First evidence for the production of cylindrospermopsin and deoxy-cylindrospermopsin by the freshwater benthic cyanobacterium, Lyngbya wollei (Farlow ex Gomont) Speziale and Dyck

Seifert, Mark; McGregor, Glenn B.; Eaglesham, Geoff; Wickramasinghe, W. A.; Shaw, Glendon Reginald

doi:10.1016/j.hal.2006.07.001

Cited by 186 publications

(124 citation statements)

References 57 publications

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“…Cylindrospermopsin (CYN) is one of the common members of cyanotoxins which always cause human injury. CYN originates from several cyanobacteria, such as Cylindrospermopsis raciborskii [3], Umezakia natans [4], Aphanizomenon ovalisporum [5], Anabaena bergii [6], Raphidiopsis curvata [7], Aphanizomenon flos-aquae [8], Anabaena lapponica [9], Lyngbya wollei [10] and Aphanizomenon gracile [11]. CYN has neurotoxic effects (inhibition of protein synthesis by binding to liver DNA and forms single DNA adducts which results in liver damage), hepatotoxic effects (inhibition of glutathione synthesis in hepatocytes), and cytotoxic effects (inhibitor of cytochrome P450) [12].…”

Section: Introductionmentioning

confidence: 99%

Bismuth oxybromide promoted detoxification of cylindrospermopsin under UV and visible light illumination

Wang

Fang

et al. 2014

Applied Catalysis B: Environmental

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Section: Introductionmentioning

confidence: 99%

Bismuth oxybromide promoted detoxification of cylindrospermopsin under UV and visible light illumination

Wang

Fang

et al. 2014

Applied Catalysis B: Environmental

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“…It should be noted that-at least in Spain-CYN has been observed only in basic bodies of water, namely Arcos reservoir and the pond in Parque de Juan Carlos I (Quesada et al 2006;Wormer et al 2008). Also the appearance of CYN-producing organisms in Shaw et al (1999) or Seifert et al (2007) is related to sites presenting pH values above 7. Sample preparation thus seems to be most needed in the types of bodies of water that have been described to house CYN-producing organisms.…”

Section: Discussionmentioning

confidence: 99%

“…Among these species, the most widely distributed is Cylindrospermopsis raciborskii, which is now found in many countries (Briand et al 2004). Other species producing this cyanotoxin are Umezakia natans (Harada et al 1994), Anabaena bergii (Schembri et al 2001), Raphidiopsis curvata (Li et al 2001), Aphanizomenon ovalisporum (Banker et al 1997), Aphanizomenon flos-aquae (Preussel et al 2006), Anabaena lapponica (Spoof et al 2006), or Lyngbya wollei (Seifert et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Advances in solid phase extraction of the cyanobacterial toxin cylindrospermopsin

Wörmer

Carrasco

Cirés

et al. 2009

Limnology & Ocean Methods

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Unlike other cyanotoxins, cylindrospermopsin (CYN) is often found in high concentrations in the extracellular fraction, possibly due to a higher release from the producing cells and a limited degradation once liberated. This fact demands effective recovery methods from environmental samples to guarantee a proper quantification. A reliable and simple solid phase extraction (SPE) method was obtained by the sole use of graphitized carbon cartridges and by paying special attention to both the selection of the most suitable solvent and the need of sample preparation prior to SPE. An acidified combination of dichloromethane and methanol not only showed best recoveries, but also allowed drastic reduction to the elution volumes needed with complete recovery being achieved in only 8 mL. Acidification of the sample and addition of sodium chloride are suggested as valuable improvements to the SPE method and turn out to be essential for correct and robust recovery of CYN in environmental samples of great diversity in terms of pH, DOC, and conductivity. Excellent behavior of the proposed method over a wide range of CYN loadings, applied sample volumes, and DOC loadings was also confirmed.

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“…The freshwater cyanobacteria, L. wollei is a source of biologically active substances including toxic analogues of the saxitoxins such as dcSTX, dcGTX2, dcGTX3 and LWTXs, as well as of the hepatotoxin deoxy-CYN [17][18][19][20][21]. L. aerugineo-coerulea has been also defined as a potential producer of extracellular and intracellular toxic substances [22].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii

et al. 2013

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Cyanobacteria are prokaryotic organisms characterized by their ability to produce secondary metabolites with different biological activities. The aim of this work was to evaluate the in vitro and in vivo toxicity of the cosmopolitan freshwater cyanobacterium H. kuetzingii. An extract from H. kuetzingii and cyanobacterial growth media were assessed for presence of intracellular and extracellular toxins by in vitro tests using primary cell cultures from mouse kidney and fibroblasts, cell lines A549 and 3T3, a fish cell line RTgill-W1 as well as by a traditional in vivo mouse bioassay. The presence of toxicity was compared with the ELISA and HPLC data for corresponding cyanotoxins. In vitro tests showed pronounced cytotoxicity of the cyanobacterium extract and growth medium in which H. kuetzingii released potential extracellular toxic compounds as the mammalian cells were significantly more sensitive to exposure compared to the fish cells. Histopathological analyses of the liver and kidneys of treated mice showed pathological changes such as leukocyte infiltration and necrosis, changes in the proximal and distal convoluted tubules, lack of differentiation of Bowman's space, enlarged Bowman's capsules and massive hemorrhages. ELISA and HPLC analyses confirmed the presence of saxitoxins and microcystins at low concentrations. In addition, the histological analyses suggest that H. kuetzingii produces other, yet unknown toxic metabolites. Monitoring efforts are therefore required to evaluate the potential hazard for the freshwater aquatic systems and possible public health implications associated with this cyanobacterium.

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First evidence for the production of cylindrospermopsin and deoxy-cylindrospermopsin by the freshwater benthic cyanobacterium, Lyngbya wollei (Farlow ex Gomont) Speziale and Dyck

Cited by 186 publications

References 57 publications

Bismuth oxybromide promoted detoxification of cylindrospermopsin under UV and visible light illumination

Bismuth oxybromide promoted detoxification of cylindrospermopsin under UV and visible light illumination

Advances in solid phase extraction of the cyanobacterial toxin cylindrospermopsin

In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii

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