First mitochondrial genomes of five hoverfly species of the genus Eristalinus (Diptera: Syrphidae)

Abstract

The hoverfly genus Eristalinus (Diptera, Syrphidae) contains many widespread pollinators. The majority of the species of Eristalinus occur in the Afrotropics and their molecular systematics still needs to be investigated. This study presents the first complete and annotated mitochondrial genomes for five species of Eristalinus. They were obtained by high-throughput sequencing of total genomic DNA. The total length of the mitogenomes varied between 15 757 and 16 245 base pairs. Gene composition, positions, and orientation were shared across species, and were identical to those observed for other Diptera. Phylogenetic analyses (maximum likelihood and Bayesian inference) based on the 13 protein coding and both rRNA genes suggested that the subgenus Eristalinus was paraphyletic with respect to the subgenus Eristalodes. An analysis of the phylogenetic informativeness of all protein coding and rRNA genes suggested that NADH dehydrogenase subunit 5 (nad5), cytochrome c oxidase subunit 1, nad4, nad2, cytochrome b, and 16S rRNA genes are the most promising mitochondrial molecular markers to result in supported phylogenetic hypotheses of the genus. In addition to the five complete mitogenomes currently available for hoverflies, the five mitogenomes published here will be useful for broader molecular phylogenetic analyses among hoverflies.

Description of the preimaginal stages of the golden native dronefly from Australia, Eristalinus punctulatus (Macquart, 1847) (Diptera: Syrphidae)

“…All these morphological traits appear in E. punctulatus larvae. Despite there being about 75 known species of Eristalinus (Sonet et al 2019, just four species have had their larval stage described: E. sepulchralis, E. megacephalus, E. aeneus and E. taeniops (Pérez-Bañón et al 2003;Zalat & Mahmoud 2009).…”

The complete mitochondrial genome of Helophilus virgatus (Diptera: Syrphidae: Eristalinae) with a phylogenetic analysis of Syrphidae

“…So far, only 11 syrphine species' mitogenomes have been sequenced completely or partially and are available in the GenBank (https://www.ncbi.nlm.nih.gov/) (Cameron et al 2007;Tang et al 2014;Junqueira et al 2016;Li et al 2017;Pu et al 2017;Sonet et al 2019). Herein, we sequenced the twelfth mitogenome, of H. virgatus, the detailed information about this sequence and a phylogenetic tree based on dataset of all syrphine mitogenomes are given in the following text.…”

Checklist of hover flies (Diptera, Syrphidae) of the Republic of Georgia

“…The subgenus for each species, when this applies, has been indicated with the exception of the genus Eristalinus, as current subdivision of this genus based on morphological characteristics of the eyes in males (Thompson and Rotheray 1998) is not supported by molecular analyses (Pérez-Bañón et al 2003b;Sonet et al 2019).…”