First genome sequence of an isolate of hibiscus chlorotic ringspot virus from the Western hemisphere

Ramos-González, Pedro Luis; Costa-Rodrigues, Mariane da; Potsclam-Barro, M; Chabi-Jesus, Camila; Banguela-Castillo, Alexander; Harakava, Ricardo; Kitajima, Elliot Watanabe; Freitas-Astúa, Juliana

doi:10.1007/s40858-019-00331-x

Cited by 4 publications

(7 citation statements)

References 26 publications

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“…Computational analysis done on the HCRSV-UKM genome revealed that it had a nucleotide similarity ranging from approximately 87 to 93% with the isolates from Singapore (NC_003608.1), USA (MT512573.1), Brazil (MK279671.1), China (KY933060.1), Israel (KC876666.1), and Taiwan (DQ392986.1) [7,15,21,38]. The highest nucleotide sequence similarity of HCRSV-UKM was with the HCRSV from Singapore (93.37%), while the lowest sequence similarity was with the HCRSV from Taiwan (87.90%) (Table 3).…”

Section: The Nucleotide Sequence Of Hcrsv-malaysian Isolatementioning

confidence: 99%

“…The highest nucleotide sequence similarity of HCRSV-UKM was with the HCRSV from Singapore (93.37%), while the lowest sequence similarity was with the HCRSV from Taiwan (87.90%) (Table 3). Figure 2 outlines the identity plot of the HCRSV isolates showing the general overview of their nucleotide sequence identity [7]. In addition to the nucleotide sequence similarity, the amino acid sequence could also be deduced and compared using the ORF prediction.…”

Section: The Nucleotide Sequence Of Hcrsv-malaysian Isolatementioning

confidence: 99%

“…The putative p23 had the lowest percentage of amino acid sequence similarity. All the predicted amino acid sequences were put through the motif scan tools at ExPASy-MyHits (https://myhits.isb-sib.ch/cgi-bin/motif_scan, accessed on 1 March 2023) [7]. The results of the motif scan show that the RdRp and CP both have the potential for the catalytic domain and shell (S) domain, respectively.…”

Section: The Nucleotide Sequence Of Hcrsv-malaysian Isolatementioning

confidence: 99%

“…Hibiscus rosa-sinensis L. is a malvaceous perennial shrub that has been cultivated as ornamentals in different countries across multiple regions, such as Malaysia, Singapore, India, China, Hawaii, Brazil, Iran, and Nigeria [1][2][3][4][5][6][7]. H. rosa-sinensis is susceptible to plant viruses including Hibiscus chlorotic ringspot virus (HCRSV), Hibiscus latent Singapore virus (HLSV), Hibiscus latent ringspot virus (HLRSV), Hibiscus latent Fort Pierce virus (HLFPV), and Hibiscus green spot virus (HGSV) [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…The occurrence of the HCRSV in H. rosa-sinensis was first identified in the cultivars exported from El Salvador into the United States of America [12]. Since then, the virus has been identified in H. rosa-sinensis cultivated throughout the globe [6,7,15,16,[18][19][20][21][22][23]. A distinct characteristic of the HCRSV is that the virus can encode a total of seven open reading frames (ORFs): the typical five of a Carmovirus, in addition to two novel ORFs, namely, ORF (p23) and ORF (p25) [15,24].…”

Section: Introductionmentioning

confidence: 99%

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The Complete Genome Sequence, Molecular Detection, and Anatomical Analysis of Hibiscus Chlorotic Ringspot Virus Infecting Hibiscus rosa-sinensis in Peninsular Malaysia

Yusop,

Lanisa,

Talip

et al. 2023

Horticulturae

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Hibiscus rosa-sinensis is the national flower of Malaysia and is widely cultivated as landscape planting across the nation. In 1995, Hibiscus chlorotic ringspot virus (HCRSV) was reported for the first time in Malaysia. Until today, there have been no follow-up studies on the viral infection in the plant, yet the virus symptom of chlorotic spots has been observed throughout the Hibiscus population. Therefore, this study aimed to report the complete genome sequence of the HCRSV, validate a molecular detection tool for its diagnosis, and measure the impact of the virus symptom and infection in H. rosa-sinensis. This study reported the complete genome of the HCRSV through RNA sequencing. The phylogenetic analysis of the HCRSV isolated from different geographical sources and several other related viruses was performed to confirm its identity and classification. Additionally, primers based on the genome sequence were designed and used for RT-PCR to detect the presence of the virus in symptomatic leaves, further confirming the HCRSV identity and presence. Meanwhile, the impact of the virus was measured by examining the anatomical and morphological changes in the leaf structure of symptomatic samples. Light microscopy and scanning electron microscopy were used to examine potential adaptations and comparisons between the leaf structures of healthy and infected samples, especially in the leaf lamina, petiole, and midrib cells. The results confirmed the complete genome sequence of the HCRSV, its molecular detection strategy, and the impact of the viral infection on Hibiscus leaves in Peninsular Malaysia. This study is beneficial for the management strategy of the virus and for protecting an important plant in the nation.

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Section: The Nucleotide Sequence Of Hcrsv-malaysian Isolatementioning

confidence: 99%

Section: The Nucleotide Sequence Of Hcrsv-malaysian Isolatementioning

confidence: 99%

Section: The Nucleotide Sequence Of Hcrsv-malaysian Isolatementioning

confidence: 99%