First insights into a type II toxin-antitoxin system from the clinical isolate Mycobacterium sp. MHSD3, similar to epsilon/zeta systems

Jaén‐Luchoro, Daniel; Aliaga-Lozano, Francisco; Gomila, Rosa M.; Gomila, Margarita; Salvà‐Serra, Francisco; Lalucat, Jorge; Bennasar, Antonio

doi:10.1371/journal.pone.0189459

Cited by 7 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the antagonistic pattern of toxins and antitoxins, the TAS can be divided into six types, and the above-mentioned TASs in this study are all type II systems, where the antitoxin post-translationally inhibits the toxin protein (55). Among these toxins, CcdB is responsible for the inhibition of DNA replication (38), and the others, PezT, SezT, and zeta toxin, are responsible for the repression of cell membrane formation (39)(40)(41). After characterization, PezT/PezA exhibited the desired inhibition and anti-inhibition of cell growth, indicating its adaptability for TAS construction.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Activation-induced cytidine deaminase-basedin vivocontinuous evolution system enables rapid protein engineering

Zhang

Liu

et al. 2023

Preprint

View full text Add to dashboard Cite

Directed evolution is a powerful tool to modify the properties of proteins. However, due to multi-round and stage combinations, directed evolution usually requires time- and labor-intensive manual intervention, which limits the efficiency of protein modification to some extent. Therefore,in vivocontinuous evolution system is highly preferred because it can couple the multiple rounds and steps of direction evolution with the host growth cycle, leading to the advantages of effort-saving and accuracy. However, the existing types of this kind of systems can not meet the booming demand. Herein, this paper describes promotedEscherichia coli-assisted continuous evolution (PEACE) that allows forin vivocontinuous evolution of target genes. This system polymorphisms the target gene by activation-induced cytidine deaminase-T7 RNA polymerase (AID-T7 PNAP) fusion protein, then it couples the enzymatic properties of desired variants with the expression of antitoxins to achieve efficient growth-coupled screen using the toxin-antitoxin system (TAS). In this study, T7 RNAP was finally employed for validation of PEACE system, and its specificity to the promoter was successfully altered. These results demonstrated the feasibility and further application potential of PEACE.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The genes encoding following toxin-antitoxins, CcdB/CcdA (38), PezT/PezA (39), SezT/SezA (40), and zeta toxin/epsilon (41), were synthesized, respectively. The vector pRSFDuet was employed as the expression vector backbone for the above proteins, respectively.…”

Section: Methodsmentioning

confidence: 99%

Activation-induced cytidine deaminase-basedin vivocontinuous evolution system enables rapid protein engineering

Zhang

Liu

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Each gel slice was cut into small pieces and then transferred to a clean and sterile Eppendorf tube. Protein identification was performed following Jaén-Luchoro et al ( 2017 ). The samples were then analyzed with an Autoflex III MALDI-TOF-TOF (BrukerDaltonics) spectrometer using the software Compassflex series v1.4 (flexControl v3.4, flexAnalysis v3.4 and BioTools 3.2).…”

Section: Methodsmentioning

confidence: 99%

“The Good, the Bad and the Double-Sword” Effects of Microplastics and Their Organic Additives in Marine Bacteria

et al. 2021

Self Cite

View full text Add to dashboard Cite

Little is known about the direct effects of microplastics (MPs) and their organic additives on marine bacteria, considering their role in the nutrient cycles, e.g., N-cycles through the N2-fixation, or in the microbial food web. To fill this gap of knowledge, we exposed marine bacteria, specifically diazotrophs, to pure MPs which differ in physical properties (e.g., density, hydrophobicity, and/or size), namely, polyethylene, polypropylene, polyvinyl chloride and polystyrene, and to their most abundant associated organic additives (e.g., fluoranthene, 1,2,5,6,9,10-hexabromocyclododecane and dioctyl-phthalate). Growth, protein overproduction, direct physical interactions between MPs and bacteria, phosphorus acquisition mechanisms and/or N2-fixation rates were evaluated. Cyanobacteria were positively affected by environmental and high concentrations of MPs, as opposed to heterotrophic strains, that were only positively affected with high concentrations of ~120 μm-size MPs (detecting the overproduction of proteins related to plastic degradation and C-transport), and negatively affected by 1 μm-size PS beads. Generally, the organic additives had a deleterious effect in both autotrophic and heterotrophic bacteria and the magnitude of the effect is suggested to be dependent on bacterial size. Our results show species-specific responses of the autotrophic and heterotrophic bacteria tested and the responses (beneficial: the “good,” deleterious: the “bad” and/or both: the “double-sword”) were dependent on the type and concentration of MPs and additives. This suggests the need to determine the threshold levels of MPs and additives concentrations starting from which significant effects can be observed for key microbial populations in marine systems, and these data are necessary for effective environmental quality control management.

show abstract

“…Antitoxins are the critical regulatory component for Type II systems, wherein they (typically) contain an N-terminal DNA binding motif used for autoregulation of the TA operon, and a less structured C-terminal domain to neutralize the toxin's activity. Selected systems that have the antitoxin domains reversed, such that the N-terminal region mediates toxin neutralization [89,[155][156][157][158][159], while some antitoxins are limited to only the toxin-binding domain [160]. Antitoxins neutralize toxins by either by blocking the active site or by causing conformation changes that prevent toxin interaction with the cellular target [51,154,161].…”

Section: Conservation Of Type II Ta System Folds and Cognate Antitoximentioning

confidence: 99%

Evaluating the Potential for Cross-Interactions of Antitoxins in Type II TA Systems

Holt

Ruan

2020

Toxins

View full text Add to dashboard Cite

The diversity of Type-II toxin–antitoxin (TA) systems in bacterial genomes requires tightly controlled interaction specificity to ensure protection of the cell, and potentially to limit cross-talk between toxin–antitoxin pairs of the same family of TA systems. Further, there is a redundant use of toxin folds for different cellular targets and complexation with different classes of antitoxins, increasing the apparent requirement for the insulation of interactions. The presence of Type II TA systems has remained enigmatic with respect to potential benefits imparted to the host cells. In some cases, they play clear roles in survival associated with unfavorable growth conditions. More generally, they can also serve as a “cure” against acquisition of highly similar TA systems such as those found on plasmids or invading genetic elements that frequently carry virulence and resistance genes. The latter model is predicated on the ability of these highly specific cognate antitoxin–toxin interactions to form cross-reactions between chromosomal antitoxins and invading toxins. This review summarizes advances in the Type II TA system models with an emphasis on antitoxin cross-reactivity, including with invading genetic elements and cases where toxin proteins share a common fold yet interact with different families of antitoxins.

show abstract

First insights into a type II toxin-antitoxin system from the clinical isolate Mycobacterium sp. MHSD3, similar to epsilon/zeta systems

Cited by 7 publications

References 54 publications

Activation-induced cytidine deaminase-basedin vivocontinuous evolution system enables rapid protein engineering

Activation-induced cytidine deaminase-basedin vivocontinuous evolution system enables rapid protein engineering

“The Good, the Bad and the Double-Sword” Effects of Microplastics and Their Organic Additives in Marine Bacteria

Evaluating the Potential for Cross-Interactions of Antitoxins in Type II TA Systems

Contact Info

Product

Resources

About