First Investigation of the Optimal Timing of Vaccination of Nile Tilapia (Oreochromis niloticus) Larvae against Streptococcus agalactiae
Benchawan Kumwan,
Anurak Bunnoy,
Satid Chatchaiphan
et al.
Abstract:To investigate early immune responses and explore the optimal vaccination periods, Nile tilapia at 1, 7, 14, 21, 28, 35, and 42 days after yolk sac collapse (DAYC) were immersed in formalin-killed Streptococcus agalactiae vaccine (FKV-SA). A specific IgM was first detected via ELISA in the 21 DAYC larvae (0.108 g) at 336 h after vaccination (hav), whereas in the 28–42 DAYC larvae (0.330–0.580 g), the specific IgM could be initially detected at 24 hav. qRT–PCR analysis of the TCRβ, CD4, MHCIIα, IgHM, IgHT, and … Show more
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