First Molecular Characterization of Aspiculuris tetraptera (Nematoda: Heteroxynematidae) from Mus musculus (Rodentia: Muridae) in India

Abstract

Mus musculus (Rodentia: Muridae) were infected by a nematode belonging to the order Oxyurida. According to external morphological characters it was found to be Aspiculuris tetraptera Schulz, 1924. Present communication deals with molecular characterization of A. tetraptera infecting intestine of Mus musculus in Meerut (U.P.), India. A partial sequence of 18S ribosomal and cytochrome c oxidase subunit 1 (Cox 1) mitochondrial DNA analyses were performed. Sequence corresponding to 18S rRNA and mt Cox 1 gene was identical to sequences reported for A. tetraptera on GenBank. These results confirm the taxonomic validation of A. tetraptera parasitizing Mus musculus. This is the first report of molecular study of A. tetraptera in India.

Aspiculuris mexicana n. sp. (Nematoda: Heteroxynematidae) parasite of Cricetidae rodents from Mexico, with a taxonomic key for the species of the genus

“…not reliable. We partially agree with this statement since only 5 of the 20 species of the genus have been studied from a molecular perspective (Behnke et al, 2015;Goswami et al, 2015). In addition, the identification of Aspiculuris is not based exclusively on the number and arrangement of the caudal papillae, but on a combination of morphological characters additional to these, such as the terminal region of the cervical alae with respect to the bulb of the esophagus, size of the eggs, length of the male and female body, tail enclosed or not by caudal alae, among others (Falcón-Ordaz et al, 2010;Liu et al, 2012).…”

Procamallanus spiculogubernaculus Agarwal, 1958 (Nematoda: Camallanidae) from Stinging catfish, Heteropneustes fossilis in India: morphological characterization and molecular data

“…By comparing the sequence differences, it is easy to identify closely related nematode species. The molecular markers based on nuclear and mitochondrial genes are an excellent tool for differentiating nematode species (Floyd et al, 2002;Blaxter et al, 2005;McLean et al, 2012;Singh et al, 2013;Nakacwa et al, 2013;Chaudhary et al, 2015;Mikaeili et al, 2015;Goswami et al, 2015). The present molecular analyses using 18S rDNA and cox1 mtDNA genes support the placement of P. spiculogubernaculus in family Camallanidae.…”

“…Therefore, morphological identifi cation needs the use of molecular analyses to supplement the proper identifi ca-tion and validation of species. Molecular tools are frequently used in nematode identifi cation and systematics (Gasser, 2001;Nadler et al, 2005;Mattiucci & Nascetti, 2008;Borges et al, 2012;Jones et al, 2012;Liu et al, 2013;Pekmezci et al, 2014;Di Azevedo et al, 2015;Goswami et al, 2015Goswami et al, , 2016. In this study, supporting morphological and molecular information is provided for P. spiculogubernaculus Agarwal, 1958 from the freshwater fi sh H. fossilis collected from Ghazipur, Delhi, India.…”

Distribution extension of Aspiculuris americana parasite of Peromyscus difficilis in Hidalgo, México

“…In the second group, cervical alae gradually decrease its width, joining the wall-body or to the lateral alae if there, A. asiatica, A. africana, A. ratti, A. witenbergi, and A. shikoloveta. These species are distributed in Africa, North America, Asia and Europe, only A. tetraptera has a worldwide distribution (Arya 1980;Hugot 1980;Manna et al 1985;Liu et al 2012).…”

“…from Tepeapulco (Falcón-Ordaz et al 2015). Aspiculuris are parasites of Muroidea (Rodentia); currently includes 21 nominal species (Arya 1980;Manna et al 1985;Liu et al 2012) distributed worldwide (Goswami et al 2015). In México has been registered in Neotoma mexicana (García-Prieto et al 2012).…”