First detection of SARS-CoV-2 genetic material in the vicinity of COVID-19 isolation centre through wastewater surveillance in Bangladesh

Abstract

In the course of a COVID-19 pandemic, 0.33 million people got infected in Bangladesh, we made the first and successful attempt to detect SARS-CoV-2 viruses' genetic material in the vicinity wastewaters of an isolation centre i.e. Shaheed Bhulu Stadium, situated at Noakhali. The idea was to understand the genetic loading variation, both temporal and distance-wise in the nearby wastewater drains when the number of infected COVID-19 patients is not varying much. Owing to the fact that isolation center, in general, always contained a constant number of 200 COVID-19 patients, the prime objective of the study was to check if several drains carrying RNA of coronavirus are actually getting diluted or accumulated along with the sewage network. Our finding suggested that while the temporal variation of the genetic load decreased in small drains over the span of 50 days, the main sewer exhibited accumulation of SARS-CoV-2 RNA. Other interesting finding displays that probably distance of sampling location in meters is not likely to have a significant impact on gene detection concentration, although the quantity of the RNA extracted in the downstream of the drain was higher. These findings are of immense value from the perspective of wastewater surveillance of COVID-19, as they largely imply that we do not need to monitor every wastewater system, and probably major drains monitoring may illustrate the city health. Perhaps, we are reporting the accumulation of SARS-CoV-2 genetic material along with the sewer network i.e. from primary to tertiary drains. The study sought further data collection in this line to simulate conditions prevailed in the most of south Asian country and to shed further light on the temporal variation and decay/accumulation processes of the genetic load of the SARS-COV-2.

Association of household fuel with acute respiratory infection (ARI) under-five years children in Bangladesh

“…Human respiratory-related infections (RTIs) are known as the most familiar human infections, while ARIs are found in the upper and lower side of respiratory tract. The population compactness of Bangladesh is 1,265 sq/km with a total of 165 million as of 04 July 2022 ( 56 , 59 ). Of the study households, solid fuel (i.e., coal/lignite, charcoal, wood, straw/shrubs/grass, agricultural crops, and animal dung) is the most widely used fuel for cooking in Bangladesh.…”

Wastewater-Based Epidemiology as an Early Warning System for the Spreading of SARS-CoV-2 and Its Mutations in the Population

“…The most frequently used primers target N genes, which are also recommended by the CDC [ 77 ]. RNA SARS-CoV-2 was detected in wastewater worldwide such as in Europe [ 21 , 23 , 31 , 32 , 41 , 50 , 52 , 74 ], Asia [ 32 , 55 , 71 , 72 , 73 , 75 ], America [ 48 , 76 ], and Australia [ 49 ].…”

The Urban Water Cycle as a Planning Tool to Monitor SARS-CoV-2: A Review of the Literature

“…[156], Kuryntseva et al [123], Fangaro et al [59], Betancourt et al [161] and Colosi et al [155], among others, have proposed epidemiological models and analyses of growth rates according to the study area and temporalities. These proposed models and methodologies have made it possible to understand the continuity, behaviour and growth of the virus in a given population [56,193,194]. Additionally, this kind of study provides reliable information on the behaviour of the virus in asymptomatic patients and allows researchers to determine the number of undiagnosed infections in a population [192].…”