First detection and complete genome sequence of a new tobamovirus naturally infecting Hibiscus rosa-sinensis in Hawaii

Meta-Transcriptomic Analysis Uncovers the Presence of Four Novel Viruses and Multiple Known Virus Genera in a Single Hibiscus rosa-sinensis Plant in Colombia

“…High-throughput sequencing (HTS) is a powerful tool for virus detection and the discovery of many hibiscus viruses worldwide in the last few years. So far, at least 23 viruses have been reported as infecting ornamental hibiscus worldwide, including members of the genera Alfamovirus (alfalfa mosaic virus) [ 9 ], Betacarmovirus (hibiscus chlorotic ringspot virus) [ 10 ], Begomovirus (cotton leaf curl Multan virus [ 11 , 12 ], cotton leaf curl Burewala virus [ 13 ], hibiscus golden mosaic virus [ 14 ], and hibiscus yellow vein leaf curl virus) [ 15 ], Cilevirus (citrus leprosis virus C, CiLV-C2) [ 5 , 6 , 7 ], hibiscus-infecting cilevirus [ 16 ], and passion fruit green spot virus (PFGSV) [ 17 ], and an unclassified cilevirus (hibiscus yellow blotch virus) [ 18 ], Dichorhavirus (citrus chlorotic spot virus [ 19 ], and clerodendrum chlorotic spot virus) [ 20 ], Higrevirus (hibiscus green spot virus 2) [ 21 ], Nucleorhabdovirus (eggplant mottled dwarf virus) [ 22 ], Ilarvirus (tobacco streak virus) [ 23 ], Tobamovirus (hibiscus latent Fort Pierce virus, HLFPV, [ 24 ], hibiscus latent Singapore virus, HLSV [ 25 ], hibiscus latent Hawaii virus [ 26 ], and tomato mosaic virus) [ 27 ], Tymovirus (okra mosaic virus) [ 28 , 29 ], Tospovirus (impatiens necrotic spot virus) [ 30 ], and Soymovirus (HSV) [ 31 ]. Except for the genera Begomovirus and Soymovirus , the viral genomes of all of the above-mentioned genera consist of ribonucleic acid (RNA).…”