined isolates. A total of 260 adult worker bees from 26 colonies at 5 apiary locations belonging to the province of Van in April and May 2015 were collected for this reason. Samples were examined microscopically. In the case of positivity, spore identification was done by multiplex PCR. Reverse transcription/PCR analysis (RT/PCR) was carried out for the BQCV analysis. At the end of the microscopic examination, Nosema spp. spores were detected in 8 out of 26 colonies (32.5%). The result of multiplex-PCR revealed Nosema ceranae positivity in all of the samples, but no Nosema apis was determined. As a result of the RT/PCR tests of the samples BQCV was detected in 23 (88.5%) of the total 26 colonies. This study is the first to investigate Nosema spp. and BQCV with the PCR technique in bees raised in the province of Van.

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Section: Discussionmentioning

confidence: 99%

Section: Gcaagattgaaaattaaagaaattgacggaagaataccacaaggagtg Gattgtgcggcttaatttgactcaacgcgaggtaacttaccaatattttmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Oğuz¹,

Karapınar²,

Dinçer³

et al. 2017

Turk J Vet Anim Sci

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“…Apis D,N X [16,18,28,29,30,31,32,33,34,35,36,37,38,39,40,41] (5) X [43] (>1) X [4,13,16,18,29,30,31,32,33,35,36,37,40,41,43,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67] (>10)…”

Section: Host Genera Nosema Apis Nosema Bombi Nosema Ceranaementioning

confidence: 99%

A growing pandemic: A review of Nosema parasites in globally distributed domesticated and native bees

Grupe

Quandt

2020

PLoS Pathog

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Nosema infection in bees Domesticated and native bees face a variety of deadly threats that cause mortality and reduced fecundity and thus, by extension, endanger agriculture and native plant communities that rely on bees for pollination. Biotic factors negatively impacting bees include: viruses, nematodes, mites, bacteria, and fungi. Additionally, abiotic threats include the destruction of nesting and floral resources from anthropogenic sources as well as a plethora of negative factors from climate change. While a substantial amount of research has been done investigating the causes of colony collapse disorder in the European honey bee, Apis mellifera, there is growing evidence over the past two decades that another pandemic of bees, both domesticated and native, is growing. This pandemic is the result of the spread of fungal pathogens in the genus Nosema. Nosema species belong to Microsporidia, which are all unicellular, obligate symbionts of animals, and gregarines. Although long thought to be protists, Microsporidia are now recognized as a highly reduced lineage of fungi [1]. Tokarev and colleagues [2] recently placed Nosema species that infect bees (Anthophila, Hymenoptera) within a new genus, Vairimorpha, but for the sake of consistency with the existing literature this Review article will refer to them simply as Nosema. Specifically, Nosema carry out their life cycle by infecting the cells in the midgut of bees. Once a spore is ingested by a bee and reaches the midgut, it will germinate. It then injects its contents into the host cell where it consumes the cell contents via phagocytosis until it eventually lays down spore walls before rupturing the host cell to release the spores [3]. These spores can then infect other cells in the digestive tract or be passed out of the host in excrement, thereby contaminating floral resources, collected pollen, and the nesting environment. Other bees are then susceptible to ingest spores in the nest via fecal-oral transmission, or if excreted at a floral resource, the fungus can infect any susceptible hosts that come into contact with that flower [4,5]. Due to the extent of bee foraging ranges, this process not only increases the local pathogen load but also serves to disperse Nosema to new habitats and novel hosts. In addition to the natural transmission of these pathogens, commercial products such as honey, bee pollen, and royal jelly can be contaminated and potentially disperse these pathogens [6]. The most common symptoms of Nosema infection are dysentery and microscopic lesions within the gut and Malpighian tubules. This leads to host frailty, lethargy, and loss of workers in eusocial bees that reduces foraging ability for the colony through mortality, reduced homing ability, shorter foraging flights, and inefficient foraging behavior [5,7]. Nosema bombi infections also reduce the fecundity of the colony through detrimental physical effects to the reproductive organs in male bumblebees, increased mortality of workers, and negatively impacting

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“…From 2015 till to now the entire world suffers from this Epidemic disease. The first detection of N. ceranae is variable among the countries such Turkey in 2010 [20], Iran in 2011 [21], Jordan in 2014 [22] Saudi Arabia in 2016 [23], Azerbaijan in 2016 [24] Recently in 2020, the first detection of N. ceranae was reported in the northern region of Baghdad (Capital of Iraq ) [25]. Sulaimaniyah city is consider one of the leading cities in Iraq for honey production, however, the consequences of several honey bee diseases decreased the level of honey production and economically affected the apiculture [7,8].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2023

SJCUS

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First molecular detection of Nosema ceranae in Azerbaijan

Cited by 6 publications

References 14 publications

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

A growing pandemic: A review of Nosema parasites in globally distributed domesticated and native bees

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