First Detection of the CTXM-15 Producing Escherichia coli O25-ST131 Pandemic Clone in Ecuador

Guacho, Carlos Vinicio Chiluisa; Escobar-Pérez, Javier; Dutra-Asensi, Marise

doi:10.3390/pathogens7020042

Cited by 11 publications

(5 citation statements)

References 5 publications

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“…The results showed that 100 lg/mL of the CcAgNps was more effective than 50 lg/mL, in inhibiting the biofilm formation, due to the presence of phytoconstituents and polyphenolic bioactive compound, which were capped and reduced by silver. It has been reported that green silver nanoparticles inhibited the biofilm formation by damaging the bacterial membrane structure and by disrupting quorum sensing [14]. CcAgNps acted as potential quorum quenching compounds which were due to the presence of phytochemical compounds of plant extract incorporated during nanoparticle synthesis [24].…”

Section: Resultsmentioning

confidence: 99%

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Green Nanocolloids Control Multi Drug Resistant Pathogenic Bacteria

Ranjani

Ahmed

Ruckmani³

et al. 2019

J Clust Sci

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Cymbopogon citratus is grown globally for therapeutic application is utilized to synthesize colloidal silver nanoparticles (CcAgNps). CcAgNps are characterized by biophysical methods and are tested against CTXM-15 expressing pathogenic Escherichia coli strains. MIC, MBC and biofilm assays are performed at different concentrations of nanoparticles. The expression of CTXM-15 gene responsible for antibiotic resistance is analyzed by polymerase chain reaction to identify the role of CTXM-15 in pathogenesis. CcAgNps show better antibacterial and anti-biofilm activity when compared to antibiotics. This study suggests that CcAgNps are the potential antibiotic resistance breakers to control pathogenic E. coli and can be utilized as alternatives to antibiotics.

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Section: Resultsmentioning

confidence: 99%

“…Biophysically characterized colloidal CcAgNps were used to test the efficacy on CTXM-15 positive E. coli and its CTXM-15 gene expression studies. Because ''The CTX-M pandemic'' is E. coli producing CTXM enzyme emerged globally due to onset of UTI's [14]. The most widely distributed is CTXM-15 enzyme which was detected in 2001.…”

Section: Introductionmentioning

confidence: 99%

Green Nanocolloids Control Multi Drug Resistant Pathogenic Bacteria

Ranjani

Ahmed

Ruckmani³

et al. 2019

J Clust Sci

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“…Escherichia coli is the main ESBL‐producing bacterium identified in food products, human and environmental samples worldwide (Rawat and Nair ; Ojer‐usoz et al ). In Ecuador, ESBL‐producing E. coli strains have been characterized in clinical samples, raw vegetables, dog faeces and broiler chickens (Gestal et al ; Ortega‐Paredes et al ; Chiluisa‐Guacho et al ; Soria Segarra et al ; Hedman et al ; Vinueza et al ; Zurita et al ). However, there is no data about the contamination of street food with ESBL‐producing E. coli strains.…”

Section: Introductionmentioning

confidence: 99%

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Zurita

Yánez

Sevillano³

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: This study identified ESBL-producing Escherichia coli epidemic clones: ST131, ST410 and ST744 in ready-to-eat street food samples. Street food is a possible way to spread harm multidrug-resistant (MDR) E. coli strains in the community. Studies to identify the contamination sources of this kind of food are needed to tackle MDR E. coli dissemination. AbstractReady-to-eat food contamination with ESBL-producing Escherichia coli is a growing health concern. Some of these strains also are epidemic clones and can cause community-associated infections that are difficult to treat. In this study, the occurrence of ESBL-producing E. coli contaminated ready-to-eat street food in Quito, Ecuador was evaluated. In total, 150 samples were collected randomly in the most crowded sites of the city. In all, 34 samples (34/150; 22Á6%) were positive for total thermotolerant (44Á5°C) coliforms resistant to cefotaxime. MALDI-TOF analysis identified that the E. coli was found in 20 food samples (20/34; 59%). ESBL gene bla CTX-M-55 was identified in nine isolates, bla CTX-M-15 in six isolates, bla CTX-M-14 in two isolates, and one isolate each harboured bla CTX-M-24 , bla CTX-M-65 , bla CTX-M-55 and bla CTX-M-8 . Phylogenetic groups like A and B1 were the most common, followed by groups D and B2. MLST analysis identified 12 different sequence types (STs), the most common was ST162. Recognized epidemic clonal groups ST410, ST131 and ST744 were encountered. Ready-to-eat street food is a potential way of spreading ESBL-producing E. coli epidemic clones in Quito, Ecuador.Letters in Applied Microbiology ISSN 0266-8254

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“…Recent Latin American studies have shown an increase in the prevalence of this group of enzymes in E. coli isolates [ 34 , 35 , 36 , 37 ]. In Latin America, clinical isolate studies have been carried out where the dissemination of the ST43/ST131 clone is evidenced in several countries both at the hospital and community level [ 17 , 23 , 24 ], however, in the Central American region there is little data regarding the identification of this clone. Studies have shown a prevalence of E. coli ST131 among non-ESBL-producing isolates of between 10 and 13% [ 8 ], however, in our analyzed strains, all strains belonging to this clone were carriers of ESBL.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies from Latin American countries have described the presence of ST131, both in hospitals and the community [ 19 , 20 , 21 , 22 ]. In Ecuador, a study of E. coli serotype O25 carrying ESBL belonged to ST 131/PST43 and resulted in carriers of CTX-M-15 [ 23 ]. However, to the best of our knowledge, there are still no reports on the molecular epidemiology of infection-causing E. coli strains in Central America [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Escherichia coli Clinical Isolates from Central Panama

Núñez-Samudio

Pecchio²,

Pimentel-Peralta³

et al. 2021

Antibiotics

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Escherichia coli represents one of the most common causes of community-onset and nosocomial infections. Strains carrying extended spectrum β-lactamases (ESBL) are a serious public health problem. In Central America we have not found studies reporting the molecular epidemiology of E. coli strains implicated in local infections, so we conducted this study to fill that gap. Materials and Methods: We report on an epidemiological study in two reference hospitals from central Panama, identifying the susceptibility profile, associated risk factors, and molecular typing of E. coli strains isolated between November 2018 and November 2019 using Pasteur’s Multilocus Sequence Typing (MLST) scheme. Results: A total of 30 E. coli isolates with antimicrobial resistance were analyzed, 70% of which came from inpatients and 30% from outpatients (p < 0.001). Two-thirds of the samples came from urine cultures. Forty-three percent of the strains were ESBL producers and 77% were resistant to ciprofloxacin. We identified 10 different sequence types (STs) with 30% of the ESBL strains identified as ST43, which corresponds to ST131 of the Achtman MLST scheme—the E. coli pandemic clone. Thirty-eight percent of the E. coli strains with the ESBL phenotype carried CTX-M-15. Conclusions: To the best of our knowledge, this is the first report confirming the presence of the pandemic E. coli clone ST43/ST131 harboring CTX-M-15 in Central American inpatients and outpatients. This E. coli strain is an important antimicrobial-resistant organism of public health concern, with potential challenges to treat infections in Panama and, perhaps, the rest of Central America.

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First Detection of the CTXM-15 Producing Escherichia coli O25-ST131 Pandemic Clone in Ecuador

Cited by 11 publications

References 5 publications

Green Nanocolloids Control Multi Drug Resistant Pathogenic Bacteria

Green Nanocolloids Control Multi Drug Resistant Pathogenic Bacteria

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Molecular Epidemiology of Escherichia coli Clinical Isolates from Central Panama

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