First identification and characterization of Tenacibaculum dicentrarchi isolated from Chilean red conger eel (Genypterus chilensis, Guichenot 1848)

A high‐throughput analysis of biofilm formation by the fish pathogen Tenacibaculum dicentrarchi

“…Piñeiro-Vidal, Gijón, Zarza, & Santos, 2012), but has also been identified in Atlantic salmon (Salmo salar) in Chile and Norway (Avendaño-Herrera et al, 2016;Olsen et al, 2017), as well as in red conger eel (Genypterus chilensis) in Chile (Irgang et al, 2017).…”

“…Tenacibaculum dicentrarchi-associated tenacibaculosis in fish is characterized by gross lesions on body surfaces, severe tail rots, frayed fins and other injuries (Avendaño-Herrera et al, 2016;Irgang et al, 2017;Piñeiro-Vidal et al, 2012). When subjected to challenge assays with T. dicentrarchi TdChD05, rainbow trout (Oncorhynchus mykiss) and Atlantic salmon present severe clinical signs and high mortality rates (Avendaño-Herrera et al, 2016).…”

“…Tenacibaculum dicentrarchi TdChD05 and Q CR29 were isolated from tail lesions in Atlantic salmon (Avendaño-Herrera et al, 2016) and from skin lesions in red conger eel (Irgang et al, 2017), respectively. The CECT 7612 T type strain, isolated from diseased European sea bass in Spain (Piñeiro-Vidal et al, 2012), was obtained from the Spanish Type Culture Collection (CECT) and included for comparative purposes.…”

“…The CECT 7612 T type strain, isolated from diseased European sea bass in Spain (Piñeiro-Vidal et al, 2012), was obtained from the Spanish Type Culture Collection (CECT) and included for comparative purposes. All strains were confirmed as T. dicentrarchi through standard phenotyping and 16S rDNA-based PCR (Avendaño-Herrera, Irgang, & Tapia-Cammas, 2018;Irgang et al, 2017). Stock cultures of T. dicentrarchi were prepared and stored at −80ºC in cryotubes (AES Laboratoire, Marcy-L'Étoile, France) using the Flexibacter maritimus medium (FMM) (Pazos, Santos, Macias, Nuñez, & Toranzo, 1996) amended with 15% (v/v) glycerol.…”

“…Tenacibaculum dicentrarchi ‐associated tenacibaculosis in fish is characterized by gross lesions on body surfaces, severe tail rots, frayed fins and other injuries (Avendaño‐Herrera et al, ; Irgang et al, ; Piñeiro‐Vidal et al, ). When subjected to challenge assays with T .…”