First isolation and antinociceptive activity of a lipid transfer protein from noni ( Morinda citrifolia ) seeds

Campos, Dyély C.O.; Costa, Andréa Santos; Lima, Amanda Barroso; Silva, Fredy D.A.; Lobo, Marina Duarte Pinto; Monteiro-Moreira, Ana Cristina de Oliveira; Moreira, Renato A.; Leal, Luzia Kalyne Almeida Moreira; Miron, Diogo; Vasconcelos, Ilka M.; Oliveira, Hermógenes David de

doi:10.1016/j.ijbiomac.2016.01.029

Cited by 22 publications

(14 citation statements)

References 55 publications

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“…44.566) has been deposited in the Prisco Bezerra Herbarium of the Federal University of Ceará for future reference. The lipid transfer protein McLTP 1 was purified from defatted noni seed flour, as previously described by Campos et al (2016). The purity of McLTP 1 was assessed by non-reducing 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; Laemmli 1970).…”

Section: Plant Materials and Purification Of Mcltpmentioning

confidence: 99%

“…Morinda citrifolia L., Rubiaceae, is a plant species native to tropical Asia, whose roots, bark, and fruits have been used for the treatment of several diseases, such as diabetes, rheumatoid arthritis, hypertension, inflammation, and pain management (Torres et al 2017). Recently, Campos et al (2016) isolated a thermostable lipid transfer protein (LTP) from M. citrifolia seeds, named McLTP 1 (UniProt KB accession number: C0HJH5) that exhibited anti-inflammatory and antinociceptive activities when administered orally in mice. According to Campos et al (2017), the anti-inflammatory effects of McLTP 1 involve the modulation of the levels of pro-and anti-inflammatory cytokines, such as interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α).…”

Section: Introductionmentioning

confidence: 99%

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Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Silveira

Chaves-Filho

Macêdo

et al. 2020

Rev. Bras. Farmacogn.

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Gentamicin is an aminoglycoside antibiotic used in the treatment of gram-negative bacterial infections. However, its prolonged use is limited due to a high risk of nephrotoxicity. This study aimed to investigate the protective effects of a lipid transfer protein isolated from Morinda citrifolia L., Rubiaceae, seeds against gentamicin-induced nephrotoxicity in rats, and in a proximal tubular epithelial cell line from Rhesus monkey (LLC-MK2). Treatment with 6.25-25 μg/ml of the protein protected cells against cytotoxicity induced by 2 mM gentamicin. In the in vivo study, protein treatment (8 mg/kg, orally administered daily for 7 days 2 h after intraperitoneal injection of 100 mg/kg gentamicin) improved the levels of creatinine, urea, and uric acid. Additionally, it alleviated oxidative stress and inflammation induced by gentamicin, including a reduction in myeloperoxidase activity and cytokine modulation. Data were corroborated with histological analysis showing attenuated alterations by protein treatment. These findings suggest that this protein protects against gentamicin-induced nephrotoxicity. In addition, this natural product may be as an adjunctive tool to reduce kidney toxicity and improve the clinical usage profile of gentamicin.

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Section: Plant Materials and Purification Of Mcltpmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Silveira

Chaves-Filho

Macêdo

et al. 2020

Rev. Bras. Farmacogn.

Self Cite

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“…The results of formalin test indicated that TA had central and peripheral anti-nociceptive properties. As is known to all, naloxone is a one of non-selective opioid receptor antagonists [22]. Pretreatment with naloxone (2 mg/kg) didn't reverse the antinociceptive activity of TA administered orally.…”

Section: Discussionmentioning

confidence: 89%

Anti-nociceptive effect of total alkaloids isolated from the seeds of Areca catechu L (Arecaceae) in mice

Zhao¹,

Yu²,

Yang³

et al. 2017

Trop. J. Pharm Res

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“…Finally, purification is completed by reverse-phase high-performance liquid chromatography (RP-HPLC), in which the nonpolar amino acid residues of nsLTPs adhere to the hydrophobic chain matrix (ranging from C 4 to C 18 ). These combinations ensure yields of 10 to 773 mg of nsLTPs per kilogram of seeds [ 77 , 87 , 90 , 91 ] and 4.2 mg of nsLTPs per gram of extracted proteins [ 85 ].…”

Section: Identification and Isolationmentioning

confidence: 99%

“…In addition to the antimicrobial, anti-inflammatory and inhibitory activities, pharmacological studies have shown that nsLTPs have antinociceptive activity, making them a promising alternative to current drugs available on the market that demonstrates high toxicity and low efficacy. McLTP1 obtained from M. citrifolia seeds demonstrated pain-related modulatory activity in vivo and did not show neurological disorders such as locomotor activity, suggesting that the nsLTP antinociceptive response was highly selective [ 87 ].…”

Section: Pltps As Antimicrobial Agentsmentioning

confidence: 99%

Lipid Transfer Proteins (LTPs)—Structure, Diversity and Roles beyond Antimicrobial Activity

et al. 2021

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Lipid transfer proteins (LTPs) are among the most promising plant-exclusive antimicrobial peptides (AMPs). They figure among the most challenging AMPs from the point of view of their structural diversity, functions and biotechnological applications. This review presents a current picture of the LTP research, addressing not only their structural, evolutionary and further predicted functional aspects. Traditionally, LTPs have been identified by their direct isolation by biochemical techniques, whereas omics data and bioinformatics deserve special attention for their potential to bring new insights. In this context, new possible functions have been identified revealing that LTPs are actually multipurpose, with many additional predicted roles. Despite some challenges due to the toxicity and allergenicity of LTPs, a systematic review and search in patent databases, indicate promising perspectives for the biotechnological use of LTPs in human health and also plant defense.

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First isolation and antinociceptive activity of a lipid transfer protein from noni ( Morinda citrifolia ) seeds

Cited by 22 publications

References 55 publications

Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats

Anti-nociceptive effect of total alkaloids isolated from the seeds of Areca catechu L (Arecaceae) in mice

Lipid Transfer Proteins (LTPs)—Structure, Diversity and Roles beyond Antimicrobial Activity

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