First detection of Borrelia miyamotoi infections in ticks and humans from the northeast of Inner Mongolia, China

Gao, Yuan; Lv, Xiaolong; Han, Shu-Zheng; Wang, Wei; Liu, Quan; Song, Mingxin

doi:10.1016/j.actatropica.2021.105857

Cited by 20 publications

(29 citation statements)

References 31 publications

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“…We conducted large-scale tick surveillance for B. miyamotoi in Greater Khingan, as human cases of B. miyamotoi infection were confirmed in Jilin and Heilongjiang, China, in 2018, and B. miyamotoi has previously been found in I. persulcatus ticks [ 12 ]. From our research, the prevalence of B. miyamotoi among I. persulcatus was shown to be approximately 5%, which is similar to the prevalence shown by the most recent study in China (approximately 3% in I. persulcatus ) [ 12 , 38 ]. Most previous reports suggest that Ixodes ticks are transmission vectors of B. miyamotoi in North America, Europe, and in other Asian countries.…”

Section: Discussionsupporting

confidence: 87%

“…Before this study, no isolates of this spirochete from China had been cultured, and there was little information on B. miyamotoi infections in humans and ticks [12,37,38]. In this study, we detected B. miyamotoi in I. persulcatus ticks and successfully isolated a B. miyamotoi strain [12,37,38], the potential for Haemaphysalis and Dermacentor ticks to act as vectors of B. miyamotoi remains unclear. Haemaphysalis, however, is suggested to be a vector of Borrelia species related to B. theileri in Japan [39,40].…”

Section: Discussionmentioning

confidence: 89%

“…Borrelia miyamotoi is a newly described emerging pathogen. Before this study, no isolates of this spirochete from China had been cultured, and there was little information on B. miyamotoi infections in humans and ticks [ 12 , 37 , 38 ]. In this study, we detected B. miyamotoi in I. persulcatus ticks and successfully isolated a B. miyamotoi strain from I. persulcatus collected in Greater Khingan, Inner Mongolia, China.…”

Section: Discussionmentioning

confidence: 99%

“…While other tick species ( Haemaphysalis and Dermacentor ticks) have been found to carry B. miyamotoi in China [ 12 , 37 , 38 ], the potential for Haemaphysalis and Dermacentor ticks to act as vectors of B. miyamotoi remains unclear. Haemaphysalis , however, is suggested to be a vector of Borrelia species related to B. theileri in Japan [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

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Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

et al. 2021

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Background Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks, and we aimed to isolateB. miyamotoi from I. persulcatus from four regions of Greater Khingan, Inner Mongolia, China. Methods From May to June each year during the period 2016–2019, host-seeking adult I. persulcatus ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and infections with other borreliae. For characterization of the isolated B. miyamotoi, we performed draft genome sequencing and multilocus sequencing analysis (MLSA). Results A total of 2656 adult I. persulcatus ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as B. miyamotoi. Furthermore, the B. miyamotoi strain Hetao-1 was isolated from I. persulcatus, and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia. Conclusion We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate from China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

et al. 2021

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“…Since 2011, when the first symptomatic case of human infection was recorded in Russia, B. miyamotoi has been classified as a human pathogen [ 22 ]. Further cases of human infection were reported in the USA [ 23 , 24 ], Europe (the Netherlands, Germany, Poland, Sweden, and France) [ 25 , 26 , 27 , 28 ], and Asia [ 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Abundance of Ixodes ricinus Ticks (Acari: Ixodidae) and the Diversity of Borrelia Species in Northeastern Poland

Kubiak

Szymańska

Dmitryjuk

et al. 2022

IJERPH

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Monitoring the abundance of ticks and the prevalence of pathogens in ticks is an important activity in assessing the risk of tick-borne diseases and helps to develop preventive measures. This study aimed to estimate the density of Ixodes ricinus, the prevalence of Borrelia species, and their diversity in northeastern Poland. The overall mean I. ricinus density was 9.7 ticks/100 m2. There were no differences between years, subregions, or habitats of study. The Borrelia infection rate was higher in females (22.6%) and males (14.3%) than in nymphs 5.5% (MIR). The most infected ticks came from the eastern subregion (10.1%) where the incidence of borreliosis among the inhabitants was over 20% higher than in the other subregions. In the infected ticks, B. afzelii (38.3%) and B. garinii (34.5%) were predominant. B. bavariensis was confirmed in I. ricinus in Poland for the first time. The most polymorphic was B. garinii. B. miyamotoi (belonged to the European type) was identified as a mono-infection in 0.9% of ticks and in 1.5% as a co-infection with B. afzelii and with B. garinii. Besides the risk of borreliosis and co-infections with different Borrelia species, physicians should also be aware of B. miyamotoi infections among patients.

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Spatial risk of Haemaphysalis longicornis borne Dabieshan tick virus (DBTV) in China

Han,

Gao,

Xin

et al. 2024

Journal of Medical Virology

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The uncertainty and unknowability of emerging infectious diseases have caused many major public health and security incidents in recent years. As a new tick‐borne disease, Dabieshan tick virus (DBTV) necessitate systematic epidemiological and spatial distribution analysis. In this study, tick samples from Liaoning Province were collected and used to evaluate distribution of DBTV in ticks. Outbreak points of DBTV and the records of the vector Haemaphysalis longicornis in China were collected and used to establish a prediction model using niche model combined with environmental factors. We found that H. longicornis and DBTV were widely distributed in Liaoning Province. The risk analysis results showed that the DBTV in the eastern provinces of China has a high risk, and the risk is greatly influenced by elevation, land cover, and meteorological factors. The risk geographical area predicted by the model is significantly larger than the detected positive areas, indicating that the etiological survey is seriously insufficient. This study provided molecular and important epidemiological evidence for etiological ecology of DBTV. The predicted high‐risk areas indicated the insufficient monitoring and risk evaluation and the necessity of future monitoring and control work.

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First detection of Borrelia miyamotoi infections in ticks and humans from the northeast of Inner Mongolia, China

Cited by 20 publications

References 31 publications

Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

Abundance of Ixodes ricinus Ticks (Acari: Ixodidae) and the Diversity of Borrelia Species in Northeastern Poland

Spatial risk of Haemaphysalis longicornis borne Dabieshan tick virus (DBTV) in China

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