First detection of a plasmid located carbapenem resistant blaVIM-1 gene in E. coli isolated from meat products at retail in Belgium in 2015

Garcia-Graells, Cristina; Berbers, Bas; Verhaegen, Bavo; Vanneste, Kevin; Marchal, Kathleen; Roosens, Nancy; Botteldoorn, Nadine; Keersmaecker, Sigrid C. J. De

doi:10.1016/j.ijfoodmicro.2020.108624

Cited by 17 publications

(15 citation statements)

References 33 publications

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“…This bla VIM-1 harboring E. coli isolate 19-AB01133 and its comprehensive molecular characteristics are described in this study. It shows great similarities to an E. coli isolate described by Garcia-Graells et al (2020) [16] that was isolated from a pork sample in Belgium. Both isolates harbored almost identical IncA/C 2 plasmids, which encode the bla VIM-1 gene.…”

Section: Introductionsupporting

confidence: 72%

“…No. MN477204.1) isolated from minced pork in Belgium in 2015 [16]. The corresponding isolate was assigned to the same sequence type, which might indicate a clonal relationship.…”

Section: Resultsmentioning

confidence: 96%

“…The encoded bla VIM-1 gene is highly likely to be responsible for the resistance to the tested carbapenems (imipenem, ertapenem and meropenem) in the E. coli isolate 19-AB01133. To the best of our knowledge, bla VIM-1 -carrying IncA/C 2 plasmids were detected sporadically in human samples [22,23], but so far only once in livestock and food in Europe [16]. Recently, Garcia-Grealls and her colleagues (2020) described an almost identical VIM-1 encoding plasmid (99.99% identical over the entire plasmid nucleotide sequence, Acc.…”

Section: Resultsmentioning

confidence: 98%

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Identification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

et al. 2020

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Within the German national monitoring of zoonotic agents, antimicrobial resistance determination also targets carbapenemase-producing (CP) Escherichia coli by selective isolation from food and livestock. In this monitoring in 2019, the CP E. coli 19-AB01133 was recovered from pork shoulder. The isolate was assigned to the phylogenetic group B1 and exhibited the multi-locus sequence-type ST5869. Molecular investigations, including whole genome sequencing, of 19-AB01133 revealed that the isolate carried the resistance genes blaVIM-1, blaSHV-5 and blaCMY-13 on a self-transmissible IncA/C2 plasmid. The plasmid was closely related to the previously described VIM-1-encoding plasmid S15FP06257_p from E. coli of pork origin in Belgium. Our results indicate an occasional spread of the blaVIM-1 gene in Enterobacteriaceae of the European pig population. Moreover, the blaVIM-1 located on an IncA/C2 plasmid supports the presumption of a new, probably human source of carbapenemase-producing Enterobacteriaceae (CPE) entering the livestock and food chain sector.

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Section: Introductionsupporting

confidence: 72%

“…No. MN477204.1) isolated from minced pork in Belgium in 2015 [16]. The corresponding isolate was assigned to the same sequence type, which might indicate a clonal relationship.…”

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 98%

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Identification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

et al. 2020

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“…Resistance to mecillinam and imipenem was not observed in our study. Sporadic cases of ESBL-producing E. coli resistance to imipenem (carbapenems) obtained from pigs or pork in the EU have been reported in Germany [ 4 ] and Belgium [ 30 ]. Mecillinam belongs to the group of broad-spectrum penicillins and historically it has not been widely used in veterinary medicine, possibly due to its poor oral absorption; however, some authors believe that mecillinam has potential in veterinary medicine if administered parenterally [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

The Prevalence and Characterization of Fecal Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Pigs on Farms of Different Sizes in Latvia

2021

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The aim of this study was to determine the prevalence of fecal ESBL-producing Escherichia coli (E. coli) in pigs on large and small farms in Latvia, to characterize beta-lactamase genes and establish an antimicrobial resistance profile. Fecal samples (n = 615) were collected from 4-week, 5-week, 6-week, 8-week, 12-week and 20-week-old piglets, pigs and sows on four large farms (L1, L2, L3, L4) and three small farms (S1, S2, S3) in Latvia. ChromArt ESBL agar and combination disc tests were used for the screening and confirmation of ESBL-producing E. coli. The antimicrobial resistance was determined by the disc diffusion method and ESBL genes were determined by polymerase chain reaction (PCR). Subsequently, ESBL-producing E. coli was confirmed on three large farms, L1 (64.3%), L2 (29.9%), L3 (10.7%) and one small farm, S1 (47.5%); n = 144 (23.4%). The prevalence of ESBL-producing E. coli differed considerably between the large and small farm groups (26.9% vs. 12.7%). Of ESBL E. coli isolates, 96% were multidrug-resistant (MDR), demonstrating there were more extensive MDR phenotypes on large farms. The distribution of ESBL genes was blaTEM (94%), blaCTX-M (94%) and blaSHV (48%). On the small farm, blaSHV dominated, thus demonstrating a positive association with resistance to amoxicillin-clavulanic acid, ceftazidime and cefixime, while on the large farms, blaCTX-M with a positive association to cephalexin and several non-beta lactam antibiotics dominated. The results indicated the prevalence of a broad variety of ESBL-producing E. coli among the small and large farms, putting the larger farms at a higher risk. Individual monitoring of ESBL and their antimicrobial resistance could be an important step in revealing hazardous MDR ESBL-producing E. coli strains and reviewing the management of antibiotic use.

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“…Only DNA extracted from embryonated eggs or axenic culture was used for WGS, since the DNA quantity deriving directly from field samples was insufficient for sequencing. WGS was performed on Illumina MiSeq platform using standard protocols (Garcia-Graells et al, 2020). For sequences obtained from embryonated eggs, read quality and filtering was calculated by ShortRead 1.16.331 package (Morgan et al, 2009) (Bioconductor 3 ) and FastX 0.0.1333 (FastX-toolkit 4 ), while adapters were trimmed with cutadapt 1.2 (Martin, 2011).…”

Section: Wgs Sequencing and In Silico Genotypingmentioning

confidence: 99%

Phylogeography of Human and Animal Coxiella burnetii Strains: Genetic Fingerprinting of Q Fever in Belgium

Tomaiuolo

Boarbi

Fancello

et al. 2021

Front. Cell. Infect. Microbiol.

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Q fever is a zoonotic disease caused by the bacteria Coxiella burnetii. Domestic ruminants are the primary source for human infection, and the identification of likely contamination routes from the reservoir animals the critical point to implement control programs. This study shows that Q fever is detected in Belgium in abortion of cattle, goat and sheep at a different degree of apparent prevalence (1.93%, 9.19%, and 5.50%, respectively). In addition, and for the first time, it is detected in abortion of alpaca (Vicugna pacos), raising questions on the role of these animals as reservoirs. To determine the relationship between animal and human strains, Multiple Locus Variable-number Tandem Repeat Analysis (MLVA) (n=146), Single-Nucleotide Polymorphism (SNP) (n=92) and Whole Genome Sequencing (WGS) (n=4) methods were used to characterize samples/strains during 2009-2019. Three MLVA clusters (A, B, C) subdivided in 23 subclusters (A1-A12, B1-B8, C1-C3) and 3 SNP types (SNP1, SNP2, SNP6) were identified. The SNP2 type/MLVA cluster A was the most abundant and dispersed genotype over the entire territory, but it seemed not responsible for human cases, as it was only present in animal samples. The SNP1/MLVA B and SNP6/MLVA C clusters were mostly found in small ruminant and human samples, with the rare possibility of spillovers in cattle. SNP1/MLVA B cluster was present in all Belgian areas, while the SNP6/MLVA C cluster appeared more concentrated in the Western provinces. A broad analysis of European MLVA profiles confirmed the host-species distribution described for Belgian samples. In silico genotyping (WGS) further identified the spacer types and the genomic groups of C. burnetii Belgian strains: cattle and goat SNP2/MLVA A isolates belonged to ST61 and genomic group III, while the goat SNP1/MLVA B strain was classified as ST33 and genomic group II. In conclusion, Q fever is widespread in all Belgian domestic ruminants and in alpaca. We determined that the public health risk in Belgium is likely linked to specific genomic groups (SNP1/MLVA B and SNP6/MLVA C) mostly found in small ruminant strains. Considering the concordance between Belgian and European results, these considerations could be extended to other European countries.

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First detection of a plasmid located carbapenem resistant blaVIM-1 gene in E. coli isolated from meat products at retail in Belgium in 2015

Cited by 17 publications

References 33 publications

Identification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

Identification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

The Prevalence and Characterization of Fecal Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Pigs on Farms of Different Sizes in Latvia

Phylogeography of Human and Animal Coxiella burnetii Strains: Genetic Fingerprinting of Q Fever in Belgium

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