First detection of Trypanosoma vivax in cattle in the state of Bahia, Brazil, based on parasitological and molecular analyses

Gomes, H. C. S. F.; Genipapeiro, Igor Leonardo de Jesus; Andrade, Fabiola; Barbosa, Danielle de Campos Vieira; Pacheco, Leonardo Ribas; Silva, Regiane Pereira Benevides; Patrocínio, Epaminondas do; Barbosa, C. de J.; Ribas, Jorge Raimundo Lins; Barbosa, Luciana Veiga

doi:10.5433/1679-0359.2021v42n3supl1p2065

Cited by 1 publication

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“…Despite the low seroprevalence, the parasite is dispersed in Minas Gerais State, which makes seronegative herds even more susceptible to the disease (Meneses, 2016). Other outbreaks of T. vivax in states bordering Minas Gerais, including São Paulo (Cadioli et al, 2012), Rio de Janeiro (Costa et al, 2020), Goiás (Bastos et al, 2020) and Bahia (Gomes et al, 2021) were also reported.…”

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confidence: 93%

Evaluation of techniques for diagnosis of Trypanosoma vivax infections in naturally infected cattle in the Zona da Mata Mineira

Alcindo

Vieira

Rocha

et al. 2022

Rev. Bras. Parasitol. Vet.

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This study aimed to evaluate diagnostic techniques for trypanosomiasis, caused by Trypanosoma vivax, in naturally infected cattle in Minas Gerais, Zona da Mata. The deaths of six lactating cows with similar clinical conditions—characterized by hyporexia, hypogalactia, and recumbency—had been reported from one property. Initially, two animals were examined and diagnosed with trypanosomiasis through identification of the protozoan in a blood smear. After the initial diagnosis, all lactating cows (n=37) on the property were examined, and blood samples were collected for tests including whole blood smear, buffy coat smear, Woo's technique, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). Woo's test, buffy coat smears, and whole blood smears indicated that 4/37 (10.81%) animals were positive for trypanosomiasis, whereas ELISA and PCR indicated that 33/37 (89.19%) and 27/37 (72.97%) animals, respectively, were positive. The agreement obtained between parasitological techniques was classified as high, while between ELISA and PCR, no agreement. In conclusion, parasitological techniques have a low capacity to identify infected animals in the chronic stage of T. vivax infection. Therefore, techniques such as PCR and/or ELISA should be used to minimize the occurrence of false negatives.

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