First molecular detection and characterization of tick-borne pathogens in water buffaloes in Bohol, Philippines

Galon, Eloiza May; Moumouni, Paul Franck Adjou; Ybañez, Rochelle Haidee D.; Ringo, Aaron Edmond; Efstratiou, Artemis; Lee, Seunghun; Liu, Mingming; Guo, Huanping; Gao, Yang; Li, Jixu; Salces, Caro B.; Maurillo, Bon Christian A.; Boldbaatar, Damdinsuren; Ybañez, Adrian P.; Xu, Xuan

doi:10.1016/j.ttbdis.2019.03.016

Cited by 12 publications

(11 citation statements)

References 43 publications

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“…In the present study, PCR amplification with the A. marginale species-specific primers showed that the majority of detected rickettsial organisms were A. marginale (74.87% of all rickettsial pathogens-positive samples), which was in agreement with previous reports from Thailand [ 11 ] and the Philippines [ 22 ]. However, it is important to note that only 37 of these ‘ A. marginale’ amplified sequences were confirmed for species designation (i.e.…”

Section: Discussionsupporting

confidence: 93%

“…This high prevalence of buffalo positive for infection with rickettsial organisms was similar to those observed previously in Cuba (52% [20]) and Mozambique (72.2% [21]), but was higher than those reported in the Philippines (29% [22]), Malaysia (21.8% [23]), India (18.33% [24]), South Africa (17.3% [25]), Pakistan (14.73% [17]), Columbia (13.1% [26]), and northeast Thailand (8% [11]). In this study, the highest anaplasmosis prevalence was found in Southern Thailand (Phatthalung), while the lowest was at Northeastern Thailand (Nong Bua Lamphu).…”

Section: Discussionsupporting

confidence: 89%

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Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

2020

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Background Anaplasmosis, an animal disease caused by rickettsial bacteria in the genus Anaplasma, is of considerable economic importance in livestock animals in many countries worldwide. The objectives of this study were to determine the identity, prevalence, and geographic distribution of Ehrlichia and Anaplasma in naturally infected water buffalo in Thailand using PCR amplification and sequencing of the 16S ribosomal RNA and heat shock protein groEL genes. A total of 456 buffalo blood samples from Thailand were investigated. Species identification and genetic differentiation of intra-population and inter-population with the global isolates were conducted based on nucleotide sequences. Interplay between the infection and host factors was also assessed. Results Overall, 41% of water buffalo were found to be infected with rickettsial organisms in the family Anaplasmataceae, but Ehrlichia spp., Neorickettsia spp., and Wolbachia spp. were not found in any of the sequenced samples in this study. Female buffalo were more frequently infected with bacteria in the family Anaplasmataceae than males [71 out of 176 females (40.3%) versus 11 out of 47 males (23.4%)]. The Odds Ratio value indicated that the risk of infection for female buffalo was 2.2-fold higher than that for males (p < 0.05). We detected three haplotypes of A. marginale 16S rRNA gene and they were placed in a clade that was closely related to the A. marginale in buffalo in China; and cattle in Thailand, Uganda, and China. Homology searching of groEL sequences against the GenBank™ database using the BLASTn algorithm revealed that the obtained sequences had a high percentage similarity (98.36–99.62%) to A. platys sequences. The groEL sequences of three A. platys-like isolates were clustered in the same clade as the A. platys from the tick Rhipicephalus microplus in China. Conclusions Our data showed that the apparently healthy buffalo were naturally infected by bacteria in the family Anaplasmataceae at a relatively high prevalence. We also report the finding of A. platys-like infections in water buffalo in Thailand for the first time. Water buffalo serving as the reservoir host of anaplasmosis is of concern for managing the disease control and prevention in ruminants.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 89%

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

2020

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“…Furthermore, A. ovis (79.0%), A. bovis (24.7%), and A. phagocytophilum (28.4%) in Yecheng sheep in current study, while A. ovis (1.5%), A. marginale (5.7%), and A. centrale (2.7%) were identified in ticks from Pakistan, the country that Yecheng borders (25). Other border countries of XUAR also have reports about Anaplasma infection (A. bovis, A. centrale, A. phagocytophilum, and A. marginale) in ruminants or ticks including Russia (26), Philippines (27), and south India (28). Notably, A. capra, a newly discovered emerging zoonotic species of Anaplasma, was not identified in the current study, although it has been reported in both XUAR (only four positive samples) and Gansu of China (29,30).…”

Section: Resultssupporting

confidence: 43%

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Galon

Guo

et al. 2020

Front. Vet. Sci.

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Babesia, Theileria, and Anaplasma are important causative agents of tick-borne diseases that severely affect sheep. However, there is paucity in the occurrence genetic diversity of the infections of tick-borne diseases in sheep in border regions, northwestern China. In this study, nested polymerase chain reaction (nPCR) assays and gene sequencing were used to identify tick-borne Babesia spp., Theileria spp., and Anaplasma spp. infections in border regions, northwestern China. Out of 323 samples tested in this study, 225 (69.7%) sheep were infected with Babesia spp., Theileria spp., and Anaplasma spp. Two hundred six (63.8%), 60 (18.6%), 54 (16.7%), 51 (15.8%), 32 (9.9%), 19 (5.9%), and 16 (5.0%) were positive for A. ovis, B. motasi-like, A. bovis, T. uilenbergi, A. phagocytophilum, T. luwenshuni, and B. motasi-like Xinjiang, respectively. The most common dual infection was with A. ovis and B. motasi-like while the most frequent triple coinfection was A. ovis, B. motasi-like, and T. uilenbergi with coinfection rates of 17.0% (55/323) and 5.0% (16/323), respectively. Sequencing analysis indicated that A. ovis MSP4, A. phagocytophilum epank1, A. bovis 16S rRNA, B. motasi-like rap1-b, B. motasi-like Xinjiang rap1-a, T. luwenshuni 18S rRNA, and T. uilenbergi 18S rRNA from border regions, northwestern China, showed 99-100% identity with documented isolates from other countries. To the best of the authors' knowledge, this is the first report of T. uilenbergi and T. luwenshuni infections of sheep in border regions, northwestern China. Furthermore, these findings provide important data for understanding the distribution of Babesia, Theileria, and Anaplasma in sheep between border countries and China.

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“…Based on statistical analysis, A. marginale infection ( P =0.0001) was significantly higher in cattle, while hemoplasma infection ( P =0.011) was significantly higher in water buffaloes ( Table 1 ). Although both ruminants are natural hosts, clinical anaplasmosis affects cattle more frequently, while water buffaloes act as reservoir hosts [ 12 , 14 ]. On the other hand, there is little information on the susceptibility of water buffaloes to hemoplasma.…”

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confidence: 99%

Molecular survey of tick-borne pathogens infecting backyard cattle and water buffaloes in Quezon province, Philippines

et al. 2020

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Tick-borne diseases (TBD) cause enormous losses for farmers. Backyard raising comprises majority of the livestock population in the Philippines, but TBD information in backyard livestock is scarce. In this study, 48 cattle and 114 water buffalo samples from Quezon province, Philippines were molecularly screened for tick-borne pathogens. Anaplasma marginale (16.67%) and hemoplasma (20.99%) were detected in the samples. A. marginale infection ( P =0.0001) was significantly higher in cattle, while hemoplasma infection ( P =0.011) was significantly higher in water buffaloes. A. marginale isolates from this study were highly similar to previous isolates from the Philippines while Mycoplasma wenyonii and Candidatus Mycoplasma haemobos were the identified hemoplasma species. Our findings reveal additional information on the TBD situation of Philippine backyard livestock.

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First molecular detection and characterization of tick-borne pathogens in water buffaloes in Bohol, Philippines

Cited by 12 publications

References 43 publications

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Molecular survey of tick-borne pathogens infecting backyard cattle and water buffaloes in Quezon province, Philippines

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