First field evidence infection of Culex perexiguus by West Nile virus in Sahara Oasis of Algeria

Benbetka, S; Hachid, Aissam; Benallal, Kamal Eddine; Benbetka, Chahrazed; Khaldi, Abdelhamid; Bitam, Idir; Harrat, Zoubir

doi:10.4103/0972-9062.256566

Cited by 11 publications

(11 citation statements)

References 4 publications

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“…pipiens in Tunisia (Monastiri et al, 2018), 0.56% for Cx. perexiguus in Algeria (Benbetka et al, 2018), 0.24% for Cx. interrogator and 0.28% for Cx.…”

Section: Discussionmentioning

confidence: 98%

Evidence of circulation of West Nile virus in Culex pipiens mosquitoes and horses in Morocco

et al. 2020

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West Nile virus (WNV) is one of the most widely distributed mosquito-borne viruses in the world. In North Africa, it causes human cases of meningoencephalitis with fatalities in Algeria and in Tunisia, whereas only horses were affected in Morocco. The aims of this study were to detect WNV in mosquitoes and to determine seroprevalence of WNV in Moroccan horses by the detection of IgG antibodies. A total of 1,455 mosquitoes belonging to four different species were grouped by collection site, date, and sex with 10 specimens per pool and tested for 38 arboviruses using a high-throughput chip based on the BioMark Dynamic array system. Out of 146 mosquito pools tested, one pool was positive for WNV. This positive pool was confirmed by real time RT-PCR. The serosurvey showed that 33.7% (31/92) of horses were positive for competitive enzyme-linked immunosorbent assay (cELISA) test. The flavivirus-sphere microsphere immnoassay (MIA) test, targeting three flaviviruses (WNV, Usutu virus (USUV) and Tick borne encephalitic virus (TBEV)) showed that 23 sera out of 31 were positive for WNV, two for USUV, two for USUV or WNV, and four for an undetermined flavivirus. Virus neutralization tests with USUV and WNV showed that 28 of 31 sera were positive for WNV and all sera were negative for USUV. This study reports, for the first time, the detection of WNV from Culex pipiens mosquitoes in Morocco and its circulation among horses. This highlights that the detection of arboviruses in mosquitoes could serve as an early warning signal of a viral activity to prevent future outbreaks in animals and humans.

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“…pipiens in Tunisia (Monastiri et al, 2018), 0.56% for Cx. perexiguus in Algeria (Benbetka et al, 2018), 0.24% for Cx. interrogator and 0.28% for Cx.…”

Section: Discussionmentioning

confidence: 98%

Evidence of circulation of West Nile virus in Culex pipiens mosquitoes and horses in Morocco

et al. 2020

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“…Culex perexiguus a été collectée d'un seul gîte naturel: retenue collinaire, dans la commune de Makouda. En Algérie, elle a été trouvée également à Tébessa par Bouabida et al (2012), Souk-Ahras par Hamaidia & Berchi (2018), El Tarf dans le lac Tonga par Amara Korba et al (2015), Bousaada par Benhissen et al (2018), et dans les Oasis du Sahara, où elle a été signalée porteuse du virus West Nile par Benbetka et al (2018). Selon Brunhes et al (1999) (Brunhes et al, 1999).…”

Section: Resultats Et Discussionunclassified

“…La présence de cette espèce peut engendrer un risque réel, lié à l'apparition des épidémies de Chikungunia et de Dengue (Delaunay et al, 2009). Dans ce contexte, plusieurs travaux ont été réalisés sur les Culicidae en Algérie, notamment ceux de Rehimi & Soltani (1999), Messai et al (2010), Izri et al (2011), Berchi et al (2012), Bouabida et al (2012), Lafri et al (2014), Benallal et al (2015Benallal et al ( ,2016Benallal et al ( , 2019, Benbetka et al (2018), Benhissen et al (2018), Hamaidia & Berchi (2018), Nabti & Bounechada (2019), Kharoubi et al (2020). La présente étude a été consacrée à la détermination des espèces Culicidiennes inventoriées au niveau de la région de Tizi-Ouzou (Nord d'Algérie), et l'exploitation des résultats par les indices écologiques afin de préciser l'abondance relative et les fréquences d'occurrence.…”

Section: Introductionunclassified

Etude sur la faune Culicidienne (Diptera: Culicidae) de la région de Tizi-Ouzou (Nord d’Algérie) : Biodiversité, abondance et répartition

Chahed¹,

Brahmi²,

Djouaher³

2021

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Dans le but d’améliorer nos connaissances sur la diversité spécifique de la faune Culicidienne au niveau de la région de Tizi-Ouzou (Nord d’Algérie), nous avons mené un échantillonnage bimensuel dans 20 gîtes larvaires différents au cours d’une période allant du mois de février 2019 jusqu’au mois de juin 2020. Treize (13) espèces ont été recensées (Aedes (Ochlerotatus) caspius (Pallas, 1771), Aedes (Finlaya) geniculatus (Olivier, 1791), Anopheles maculipennis s.l. Meigen, 1818, Anopheles (Anopheles) claviger (Meigen, 1804), Culex (Culex) pipiens Linnaeus,1758, Culex (Maillotia) deserticola Kirkpatrick, 1925, Culex (Maillotia) hortensis Ficalbi, 1889, Culex (Neoculex) impudicus Ficalbi, 1890, Culex (Neoculex) territans Walker,1856, Culex (Culex) perexiguus (Theobald, 1903), Culiseta (Allotheobaldia) longiareolata (Macquart, 1838), Culiseta (Culiseta) subochrea (Edwards, 1921), Uranotaenia (Pseudoficalbia) unguiculata (Edwards,1913) appartenant à cinq genres (Aedes, Anopheles, Culex, Culiseta, Uranotaenia). Culex pipiens est l’espèce la plus abondante avec une fréquence relative de 44,53%, suivie par Culiseta longiareolata avec 41,96%.

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“…Previously in this region, the West Nile virus has been detected in Cx. perexiguus [ 23 ], and WNV human cases were reported [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Detection and Isolation of Sindbis Virus from Field Collected Mosquitoes in Timimoun, Algeria

Ayhan

Hachid

Thirion

et al. 2022

Viruses

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Sindbis virus (SINV) is a zoonotic alphavirus (family Togaviridae, genus Alphavirus) that causes human diseases in Africa, Europe, Asia, and Australia. Occasionally, SINV outbreaks were reported in South Africa and northern Europe. Birds are the main amplifying hosts of SINV, while mosquitoes play the role of the primary vector. Culex mosquitoes were collected in Algeria and subsequently tested for SINV. SINV RNA was detected in 10 pools out of 40, from a total of 922 mosquitoes tested. A strain of SINV was isolated from a pool displaying high viral load. Whole-genome sequencing and phylogenetic analysis showed that the SINV Algeria isolate was most closely related to a Kenyan strain. This was the first record of SINV in Algeria and more broadly in northwestern Africa, which can be a potential risk for human health in the circulating area. Further studies are needed to measure the impact on public health through seroprevalence studies in Algeria.

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First field evidence infection of Culex perexiguus by West Nile virus in Sahara Oasis of Algeria

Cited by 11 publications

References 4 publications

Evidence of circulation of West Nile virus in Culex pipiens mosquitoes and horses in Morocco

Evidence of circulation of West Nile virus in Culex pipiens mosquitoes and horses in Morocco

Etude sur la faune Culicidienne (Diptera: Culicidae) de la région de Tizi-Ouzou (Nord d’Algérie) : Biodiversité, abondance et répartition

Detection and Isolation of Sindbis Virus from Field Collected Mosquitoes in Timimoun, Algeria

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