First development and characterization of 27 novel microsatellite markers in the dobsonfly Neoneuromus ignobilis (Megaloptera: Corydalidae) at genome-scale level

Phylogeography of the Oriental dobsonfly, Neoneuromus ignobilis Navás, suggests Pleistocene allopatric isolation and glacial dispersal shaping its wide distribution

“…For genotyping, we used the combination of two sets of molecular markers, that is, microsatellite markers and DNA sequence data from complete mitochondrial genomes. For the microsatellite markers, a total of 20 microsatellite loci previously developed by Lin et al (2020) were genotyped (Table S1). The PCR amplification program and sequencing method of the loci follow that described in Lin et al (2020).…”

“…For the microsatellite markers, a total of 20 microsatellite loci previously developed by Lin et al (2020) were genotyped (Table S1). The PCR amplification program and sequencing method of the loci follow that described in Lin et al (2020). Genotyping was conducted using the software GENEMAPPER v. 4.0 (Applied Biosystems, San Francisco, CA, U.S.A.).…”