First detection of hepatitis E virus in Central Argentina: Environmental and serological survey

Wassaf, Maribel Graciela Martínez; Pisano, María Belén; Barril, Patricia A.; Elbarcha, Osvaldo; Pinto, Marcelo Alves; Oliveira, Jaqueline Mendes de; DiGiusto, Pablo; Nates, Silvia; Ré, Viviana Elizabeth

doi:10.1016/j.jcv.2014.08.016

Cited by 59 publications

(51 citation statements)

References 39 publications

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“…Wastewater samples obtained in second and third samplings were divided in 50 mL and 1500 mL aliquots: the 50 mL aliquots were concentrated by flocculation with skimmed milk as described by Calgua et al [43,44] and the 1500 mL aliquots were concentrated by modified PEG technique described by Martinez Wassaf et al [11,45] (Fig 2). The viral concentrates were stored at—70°C.…”

Section: Methodsmentioning

confidence: 99%

“…Indeed, the presence of HEV has been demonstrated in several studies performed in environmental water, river and wastewater samples in America, Asia and Europe [11–15]. …”

Section: Introductionmentioning

confidence: 99%

“…Noteworthy, HEV genotype 3 was recently identified in river and sewage samples in Argentina [11]. Additionally, genotype 1 has been found in human samples from Cuba, Mexico, Uruguay and Venezuela, while genotype 2 has only been described in outbreaks in Mexico between 1986 and 1987 [21,25–28].…”

Section: Introductionmentioning

confidence: 99%

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First evidence of the Hepatitis E virus in environmental waters in Colombia

et al. 2017

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Hepatitis E virus (HEV) is one of the main causes of acute viral hepatitis of enteric transmission. HEV has been detected in environmental samples in several countries from Europe and Asia, constituting a risk factor for waterborne infection. In Colombia, HEV has been identified in samples obtained from patients as well as from swine, but no environmental studies have been carried out. To determine if HEV is present in environmental waters, samples from the main source of drinking water plant and of wastewater system of eight municipalities and two villages of Antioquia state (North West Colombia), were collected between December 2012 and April 2014. The HEV genome was detected by RT-PCR in 23.3% (7/30) of the samples from the main source of drinking water plants and in 16.7% (5/30) from sewage. Viral concentrates obtained from three positive sewage samples were used to inoculate HepG2 cell cultures that were followed for one month; however, the viral genome was not detected in any cell culture. This study demonstrates the circulation of HEV in both source of drinking water plants and wastewater in Antioquia state, Colombia. The presence of HEV in environmental waters could be a risk for waterborne transmission in this population. The findings of the present study, together with the evidence of HEV circulation in human and swine in Colombia, should be consider by public health authorities for the development of surveillance programs and the inclusion of HEV infection diagnosis in the guidelines of viral hepatitis in the country. This is the first report of HEV in environmental samples in Colombia and the second one in Latin America.

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Section: Methodsmentioning

confidence: 99%

“…Indeed, the presence of HEV has been demonstrated in several studies performed in environmental water, river and wastewater samples in America, Asia and Europe [11–15]. …”

Section: Introductionmentioning

confidence: 99%

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First evidence of the Hepatitis E virus in environmental waters in Colombia

et al. 2017

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“…In this sense, the implementation of more robust technologies is required for an easier and more reliable detection of this virus. Previous local reports used conventional nested PCRs as HEV detection tool (Martinez Wassaf et al, ; Pisano, Lugo, et al, ). However, a high variability in the results was observed with this molecular reaction, depending on the ORF targeted for amplification (Martinez Wassaf et al, ; Mirazo, Ramos, Russi, & Arbiza, ).…”

Section: Introductionmentioning

confidence: 99%

Detection of HEV in naturally infected swine from central Argentina by an optimized HEV/MS2 duplex RT‐qPCR

Marziali

Acosta

Bolatti

et al. 2019

Zoonoses and Public Health

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Hepatitis E virus (HEV) is currently considered as a global health concern due to the recognition of its zoonotic transmission to humans, mainly from swine, and its association with the development of severe cases of hepatitis in human risk populations. The lack of updated data on HEV state of infection in swineherds of Argentina, and the necessity of robust technologies for its detection in complex biological samples, positions HEV as an emerging issue in public health. Here, we have optimized a RT‐qPCR with internal control for a more precise and accurate HEV RNA detection in swine stool samples. We implemented this optimized molecular tool to analyse the current epidemiological scenario of HEV infection in swine from the core region of commercial activity of Argentina. A total of 135 stool samples were collected from 16 different farms and tested for HEV presence, resulting in 11 positive cases (8.1%). Phylogenetic analysis demonstrated that all of them correspond to HEV genotype 3 and that different subtypes circulate in the region. Moreover, two of the detected strains presented a high nucleotide similarity with a previously identified isolate from human sewage discharges, suggesting the zoonotic transmission of HEV to humans. Collectively, this work provides a better understanding of HEV epidemiology in Argentina while contributes to the improvement of HEV detection technologies.

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“…Las muestras de agua residual del primer muestreo se concentraron mediante la técnica de polietilenglicol descrita por Sobsey (34) con las modificaciones introducidas en el protocolo por González, et al (35). Para el segundo y el tercer muestreos, se utilizaron las técnicas de polietilenglicol descritas por Martínez, et al (36), y la de floculación con leche descremada descrita por Calgua, et al (37,38). Los productos de la concentración viral se almacenaron a -70 °C hasta su posterior análisis.…”

Section: Concentración De Partículas Viralesunclassified

Evidencia de circulación del virus de la hepatitis A, subgenotipo IA, en muestras ambientales en Antioquia, Colombia

et al. 2016

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Introducción. El virus de la hepatitis A (HAV) es un importante patógeno que se transmite por vía fecal-oral. La epidemiología de la infección está directamente relacionada con el acceso de la población al agua potable y con la infraestructura de alcantarillado. Objetivo. Determinar la presencia del HAV e identificar el genotipo en muestras de agua de abastecimiento y agua residual en ocho municipios, un corregimiento y una vereda del departamento de Antioquia, noroccidente de Colombia. Materiales y métodos. Se hicieron tres muestreos seriados de diciembre de 2012 a abril de 2014 en la fuente principal de abastecimiento de los acueductos y en el principal vertimiento de aguas residuales de cada municipio. Las muestras se concentraron por filtración y ultrafiltración tangencial, y por las técnicas de polietilenglicol y floculación con leche descremada, respectivamente. A partir del ARN total de cada muestra, se amplificaron la región VP3-VP1 para la detección del genoma viral y la región VP1-2B para la genotipificación. Resultados. El genoma del HAV se detectó en las fuentes de agua de abastecimiento de Puerto Berrío, Frontino y Nutibara, y en las muestras de aguas residuales provenientes de los municipios de Arboletes, Zaragoza y Venecia. Mediante el análisis de las secuencias se identificó el subgenotipo IA del virus. Conclusión. Este estudio permitió detectar la presencia del HAV en 6,6 % de las muestras de agua de abastecimiento y en 13,3 % de las muestras de agua residual de los municipios en estudio. Se reporta por primera vez la circulación del subgenotipo IA en muestras ambientales en Antioquia.

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First detection of hepatitis E virus in Central Argentina: Environmental and serological survey

Cited by 59 publications

References 39 publications

First evidence of the Hepatitis E virus in environmental waters in Colombia

First evidence of the Hepatitis E virus in environmental waters in Colombia

Detection of HEV in naturally infected swine from central Argentina by an optimized HEV/MS2 duplex RT‐qPCR

Evidencia de circulación del virus de la hepatitis A, subgenotipo IA, en muestras ambientales en Antioquia, Colombia

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