First description of Coxiella burnetii and Rickettsia spp. infection and molecular detection of piroplasma co-infecting horses in Xinjiang Uygur Autonomous Region, China

Li, Jixu; Li, Yongchang; Moumouni, Paul Franck Adjou; Lee, Seunghun; Galon, Eloiza May; Tumwebaze, Maria Agnes; Yang, Hongxia; Huercha,; Liu, Mingming; Guo, Huanping; Gao, Yang; Byamukama, Benedicto; Zhang, Wei; Fan, Xinli; Chahan, Bayin; Xu, Xuan

doi:10.1016/j.parint.2019.102028

Cited by 23 publications

(20 citation statements)

References 45 publications

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“…The 10 µL PCR reaction mixture was used in the present study, containing 3 µL yak DNA template of blood, 0.1 µL Taq polymerase (0.5 U; New England BioLab, Ipswich, MA, USA), 0.5 µL each of forward and reverse primer (100 µM), 1 µL 10×ThermoPol Reaction Buffer (New England BioLab), 0.2 µL deoxyribonucleotide triphosphate (200 µM; New England BioLab), and double-distilled water up to 10 µL. Positive animal DNA samples from a previous study [8] were used as positive controls, and double-distilled water was used as a negative control.…”

Section: Methodsmentioning

confidence: 99%

“…In China, eleven SFG Rickettsia species, Rickettsia sibirica, Rickettsia heilongjiangensis, Rickettsia aeschlimannii, Rickettsia conorii, Rickettsia felis, Rickettsia massiliae, Rickettsia monacensis, Rickettsia rickettsia, Candidatus Rickettsia jingxinensis, Rickettsia raoultii and Rickettsia slovaca, have been identified in tick vectors, Pathogens 2020, 9, 249 2 of 9 domestic animals and wildlife [5][6][7][8][9][10][11][12][13]. Several Rickettsia genotypes have been characterized as etiological factors of human rickettsiosis in the country, such as R. heilongjiangensis [14,15], R. sibirica subsp.…”

Section: Introductionmentioning

confidence: 99%

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Human Spotted Fever Group Rickettsia Infecting Yaks (Bos grunniens) in the Qinghai-Tibetan Plateau Area

Jian

Moumouni

et al. 2020

Pathogens

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The Qinghai-Tibetan Plateau Area (QTPA) is a plateau with the highest average altitude, located in Northwestern China. There is a risk for interspecies disease transmission, such as spotted fever rickettsioses. However, information on the molecular characteristics of the spotted fever group (SFG) Rickettsia spp. in the area is limited. This study performed screenings, and detected the DNA of human pathogen, SFG Rickettsia spp., with 11.3% (25/222) infection rates in yaks (Bos grunniens). BLASTn analysis revealed that the Rickettsia sequences obtained shared 94.3–100% identity with isolates of Rickettsia spp. from ticks in China. One Rickettsia sequence (MN536161) had 100% nucleotide identity to two R. raoultii isolates from Chinese Homo sapiens, and one isolate from Qinghai Dermacentor silvarum. Meanwhile, another Rickettsia sequence (MN536157) shared 99.1–99.5% identity to one isolate from Dermacentor spp. in China. Furthermore, the phylogenetic analysis of SFG Rickettsia spp. ompA gene revealed that these two sequences obtained from yaks in the present study grouped with the R. slovaca and R. raoultii clades with isolates identified from Dermacentor spp. and Homo sapiens. Our findings showed the first evidence of human pathogen DNA, SFG Rickettsia spp., from animals, in the QTPA.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human Spotted Fever Group Rickettsia Infecting Yaks (Bos grunniens) in the Qinghai-Tibetan Plateau Area

Jian

Moumouni

et al. 2020

Pathogens

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“…The first Chinese strain of C. burnetii (Qi Yi) was isolated from a confirmed Q fever patient in 1962 [ 50 ]. In the past few decades, C. burnetii DNA has been detected in blood samples of human (33.33%, 8/24), goats (25.00%, 4/16), horses (39.50%, 79/200; 22.22%, 4/18), cattle (20.51%, 40/195) from Xinjiang [ 51 , 52 ], goats from Beijing (4.55%, 2/44) [ 53 ], spleen samples of mice in Yunnan (85.19%, 46/54) [ 54 ], in ticks from Gansu, Ningxia, Shanxi, Xinjiang, Jilin, Liaoning, Heilongjiang [ 39 , 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Coxiella burnetii is widespread in ticks (Ixodidae) in the Xinjiang areas of China

Lin²,

et al. 2020

BMC Vet Res

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Background: The gram-negative Coxiella burnetii bacterium is the pathogen that causes Q fever. The bacterium is transmitted to animals via ticks, and manure, air, dead infected animals, etc. and can cause infection in domestic animals, wild animals, and humans. Xinjiang, the provincial-level administrative region with the largest land area in China, has many endemic tick species. The infection rate of C. burnetii in ticks in Xinjiang border areas has not been studied in detail. Results: For the current study, 1507 ticks were collected from livestock at 22 sampling sites in ten border regions of the Xinjiang Uygur Autonomous region from 2018 to 2019. C. burnetii was detected in 205/348 (58.91%) Dermacentor nuttalli; in 110/146 (75.34%) D. pavlovskyi; in 66/80 (82.50%) D. silvarum; in 15/32 (46.90%) D. niveus; in 28/132 (21.21%) Hyalomma rufipes; in 24/25 (96.00%) H. anatolicum; in 219/312 (70.19%) H. asiaticum; in 252/338 (74.56%) Rhipicephalus sanguineus; and in 54/92 (58.70%) Haemaphysalis punctata. Among these samples, C. burnetii was detected in D. pavlovskyi for the first time. The infection rate of Rhipicephalus was 74.56% (252/338), which was the highest among the four tick genera sampled, whereas the infection rate of H. anatolicum was 96% (24/25), which was the highest among the nine tick species sampled. A sequence analysis indicated that 63 16S rRNA sequences could be found in four newly established genotypes: MT498683.1 (n = 18), MT498684.1 (n = 33), MT498685.1 (n = 6), and MT498686.1 (n = 6). Conclusions: This study indicates that MT498684.1 might represent the main C. burnetii genotype in the ticks in Xinjiang because it was detected in eight of the tick species studied. The high infection rate of C. burnetii detected in the ticks found in domestic animals may indicate a high likelihood of Q fever infection in both domestic animals and humans.

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“…this result revealed that age may be a risk factor for C. burnetii in horses. The difference may be explained as a consequence of a higher probability of contact [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Coxiella burnetii in horse sera in Iran

Khademi

Ownagh

Ataei

et al. 2020

Comparative Immunology, Microbiology and Infectious Diseases

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Highlights In this manuscript, we detect the Coxiella burnetii DNA by Molecular Method in horse sera for the first time in Iran. Coxiella burnetii is a high pathogenic organism that can cause Q fever with 1–10 Numbers of bacteria. Important as zoonotic disease, its circulation dynamics in and through horses are still unclear.

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First description of Coxiella burnetii and Rickettsia spp. infection and molecular detection of piroplasma co-infecting horses in Xinjiang Uygur Autonomous Region, China

Cited by 23 publications

References 45 publications

Human Spotted Fever Group Rickettsia Infecting Yaks (Bos grunniens) in the Qinghai-Tibetan Plateau Area

Human Spotted Fever Group Rickettsia Infecting Yaks (Bos grunniens) in the Qinghai-Tibetan Plateau Area

Coxiella burnetii is widespread in ticks (Ixodidae) in the Xinjiang areas of China

Molecular detection of Coxiella burnetii in horse sera in Iran

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