First
            <i>De Novo</i>
            Draft Genome Sequence of the Pathogenic Fungus Fusarium udum F02845, Associated with Pigeonpea (Cajanus cajan L. Millspaugh) Wilt

This genome announcement includes draft genomes from Claviceps purpurea s.lat., including C. arundinis , C. humidiphila and C. cf. spartinae . The draft genomes of Davidsoniella eucalypti, Quambalaria eucalypti and Teratosphaeria destructans, all three important eucalyptus pathogens, are presented. The insect associate Grosmannia galeiformis is also described. The pine pathogen genome of Fusarium circinatum has been assembled into pseudomolecules, based on additional sequence data and by harnessing the known synteny within the Fusarium fujikuroi species complex. This new assembly of the F. circinatum genome provides 12 pseudomolecules that correspond to the haploid chromosome number of F. circinatum . These are comparable to other chromosomal assemblies within the FFSC and will enable more robust genomic comparisons within this species complex.

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“…2017a, b, Wingfield et al . 2017, Gardiner 2018, Srivastava et al 2018, Van Wyk et al . 2018, Wingfield et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Nine draft genome sequences of Claviceps purpurea s.lat., including C. arundinis, C. humidiphila, and C. cf. spartinae, pseudomolecules for the pitch canker pathogen Fusarium circinatum, draft genome of Davidsoniella eucalypti, Grosmannia galeiformis, Quambalaria eucalypti, and Teratosphaeria destructans

et al. 2018

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“…Whole Genome Sequencing of Host and FW Causing Pathogen: New Insights Into the Plant Defense System Against FW Availability of whole genome sequence information in chickpea (Varshney et al, 2013), common bean (Schmutz et al, 2014), cowpea (Lonardi et al, 2019), pea (Kreplak et al, 2019), and pigeonpea (Varshney et al, 2012) could allow identification of the candidate gene(s)/the genomic regions controlling disease resistance. Concurrently, draft genome assemblies of Fusarium udum F02845 (Srivastava et al, 2018) and Foc (Foc-38-1) and Fop (Fop-37622) (Williams et al, 2016) have shed new light onto virulence-related genes that enhance our current understanding…”

Section: Sequencing Based Approaches For Understanding the Plant-wiltmentioning

confidence: 99%

“…Subsequent advances in genomics accelerated molecular mapping of FW resistance gene(s)/QTLs. To this end, availability of whole genome sequences of both plant and pathogen has shed deep insights into the host-pathogen relationship through leveraging comparative genomics approach (Varshney et al, 2012(Varshney et al, , 2013Schmutz et al, 2014;Williams et al, 2016;Srivastava et al, 2018;Kreplak et al, 2019;Lonardi et al, 2019). In this review, we discuss the targeted improvement of plant resistance against FW using genomics-assisted approaches.…”

Section: Introductionmentioning

confidence: 99%

Breeding, Genetics, and Genomics Approaches for Improving Fusarium Wilt Resistance in Major Grain Legumes

et al. 2020

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Fusarium wilt (FW) disease is the key constraint to grain legume production worldwide. The projected climate change is likely to exacerbate the current scenario. Of the various plant protection measures, genetic improvement of the disease resistance of crop cultivars remains the most economic, straightforward and environmental-friendly option to mitigate the risk. We begin with a brief recap of the classical genetic efforts that provided first insights into the genetic determinants controlling plant response to different races of FW pathogen in grain legumes. Subsequent technological breakthroughs like sequencing technologies have enhanced our understanding of the genetic basis of both plant resistance and pathogenicity. We present noteworthy examples of targeted improvement of plant resistance using genomics-assisted approaches. In parallel, modern functional genomic tools like RNA-seq are playing a greater role in illuminating the various aspects of plant-pathogen interaction. Further, proteomics and metabolomics have also been leveraged in recent years to reveal molecular players and various signaling pathways and complex networks participating in host-pathogen interaction. Finally, we present a perspective on the challenges and limitations of highthroughput phenotyping and emerging breeding approaches to expeditiously develop FW-resistant cultivars under the changing climate.

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“…First time observations on wilt resistance in pigeonpea was reported by Butler in 1908 [33]. Apart from this, 950 genotypes screened had shown zero resistance to FW with less than 10% wilt incidence in 19 genotypes [29], 16 out of 31 pigeonpea cultivars screened were resistant to wilt with highest degree of resistance (2.15 %) in BWR 369 cultivar [34]; six cultivars were reported to be resistant to FW among 216 late maturing pigeonpea germplasm evaluated [7]; resistance screening in 976 genotypes, germplasm and breeding lines FW using wilt sick plot confirmed 92 genotypes resistant after a rigorous two years testing at Patancheru, India [35]. Another study on evaluation of new elite pigeonpea germplasm using wilt sick plots demonstrated a consistent rate of high level of resistance (DI < 20.0 %) in ICEAP-00040 genotype to FW reported in Kenya, Malawi and Tanzania [31].…”

Section: Discussionmentioning

confidence: 97%

“…A number of resistance sources of germplasm against FW were identified by screening various pigeonpea genotypes over the last two decades at national and international level. Some of these promising genotypes were effectively used in crop improvement programs [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Molecular insights on differential responses of antioxidant and pathogenesis-related genes in pigeonpea [Cajanus cajan (L.) Millsp.] to Fusarium wilt (Fusarium udum (L.)

Biswas

Kumar

Tarafdar³

et al. 2019

Preprint

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Background: Pigeonpea [Cajanus cajan (L.) Millsp.] is a major dietary protein source to a large vegetarian population in the world. However, Fusarium wilt (FW) caused by Fusarium udum Butler (FU) is one of the challenging factors to pigeonpea production. FW resistance in pigeonpea is restricted to specific agro-climatic zones. Therefore, exploring a superior pigeonpea genotype from landraces or local cultivars cultivated in various rural regions and validating its resistance response at biological and molecular level could be an alternative to crop improvement program. The present study was carried out to identify superior genotypes of pigeonpea through selection of innate resistance to FW using different biological and molecular approaches.Result: Five distinct genotypes of pigeonpea along with well-known one resistant (ICP2894) and susceptible (ICP2376) control were selected on the basis of percentage of FW disease incidence from three different states of India after an extensive survey work. Among them, Richa had shown very less FW incidence (10.0%) under genotype evaluation study and was further selected for experimental validation at molecular level for its innate resistance to FW. Molecular characterization and expression profiling of biotic stress responsive genes representing antioxidant enzymes (APX and SOD) and Pathogenesis related proteins (CHS and β-1,3-glucanase)families were carried out. Under FU challenged conditions in Richa, expression patterns of both AO genes found to be similar, while expression of both PR genes was gradually increased with inoculation time. Putative 3D protein structure of CHS and SOD were generated. Molecular docking with fungal effect or protein cellobio-hydrolase-c (CBH-c) also revealed that their role in blocking the hydrolytic activity of CBH-c during pathogen invasion. Conclusion: The obtained results of expression study and in-silico analysis showed that the higher level of induction of PR and AO genes and strong interaction of their putative proteins with fungal CBH-cprotein established their antifungal activity conferring early plant defense responses to restrict the pathogen growth in resistant genotype Richa. Our study displaying a strong and combinatorial approach involving biological assay, molecular experiment and in-silico analysis to identify a superior genotype of pigeonpea resistant to FW across a major biogeographic region.

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First De Novo Draft Genome Sequence of the Pathogenic Fungus Fusarium udum F02845, Associated with Pigeonpea (Cajanus cajan L. Millspaugh) Wilt

Abstract: Fusarium udum F02845 is a destructive fungal pathogen which causes pigeonpea (Cajanus cajan L. Millspaugh) wilt.

Cited by 10 publications

References 14 publications

Breeding, Genetics, and Genomics Approaches for Improving Fusarium Wilt Resistance in Major Grain Legumes

Molecular insights on differential responses of antioxidant and pathogenesis-related genes in pigeonpea [Cajanus cajan (L.) Millsp.] to Fusarium wilt (Fusarium udum (L.)

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