First detection of bovine group B rotavirus in Japan and sequence of its VP7 gene

Abstract

An epizootic outbreak of diarrhea occurred in adult cows on a dairy farm in Hokkaido, Japan. One colostrum-fed calf inoculated with pooled feces of the 5 affected cows, developed mild diarrhea, and shed rotavirus-like particles which reacted with antiserum to group B rotavirus in immune electron microscopy. Cell culture immunofluorescence tests, RNA-polyacrylamide gel electrophoresis and RT-PCR confirmed that this virus was bovine group B rotavirus, which was designated the Nemuro strain. Additional 2 colostrum-deprived calves inoculated with feces of the first calf also developed diarrhea and shed virus, suggesting that this group B rotavirus might be the etiological agent of the outbreak of adult cow diarrhea. The identities of the nucleotide (nt) and deduced amino acid (aa) sequences of the Nemuro VP7 gene were high (93-95% in nt and 96-97% in aa) and low (61-63% in nt and 49-61% in aa) compared to those of the published corresponding genes from 3 bovine and 2 other mammalian (human and rat) strains of group B rotaviruses, respectively. To our knowledge, this is the first report on the presence of bovine group B rotavirus in Japan.

Detection of a Bovine Group C Rotavirus from Adult Cows with Diarrhea and Reduced Milk Production

“…In contrast, non-lactating young cattle at the same farm did not show diarrhea. These characteristics are closely similar to those of BCV infection [15] and also bovine GBR infection [6,22], which in turn indicates the possibility that GCR infection may have been overlooked clinically in adult cow diarrhea. Thus, laboratory diagnosis including GCR-testing is needed to define the etiological role of GCR infection in adult cow diarrhea.…”

“…For GCR detection by RT-PCR, the full-length VP7 gene was amplified with a primer pair corresponding to the 5' and 3' ends of strain Shintoku VP7 gene [20]. RT-PCR for GAR, group B rotavirus (GBR), bovine viral diarrhea virus (BVDV) and bovine coronavirus (BCV) detections were performed as described previously [5,22,24,25]. For amplification of the full-length VP6 gene of GCR, a primer pair corresponding to the common 5' and 3' ends of VP6 gene was used [9].…”

Human Group B Rotavirus Infections Cause Severe Diarrhea in Children and Adults in Bangladesh

“…201 to 550 of the adult diarrheal rotavirus (ADRV) sequence (7) was determined for specimen numbers 334, 373, and 402. This sequence of the three viruses was identical and showed high sequence identity to the same sequence regions of human group B rotaviruses CAL-1 (13) (99%) and ADRV (92%), whereas the identities with those of bovine virus WD653 (26) and murine virus IDIR (20) were considerably low (62 and 56%, respectively) (data not shown). The partial NSP2 gene (gene 8) sequence (nucleotide no.…”

Sequencing and Sequence Analysis of VP7 and NSP5 Genes Reveal Emergence of a New Genotype of Bovine Group B Rotaviruses in India

“…Bovine GBRs have been reported only from the United States, the United Kingdom, and Japan (2,3,6,24). Recently, an extensive review on molecular characterization and epidemiology of GBRs has been written by Sen et al (20).…”

“…The amplified PCR products were 795 bp long (partial) for VP7 and 625 bp long (full length) for NSP5 in all three strains ( Due to lack of tissue culture adaptation of GBRs, except for one porcine strain (16), no serotyping scheme has been defined for GBRs at present (13). Moreover, only a few GBR VP7 genes have been analyzed and compared (2,4,11,14,20,24) to develop a systemic genotyping scheme for GBRs. The VP7 gene sequences of all four published bovine GBR strains showed significantly high identity (92 to 97%) among themselves, and they may belong to the same genotype (24).…”