First magenetic resonance imaging studies on aluminium maltolate‐treated aged New Zealand rabbits: an Alzheimer's animal model

Obulesu, Magisetty; Dowlathabad, Muralidhara Rao; Raichurkar, Keshav Prakash; Mannar, Shamasundar N.

doi:10.1111/psyg.12158

Cited by 4 publications

(1 citation statement)

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“…Another finding of our study was that JWR rabbits fed a 2% cholesterol diet showed significant cortical and hippocampal atrophy, in accordance with current clinical standards for AD [ 16 – 18 , 41 ].Similar to previous studies based on New Zealand White rabbit model of AD [ 42 ], cholesterol-fed JWR rabbits provided support for one of the most consistent findings in AD—an ventricular enlargement. In our study, the atrophy of the hippocampus is more evident than the cortex in cholesterol-fed rabbits, and the enlargement of the lateral ventricle adjacent to the hippocampus is more evident than the third ventricle.…”

Section: Discussionsupporting

confidence: 89%

Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging

Jin

Pan

et al. 2018

Lipids Health Dis

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BackgroundHypercholesterolemia is known to increase the risk of AD in later life, the purpose of this study is to illustrate brain metabolic and structural changes in a cholesterol-fed rabbit model of Alzheimer’s Disease (AD) by using clinical 3 T Magnetic Resonance Imaging (MRI).MethodsThe Institutional Animal Care and Use Committee of Zhejiang Chinese Medical University approved the study. Totally 16 Japanese White Rabbits (JWR) were randomly divided into 2 groups including normal control group fed with routine diet (group NC) and high cholesterol diet group (group CD) fed a 2% cholesterol diet with 0.24 ppm copper in the drinking water for 12 weeks. Magnetic resonance spectroscopy (MRS) and structural image of rabbit brain were performed by using a 3 Tesla (T) MRI scanner with an 8 channel Rabbit coil. The chemical metabolites were identified by LC Model including N-acetylaspartate (NAA), creatine (Cr), glutamate (Glu), glutamine (Gln), Glycerophosphatidylcholine (GPC), phosphorylcholine (PCH), and myoinositol (MI). The relative concentrations (/Cr) were analyzed. Additionally, Amyloid-β (Aβ) accumulation in the brain was measured postmortem. For comparisons of MR and Aβ data between groups, two-tailed t-tests were performed.ResultsThe ratio of NAA/Cr (0.76 ± 0.10) and Glu/Cr (0.90 ± 0.14) in group CD were lower than those in the group NC (0.87 ± 0.06, 1.13 ± 0.22, respectively, P < 0.05). Compared to the group NC (2.88 ± 0.09 cm3, 0.63 ± 0.08 cm3, respectively), the cortical and hippocampal volumes (2.60 ± 0.14 cm3 and 0.47 ± 0.07 cm3, respectively) of rabbits brain decreased in the group CD while the third and lateral ventricular volumes enlarged (44.56 ± 6.01 mm3 vs 31.40 ± 6.14 mm3, 261.40 ± 30.98 mm3 vs 153.81 ± 30.08 mm3, P < 0.05). These metabolic and structural changes were additionally accompanied by the significant increase of Aβ1–42 in the cortex and hippocampus (163.60 ± 16.26 pg/mg and 215.20 ± 69.86 pg/mg, respectively, P < 0.05).ConclusionHigh cholesterol diet can induce the brain metabolic and structural changes of the rabbit including lowered level of NAA and Glu and the atrophy of the brain which were similar to those of human AD.

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