First detection of Leishmania infantum kinetoplast DNA in hair of wild mammals: Application of qPCR method to determine potential parasite reservoirs

Muñoz-Madrid, Rubén; Belinchón-Lorenzo, Silvia; Iniesta, Virginia; Fernández-Cotrina, Javier; Parejo, Juan Carlos; Serrano, Francisco; Monroy, Isabel; Baz, Victora; Gómez-Luque, Adela; Gómez-Nieto, Luis Carlos

doi:10.1016/j.actatropica.2013.08.009

Cited by 43 publications

(45 citation statements)

References 16 publications

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“…There is still no general consensus regarding the standard sample of choice Lombardo et al, 2012); however, the use of non-invasive sampling techniques could serve to simplify diagnostic procedures, epidemiological studies, field trials, reduce pain and thus would be more readily accepted by owners. The noninvasive samples tested so far have been urine, hair, CS, OS, nasal and ear swabs Carvalho Ferreira et al, 2014;Ferreira et al, 2013;Gramiccia et al, 2010;Lombardo et al, 2012;Manna et al, 2008;Muñoz-Madrid et al, 2013;Solano-Gallego et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Hernández

Montoya

Checa

et al. 2015

Veterinary Parasitology

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Section: Introductionmentioning

confidence: 99%

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Hernández

Montoya

Checa

et al. 2015

Veterinary Parasitology

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“…Human and animal leishmaniases. Leishmania DNA has been detected and quantified in the cerumen of infected dogs [342]. A recent publication demonstrates that cerumen-qPCR expresses the highest sensitivity (87.5%) to detect genetic materials, followed by hair (lesions: 78.57%, healthy skin: 62.5%), and blood (68.75%) [343].…”

Section: Ear Swab/cerumenmentioning

confidence: 99%

“…Therefore, with this methodology, it is not the infectious agent that is detected, nor the immunologic response, nor the set of volatile compounds exhaled by the dogs. Although hair is not known as a target tissue for Leishmania, an investigation of Leishmania DNA was undertaken on CVL in a mouse experimental model of infection with L. major but also in the hair of wild mammals or Leporidae [342,349,350]. In mice, the DNA of L. major is detected near the inoculation site but also in hair collected in body areas far from the infection site [349].…”

Section: Hair/bristlesmentioning

confidence: 99%

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Sereno

Akhoundi

Sayehmri

et al. 2020

IJMS

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Unicellular eukaryotes of the Trypanosomatidae family include human and animal pathogens that belong to the Trypanosoma and Leishmania genera. Diagnosis of the diseases they cause requires the sampling of body fluids (e.g., blood, lymph, peritoneal fluid, cerebrospinal fluid) or organ biopsies (e.g., bone marrow, spleen), which are mostly obtained through invasive methods. Body fluids or appendages can be alternatives to these invasive biopsies but appropriateness remains poorly studied. To further address this question, we perform a systematic review on clues evidencing the presence of parasites, genetic material, antibodies, and antigens in body secretions, appendages, or the organs or proximal tissues that produce these materials. Paper selection was based on searches in PubMed, Web of Science, WorldWideScience, SciELO, Embase, and Google. The information of each selected article (n = 333) was classified into different sections and data were extracted from 77 papers. The presence of Trypanosomatidae parasites has been tracked in most of organs or proximal tissues that produce body secretions or appendages, in naturally or experimentally infected hosts. The meta-analysis highlights the paucity of studies on human African trypanosomiasis and an absence on animal trypanosomiasis. Among the collected data high heterogeneity in terms of the I 2 statistic (100%) is recorded. A high positivity is recorded for antibody and genetic material detection in urine of patients and dogs suffering leishmaniasis, and of antigens for leishmaniasis and Chagas disease. Data on conjunctival swabs can be analyzed with molecular methods solely for dogs suffering canine visceral leishmaniasis. Saliva and hair/bristles showed a pretty good positivity that support their potential to be used for leishmaniasis diagnosis. In conclusion, our study pinpoints significant gaps that need to be filled in order to properly address the interest of body secretion and hair or bristles for the diagnosis of infections caused by Leishmania and by other Trypanosomatidae parasites.

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“…The proteinase K method, which was used to isolate hair DNA, is one of the most common methods employed for this type of templates Muñoz-Madrid et al, 2013;Pfeiffer et al, 2004). The obtained DNA amount ranging from 108 to 144 ng (in 20 µl of extract) was objectively small, but much higher than reported in the literature; by way of comparison, the literature on the subject mentions that 0.4 ng was extracted from an elephant's hair (Heywood et al, 2003).…”

Section: Volmentioning

confidence: 99%

Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces

Natonek-Wiśniewska

Krzyscin

2017

Acta Biochim Pol

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The objective of the study was to demonstrate how mitochondrial DNA (mtDNA) can be used to determine the species origin of animal microtraces. The study included pieces of cat and dog hair without the root, a fragment of cooked chicken bone (0.1g), three goose down samples (0.028 g), a pork swab, a pork scratching (5×5×5 mm), and pork lard (0.22 g). DNA was isolated from all of these samples using the method appropriate for the particular source material. The extracts had DNA concentration exceeding 5.4 ng/µl with A purity range of 1.14-1.88. Next, the samples were subjected to PCR and real-time PCR with species-specific primers and primers complementary to mitochondrial DNA (mtDNA). Control reactions based on the amplification of eukaryotic-specific fragment (18S rRNA) were additionally performed. PCR and real-time PCR products for detection of species-specific mtDNA were obtained for all templates, whereas during the detection of eukaryote DNA no product was obtained for dog and cat hair only. The poor quality of the obtained DNA did not prevent the analysis. The results showed that mitochondrial DNA is suitable for identification of small or highly processed samples, in which genomic DNA often cannot be analyzed.

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First detection of Leishmania infantum kinetoplast DNA in hair of wild mammals: Application of qPCR method to determine potential parasite reservoirs

Cited by 43 publications

References 16 publications

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces

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