First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis

Carabarín-Lima, Alejandro; León-Izurieta, Libia; Rocha-Gracia, Rosa del Carmen; Castañeda‐Lucio, Miguel; Torres, Carmén; Gutiérrez-Cazarez, Zita; González-Posos, Sirenia; Peña, Claudia F. Martínez de la; Martínez‐Laguna, Ygnacio; Lozano-Zaraín, Patricia

doi:10.1099/jmm.0.000291

Cited by 12 publications

(4 citation statements)

References 47 publications

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“… 36 Transmission electron microscopy with Ryu staining also showed that KLP JBUAP021 expresses polar flagella. 41 However, we did not find the involvement of NLRC4 in KLPJ- mediated colitis. Other studies have shown that NLRP3 inflammasomes were involved in regulating colonic immune homeostasis; Huber et al 51 showed that both NLRP3 and NLRP6 inflammasomes mediated production of IL18.…”

Section: Discussioncontrasting

confidence: 73%

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Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11–Mediated IL18 in the Gut Epithelial Cells

Zhang¹,

Su²,

Zhang³

et al. 2023

Cellular and Molecular Gastroenterology and Hepatology

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Section: Discussioncontrasting

confidence: 73%

“…NLRC4 is essential for the KLP -induced production of IL1β, IL17A, and neutrophil chemoattractants in the lungs. 36 Certain strains of KLP have been shown to possess flagella, 41 and flagella can activate NLRC4. 42 However, the data did not show the involvement of NLRC4 in KLPJ- mediated colitis ( Figure 8 B ).…”

Section: Resultsmentioning

confidence: 99%

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11–Mediated IL18 in the Gut Epithelial Cells

Zhang¹,

Su²,

Zhang³

et al. 2023

Cellular and Molecular Gastroenterology and Hepatology

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“…coli , floating biofilm formation has been associated with the presence of curli, flagella, Type 1 pili, siderophores, exopolysaccharide and the sensor kinase QseC [33], and recently, the presence of polar flagella has also been reported in one K . pneumoniae isolate [34]. Despite that, to our knowledge, this is the first time hypervirulent-hypermucoviscous K .…”

Section: Discussionmentioning

confidence: 99%

Hypervirulent Klebsiella pneumoniae serotype K1 clinical isolates form robust biofilms at the air-liquid interface

et al. 2019

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The prevalence of a new hypervirulent and hypermucoviscous K. pneumoniae phenotype (Hmv) is increasing worldwide, mainly linked to serotypes K1 and K2. Since capsular thickness can directly affect the capability to form biofilms, we aimed to evaluate the association between the Hmv phenotype with adhesion and biofilm formation in a collection of clinical K. pneumoniae isolates.We selected 38 Hmv clinical isolates [15 serotype K1; 9 serotype K2; 3 non-K1/K2 (rmpA+); 11 non-K1/K2 (rmpA-)] and 7 non-Hmv clinical isolates. The Hmv phenotype was assessed through the mucoviscosity test. Serum resistance was determined by bacterial viability tests in pooled human serum. Adhesion was evaluated with the Biofilm Ring Test®, and biofilm formation was identified by crystal violet staining (Solid-Liquid, SLI-biofilm) or visual examination (Air-Liquid, ALI-biofilm).This study linked for the first time the formation of robust ALI-biofilm plugs by K. pneumoniae to the capsular serotype K1, a group of hypervirulent strains which are generally highly susceptible to the antimicrobial agents. Among all the studied isolates, the capsular serotype K1 presented lower initial adhesion despite having the adhesins mrkD and fimH but higher ALI-biofilm formation than isolates with other capsular serotypes (K2 or non-K1/K2). This structure might confer increased resistance to a group of hypervirulent K. pneumoniae serotype K1.

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“…pneumoniae is usually a non-flagellated bacterium, although a flagella expressing K . pneumoniae isolate was identified in 2016 [86]. A .…”

Section: Resultsmentioning

confidence: 99%

Sugar and iron: Toward understanding the antibacterial effect of ciclopirox in Escherichia coli

Conley¹,

Carlson-Banning²,

Carter³

et al. 2019

PLoS ONE

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New antibiotics are needed against antibiotic-resistant gram-negative bacteria. The repurposed antifungal drug, ciclopirox, equally blocks antibiotic-susceptible or multidrug-resistant Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae clinical isolates, indicating that it is not affected by existing resistance mechanisms. Toward understanding how ciclopirox blocks growth, we screened E. coli mutant strains and found that disruption of genes encoding products involved in galactose salvage, enterobacterial common antigen synthesis, and transport of the iron binding siderophore, enterobactin, lowered the minimum inhibitory concentration of ciclopirox needed to block growth of the mutant compared to the isogenic parent strain. We found that ciclopirox induced enterobactin production and that this effect is strongly affected by the deletion of the galactose salvage genes encoding UDP-galactose 4-epimerase, galE, or galactose-1-phosphate uridylyltransferase, galT. As disruption of ECA synthesis activates the regulation of capsular synthesis (Rcs) phosphorelay, which inhibits bacterial swarming and promotes biofilm development, we test whether ciclopirox prevents activation of the Rcs pathway. Sub-inhibitory concentrations of ciclopirox increased swarming of the E. coli laboratory K12 strain BW25113 but had widely varying effects on swarming or surface motility of clinical isolate E. coli, A. baumannii, and K. pneumoniae. There was no effect of ciclopirox on biofilm production, suggesting it does not target Rcs. Altogether, our data suggest ciclopirox-mediated alteration of lipopolysaccharides stimulates enterobactin production and affects bacterial swarming.

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First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis

Cited by 12 publications

References 47 publications

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11–Mediated IL18 in the Gut Epithelial Cells

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11–Mediated IL18 in the Gut Epithelial Cells

Hypervirulent Klebsiella pneumoniae serotype K1 clinical isolates form robust biofilms at the air-liquid interface

Sugar and iron: Toward understanding the antibacterial effect of ciclopirox in Escherichia coli

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