First isolation of Tropheryma whipplei from bronchoalveolar fluid and clinical implications

Fénollar, Florence; Ponge, T.; Scola, Bernard La; Lagier, Jean-Christophe; Lefèbvre, M.; Raoult, Didier

doi:10.1016/j.jinf.2011.11.026

Cited by 36 publications

(38 citation statements)

References 14 publications

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“…Since then, several studies have been performed, and from these studies, it became clear that on average, it takes at least 30 days to detect T. whipplei in these cultures (21,25,26). This slow replication rate of T. whipplei severely impairs routine culture-based diagnostics.…”

Section: Bacteriology Culturementioning

confidence: 99%

“…This slow replication rate of T. whipplei severely impairs routine culture-based diagnostics. Culture of T. whipplei is further improved by the use of axenic medium instead of cell culture medium, and this greatly simplifies the isolation of strains from patient samples (21,25,26). In addition, disinfection of patient samples by filtration and pretreatment with glutaraldehyde greatly improves the success rate (25).…”

Section: Bacteriology Culturementioning

confidence: 99%

“…Although these spores have never been observed, they may explain the long-term survival in the environment. Finally, in addition to the oral route of transmission, there is some evidence for a respiratory route, as T. whipplei was shown to be present in bronchoalveolar lavage (BAL) fluid samples of patients with pneumonia (26,56,57).…”

Section: Human Carriage and Human-to-human Transmissionmentioning

confidence: 99%

“…There are four commonly recognized manifestations of infections with T. whipplei (Table 1) (15,16,26,100,101): (i) classic Whipple's disease; (ii) localized chronic infections, predominantly endocarditis; (iii) acute infections, e.g., pneumonia, bacteremia, and gastroenteritis; and (iv) carriage. All of these will be discussed in detail below.…”

Section: Clinical Manifestationsmentioning

confidence: 99%

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Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

et al. 2017

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SUMMARY Whipple's disease is a rare infectious disease that can be fatal if left untreated. The disease is caused by infection with Tropheryma whipplei, a bacterium that may be more common than was initially assumed. Most patients present with nonspecific symptoms, and as routine cultivation of the bacterium is not feasible, it is difficult to diagnose this infection. On the other hand, due to the generic symptoms, infection with this bacterium is actually quite often in the differential diagnosis. The gold standard for diagnosis used to be periodic acid-Schiff (PAS) staining of duodenal biopsy specimens, but PAS staining has a poor specificity and sensitivity. The development of molecular techniques has resulted in more convenient methods for detecting T. whipplei infections, and this has greatly improved the diagnosis of this often missed infection. In addition, the molecular detection of T. whipplei has resulted in an increase in knowledge about its pathogenicity, and this review gives an overview of the new insights in epidemiology, pathogenesis, clinical manifestations, diagnosis, and treatment of Tropheryma whipplei infections.

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Section: Bacteriology Culturementioning

confidence: 99%

Section: Bacteriology Culturementioning

confidence: 99%

Section: Human Carriage and Human-to-human Transmissionmentioning

confidence: 99%

Section: Clinical Manifestationsmentioning

confidence: 99%

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Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

et al. 2017

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“…The natural history of Tropheryma whipplei continues to be clarified 1,2 ; following contamination, patients develop acute infections including gastroenteritis, bacteremia, and pneumonia, [3][4][5][6][7] and they may develop specific antibodies. Depending on host factors, three methods of evolution are currently considered.…”

Section: Introductionmentioning

confidence: 99%

Looking for Tropheryma whipplei Source and Reservoir in Rural Senegal

Keïta

Mediannikov²,

Ratmanov³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract. Tropheryma whipplei, the bacterium linked to Whipple's disease, is involved in acute infections and asymptomatic carriage. In rural Senegal, the prevalence of T. whipplei is generally high but is not homogeneous throughout households in the same village. We studied environmental samples collected in two Senegalese villages and conducted the survey to investigate the difference between households. Overall, the comparison between five households with very high T. whipplei prevalence and three households without any registered cases showed that the only difference was the presence of toilets in the latter (1/5 versus 3/3; P = 0.01423). Among the 1,002 environmental specimens (including domestic and synanthropic animals and dust sampled in households) tested for T. whipplei DNA, only four specimens were slightly positive. Humans are currently the predominant identified reservoir and source of T. whipplei in these populations. Limited access to toilets and exposure to human feces facilitate the fecal-oral transmission of T. whipplei.

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Bacterial Diseases

Farver¹

2018

Pulmonary Pathology

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First isolation of Tropheryma whipplei from bronchoalveolar fluid and clinical implications

Cited by 36 publications

References 14 publications

Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

Looking for Tropheryma whipplei Source and Reservoir in Rural Senegal

Bacterial Diseases

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