First electroanalytical studies of methoxyfenozide and its interactions with dsDNA

Morawska, Kamila; Ciesielski, Witold; Smarzewska, Sylwia

doi:10.1016/j.jelechem.2021.115030

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…As a result, it could be assumed that part of the binding sites were buried within the interface of the produced complex. The quenching constants could be analyzed by the application of the Stern-Volmer equation as follows [30] :…”

Section: Fluorescence Quenching Studiesmentioning

confidence: 99%

Evaluation of the binding effect and cytotoxicity assay of 2‐Ethyl‐5‐(4‐methylphenyl) pyramido pyrazole ophthalazine trione on calf thymus DNA: spectroscopic, calorimetric, and molecular dynamics approaches

et al. 2021

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With advances in new drug therapies, it is essential to understand the interactions between drugs and target molecules. In this study, we applied multiple spectroscopic techniques including absorbance, fluorescence, circular dichroism spectroscopy, viscosity, thermal melting, calorimetric, and molecular dynamics (MD) simulation to study the interaction between 2-Ethyl-5-(4-methylphenyl) pyramido pyrazole ophthalazine trione (PPF) and calf thymus DNA (ct DNA) in the absence or presence of histone H1. PPF exhibits a high binding affinity towards ct DNA in binary and ternary systems. In addition, the result for the binding constant was observed within the range 10 4 M À1 achieved through fluorescence quenching data, while the values for enthalpy and entropy changes for ct DNA-PPF and (ct DNA-H1) PPF complexes were measured to be À72.54 kJ.mol À1 , À161.14 J.mol À1 K À1 , À85.34 kJ.mol À1 , and À19.023 J.mol À1 K À1 , respectively. Furthermore, in accordance with circular dichroism spectra, the inducement of ct DNA structural changes was observed during binding of PPF and H1 in binary and ternary system forms. The essential roles of hydrogen bonding and van der Waals forces throughout the interaction were suggested using thermodynamic parameters. According to the obtained data, the interaction mode of ct DNA-PPF and (ct DNA-H1) PPF complexes was intercalation binding. Suggested by the MD simulation study, the ct DNA-H1 complex caused a reduction in the stability of the DNA structure in the presence or absence of ligand, which demonstrated that PPF as an intercalating agent can further distort the structure. The information achieved from this study will be very helpful in understanding the effects of PPF on the conformational state of ct DNA in the absence or presence of the H1 molecule, which seems to be quite significant for clarifying the mechanisms of action and its pharmacokinetics.

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Section: Fluorescence Quenching Studiesmentioning

confidence: 99%

Evaluation of the binding effect and cytotoxicity assay of 2‐Ethyl‐5‐(4‐methylphenyl) pyramido pyrazole ophthalazine trione on calf thymus DNA: spectroscopic, calorimetric, and molecular dynamics approaches

et al. 2021

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“…Salmon sperm DNA has a double-stranded B-DNA structure composed of four types of nucleotides (A, T, G, and C), and it is widely used as the representative DNA in the environment for various studies, including its binding interaction with organic pollutants [ 27 , 28 ], adsorption on clay minerals [ 29 , 30 ], and aggregation in water environment [ 31 , 32 ]. This kind of DNA is, therefore, selected in this study.…”

Section: Methodsmentioning

confidence: 99%

Insights into the enzymatic degradation of DNA expedited by typical perfluoroalkyl acids

Qin,

Zhang,

et al. 2023

Eco-Environment & Health

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“…The recorded response results from direct transformation of the desired chemical information (concentration, activity) into a current signal (potential, current, resistance, or capacity), according to the selected method. Voltammetry is considered as one of the most sensitive electroanalytical methods, suitable for the determination of trace amounts of many metals and compounds in clinical, industrial, and environmental samples [ 1 , 2 , 3 , 4 ]. Various voltammetric techniques provide a wealth of chemical, electrochemical, and physical information, such as quantitative analysis, diffusion and reaction rate constants, and number of electrons involved in redox reactions [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Graphene Oxide Composition on Properties of Surface-Modified Metal Electrodes

et al. 2022

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The present paper describes the effect of the concentration of two graphene oxides (with different oxygen content) in the modifier layer on the electrochemical and structural properties of noble metal disk electrodes used as working electrodes in voltammetry. The chemistry of graphene oxides was tested using EDS, FTIR, UV–Vis spectroscopy, and combustion analysis. The structural properties of the obtained modifier layers were examined by means of scanning electron and atomic force microscopy. Cyclic voltammetry was employed for comparative electrochemical studies.

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First electroanalytical studies of methoxyfenozide and its interactions with dsDNA

Cited by 7 publications

References 43 publications

Evaluation of the binding effect and cytotoxicity assay of 2‐Ethyl‐5‐(4‐methylphenyl) pyramido pyrazole ophthalazine trione on calf thymus DNA: spectroscopic, calorimetric, and molecular dynamics approaches

Evaluation of the binding effect and cytotoxicity assay of 2‐Ethyl‐5‐(4‐methylphenyl) pyramido pyrazole ophthalazine trione on calf thymus DNA: spectroscopic, calorimetric, and molecular dynamics approaches

Insights into the enzymatic degradation of DNA expedited by typical perfluoroalkyl acids

The Influence of Graphene Oxide Composition on Properties of Surface-Modified Metal Electrodes

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