First documented evidence of dengue and malaria co-infections in children attending two health centers in Yaoundé, Cameroon

Monamele, Gwladys Chavely; Demanou, Maurice

doi:10.11604/pamj.2018.29.227.15316

Cited by 32 publications

(43 citation statements)

References 6 publications

(6 reference statements)

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“…The frequency of dengue cases/malaria co-infections in our study was 13% (3/23) which is close to the 12.50% (3/24) observed in 2012/2013 in febrile patients in two hospitals of the capital city of Yaoundé, Cameroon [ 23 ]. Ayorinde et al .…”

Section: Discussionsupporting

confidence: 82%

“…It is the second study that reports cases of dengue/malaria co-infection after the one conducted from 2012 to 2013 in the city of Yaoundé, Cameroon after testing retrospectively malaria positive cases [ 23 ]. The objective of this study was to determine the frequency of detection of DENV, ZIKV and CHIKV acute infections in febrile patients consulting at the New-Bell District Hospital in Douala.…”

Section: Discussionmentioning

confidence: 99%

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Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon

et al. 2018

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Arboviruses are a major public health problem worldwide and are predominantly present in intertropical areas. Chikungunya, dengue and zika viruses have been implicated in recent epidemics in Asia, America and Africa. In Cameroon, data on these viruses are fragmentary. The purpose of this study was to determine the frequency of detection of these three viruses in febrile patients in Douala, Cameroon. A cross-sectional and descriptive study was conducted from March to April 2017 at the New-Bell District Hospital in Douala. Blood samples were collected from febrile patients and tested for malaria infections using Rapid Diagnostic test. Plasma harvested was later analyzed for the presence of chikungunya, dengue and zika viruses by a Trioplex real-time RT-PCR at Centre Pasteur of Cameroon. A total of 114 participants were included, of which 63.2% were females, reflecting a sex ratio (female/male) of 1.7. The median age was 26 years, range [0.25–81]. Eight (7%) of the 114 participants were infected with Dengue virus (DENV) among which 5 were identified as serotype 1. No cases of infection by either Zika virus or Chikungunya virus were detected. Three cases of dengue-malaria co-infection (13%) were recorded. No association was found between socio-demographic factors and dengue infection. The phylogenetic analysis of the partial envelope E gene showed that all the five DENV serotype 1 samples belonged to subtype V, similarly to strains from West African countries, particularly those from Nigeria, Senegal and Côte d’Ivoire. This study showed the circulation of DENV serotype 1 in febrile patients and raises the alarm for the establishment of a sustained surveillance system to detect cases and prevent potential outbreaks in Cameroon. The existence of dengue-malaria co-infections suggests that surveillance of arboviruses should not be limited to febrile, non-malarial cases.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon

et al. 2018

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“…Additionally, the presence of DENV-Ab immunocomplexes is likely to influence TLR2/CD14-mediated responses 60 . Lastly, considering the role of TLR2 and TLR4 in detecting bacterial PAMPS, it will be important to address the effect of common co-morbidities including bacterial and parasitic co-infections or microbial translocation 6,7,74,75 , on DENV pathogenesis and prognosis.…”

Section: Discussionmentioning

confidence: 99%

TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis

et al. 2020

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Vascular permeability and plasma leakage are immune-pathologies of severe dengue virus (DENV) infection, but the mechanisms underlying the exacerbated inflammation during DENV pathogenesis are unclear. Here, we demonstrate that TLR2, together with its coreceptors CD14 and TLR6, is an innate sensor of DENV particles inducing inflammatory cytokine expression and impairing vascular integrity in vitro. Blocking TLR2 prior to DENV infection in vitro abrogates NF-κB activation while CD14 and TLR6 block has a moderate effect. Moreover, TLR2 block prior to DENV infection of peripheral blood mononuclear cells prevents activation of human vascular endothelium, suggesting a potential role of the TLR2responses in vascular integrity. TLR2 expression on CD14 + + classical monocytes isolated in an acute phase from DENV-infected pediatric patients correlates with severe disease development. Altogether, these data identify a role for TLR2 in DENV infection and provide insights into the complex interaction between the virus and innate receptors that may underlie disease pathogenesis.

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“…During the past decade, there has been a rise of dengue cases in urban foci in Central Africa notably in Cameroon [26][27][28][29]. Even suspected, vectors were not well identified and characterised.…”

Section: Discussionmentioning

confidence: 99%

“…albopictus was identified as the main vector during concurrent dengue/chikungunya outbreak in Gabon in 2007 [8,22], and in Cameroon in 2006 [23]. During the last two decades, DENV-1 and DENV-2 mainly, were circulating in Cameroon [24][25][26][27][28][29]. Nationwide surveillance of dengue in 2006/2007 only revealed that seroprevalence (IgG and IgM antibodies) was higher in Douala [29].…”

Section: Introductionmentioning

confidence: 99%

Risk of dengue in Central Africa: Vector competence studies with Aedes aegypti and Aedes albopictus (Diptera: Culicidae) populations and dengue 2 virus

et al. 2019

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IntroductionDengue is the most important mosquito-borne diseases worldwide but was considered scarce in West-Central Africa. During the last decade, dengue outbreaks have increasingly been reported in urban foci in this region suggesting major epidemiological changes. However, in Central Africa where both vectors, Aedes aegypti and Aedes albopictus are well established, the role of each species in dengue transmission remains poorly investigated. Methodology/Principal findingsField-collected strains of Ae. aegypti and Ae. albopictus from different ecological settings in Central Africa were experimentally challenged with dengue 2 virus (DENV-2). Mosquitoes were analysed at 14-and 21-days post-infection. Analysis provide evidence that both Ae. aegypti and Ae. albopictus in Central Africa were able to transmit dengue virus with Ae. aegypti exhibiting a higher transmission rate. Unexpectedly, two Ae. aegypti populations from Bé noué and Maroua, in northern Cameroon, were not able to transmit DENV-2. Conclusions/SignificanceWe conclude that both Ae. aegypti and Ae. albopictus are susceptible to DENV-2 and may intervene as active dengue vectors. These findings highlight the urgent need to plan a vector surveillance program and control methods against dengue vectors in Central Africa in order to prevent future outbreaks. PLOS Neglected Tropical Diseases | https://doi.Dengue virus (DENV) is a flavivirus mainly transmitted to humans through the bite of infected mosquitoes notably Aedes aegypti and Aedes albopictus. In Central Africa where both vectors, Ae. aegypti and Ae. albopictus are well established, the role of each species in dengue transmission remains poorly investigated. Here, we assessed the vector competence of Ae. aegypti and Ae. albopictus collected in different ecological settings in Central Africa to transmit dengue 2 virus (DENV-2). We provide evidence that both Ae. aegypti and Ae. albopictus in Central Africa were able to transmit dengue virus with Ae. aegypti exhibiting a higher transmission rate. These findings could increase the risk of dengue outbreak in the region and emphasize the need for a comprehensive vector surveillance program to prevent and preparedness for an intervention in case of outbreaks.

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First documented evidence of dengue and malaria co-infections in children attending two health centers in Yaoundé, Cameroon

Cited by 32 publications

References 6 publications

Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon

Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon

TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis

Risk of dengue in Central Africa: Vector competence studies with Aedes aegypti and Aedes albopictus (Diptera: Culicidae) populations and dengue 2 virus

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