First Draft Genome Sequence of a Mycobacterium gordonae Clinical Isolate

Ustinova, Vera V.; Smirnova, T. G.; Blagodatskikh, Konstantin A.; Varlamov, D. A.; Сочивко, Д. Г.; Ларионова, Е. Е.; Andreevskaya, Sofya; Andrievskaya, I.; Chernousova, L. N.

doi:10.1128/genomea.00638-16

Cited by 5 publications

(2 citation statements)

References 10 publications

(9 reference statements)

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“…A number of Actinobacteriota that encoded RuBisCO form IE and trace gas scavenging enzymes were also isolated from human-host tissue samples, including skin lesions 91 , sputum [92][93][94][95] , lymph nodes 96 , the eye 97 , gastrointestinal tract 98 and haemodialysis water 99 . This is perhaps not surprising given that opportunistic pathogens within the Mycobacterium and Rhodococcus genera are capable of oxidising CO and/or H 2 at below atmospheric levels.…”

Section: Rubisco Form 1e Phylogeny Spans Eight Bacterial Phyla Inhabiting Environmental and Clinical Reservoirsmentioning

confidence: 99%

Atmospheric chemosynthesis is phylogenetically and geographically widespread and contributes significantly to carbon fixation throughout cold deserts

Ray

Zaugg

Benaud

et al. 2021

Preprint

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Cold desert soil microbiomes thrive despite severe moisture and nutrient limitations. In Eastern Antarctic soils, hydrogen oxidising bacteria support primary production through a novel carbon fixation process reliant on the chemoautotrophy-associated RuBisCO form IE. Here, biochemical assays show that atmospheric chemosynthesis occurs globally for primary production, contributing significantly to autotrophic carbon fixation throughout arid to hyperarid deserts in Antarctica, the high Arctic, and the Tibetan Plateau. Taxonomic and functional analyses were performed on 230 dereplicated medium-to-high quality metagenome-assembled genomes (MAGs) derived from 18 cold desert metagenomes and an additional 24,080 publicly available genomes. We infer that atmospheric chemosynthetic bacteria are widespread across environmental and clinical samples, increasing our knowledge of the bacterial phyla genetically capable of atmospheric chemosynthesis to seven, with key enzymes co-occurring within MAGs from four previously unidentified phyla; Chloroflexota, Firmicutes, Deinococcota and Verrucomicrobiota. We informatically identify an additional group of high-affinity hydrogenases, group 1m [NiFe]-hydrogenase using phylogenetics, gene structure analysis and homology modelling and reveal substantial new genetic diversity within RuBisCO form IE (rbcL1E), and high-affinity groups 1h and 1l [NiFe]-hydrogenases. Finally, we conclude that atmospheric chemosynthesis is a global phenomenon, extending throughout and beyond cold deserts, with significant implications for the global carbon cycle and bacterial survival within environmental and clinical reservoirs.

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Section: Rubisco Form 1e Phylogeny Spans Eight Bacterial Phyla Inhabiting Environmental and Clinical Reservoirsmentioning

confidence: 99%

Atmospheric chemosynthesis is phylogenetically and geographically widespread and contributes significantly to carbon fixation throughout cold deserts

Ray

Zaugg

Benaud

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…В рамках изучения особенностей генома нетуберкулезных микобактерий проведено полногеномное секвенирование ДНК трех штаммов нетуберкулезных микобактерий видов Mycobacterium gordonae, Mycobacterium malmoense и Mycobacterium heckeshornense, сборки геномов которых были депонированы в базу данных GenBank NCBI (Национальный центр биотехнологической информации, США) [19,20].…”

Section: микробиология и молекулярная генетика микобактериальных инфеunclassified

Tuberculosis in the Russian Federation: Situation, Challenges and Perspectives

Ergeshov¹

2018

Annals RAMS

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Tuberculosis (TB) is a significant problem of public health both in Russia and abroad. About one third of the world’s population is infected with Mycobacterium tuberculosis. Every year more than 10 million new TB cases are registered in the world, and about 1.7 million people die from TB. In the Russian Federation, due to the measures taken by the Government and health authorities the epidemic TB situation has been noticeably improved since the sharp deterioration in the 90s of the last century. At the same time, the spread of drug-resistant TB and its low treatment effectiveness, the spread of combined HIV and TB infection reduces the effectiveness of anti-tuberculosis interventions. The research conducted by CTRI, the WHO Collaborating Center for TB in the Russian Federation, is aimed at solving such urgent problems as studying latent infection mechanisms, developing new test systems for accelerated diagnostics of drug resistance, clinical approbation and introduction of short effective regimens of chemotherapy, developing new antituberculosis agents. The achieved successes in the study of tuberculosis in the 21st century creates preconditions for eliminating its epidemic both in Russia and the world, though suggesting imminent breakthrough for tuberculosis is a hasty conclusion.

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