First Neisseria gonorrhoeae Genotyping Analysis in France: Identification of a Strain Cluster with Reduced Susceptibility to Ceftriaxone

Abstract

isolates. The major STs identified in France were previously described elsewhere. However, two newly described STs, ST1479 and ST1987, had only been found in France until now. ST1479 was characterized by a multipleresistance phenotype, whereas ST1987 presented a susceptibility phenotype. Moreover, among the predominant French STs, ST225, which had already been described in many countries, comprised isolates (14/16) resistant to ciprofloxacin and with reduced susceptibility to ceftriaxone. Thus, the surveillance of resistance to antibiotics is a priority in order to adapt treatment and decrease the transmission of resistant strains. Of note, no predominant ST was identified among rectal isolates from men who have sex with men.

Phenotypic and genotypic properties of Neisseria gonorrhoeae isolates in Norway in 2009: antimicrobial resistance warrants an immediate change in national management guidelines

“…For example, ST1407, which contains a penA mosaic allele, has been found to cause decreased susceptibility to ESCs in many countries worldwide [22]. Moreover, ST292 has been previously found in other European countries such as Sweden [22], France [23], and Scotland [24]. In general, the NG-MAST results also showed that the strains circulating in Norway were heterogeneous; 72 NG-MAST STs, 37 not previously described, were found among 114 isolates.…”

Analysis of Emergence of Quinolone-Resistant Gonococci in Greece by Combined Use of Neisseria gonorrhoeae Multiantigen Sequence Typing and Multilocus Sequence Typing

“…(2,9,10). However, the method has also been used, as it is here, to compare gonococcal isolates recovered from different years across whole countries (7,13,14,16,26). The method has some drawbacks for this purpose, as variant STs that differ at either porB or tbpB and then at both loci will rapidly be generated.…”

Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region

“…Based on this two-locus-combined profile, the isolates were equally assigned as ST225, which indicates a shared ancestral origin for these isolates. The ST225 seems to be an emerging multi-resistant clone that has been recently identified in many countries such as Australia (Todd et al 2007), Scotland (Palmer et al 2008), France (Monfort et al 2009), Portugal (Florindo et al 2010) and Greece (Mavroidi et al 2011). In fact, ST225 was the most prevalent ST in Scotland and France and has been reported as the second major genotype among QRNG isolates that were collected between 1997-2007 in Greece.…”

“…In addition, ST225 was recently associated with reduced susceptibility to ceftriaxone (Monfort et al 2009, Whiley et al 2010. Until now, the CLSI (2010b) did not stipuUntil now, the CLSI (2010b) did not stipuUntil now, the CLSI (2010b) did not stipudid not stipulate MIC values to reduced susceptibility or resistance to ceftriaxone.…”