First Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Sulpiride and Mebeverine Hydrochloride in Their Combined Tablets and Application to Real Human Plasma

Walash, M. I.; El-Din, M. Sharaf; El‐Enany, N.; Eid, Manal; Shalan, Shereen

doi:10.1007/s10895-010-0679-0

Cited by 25 publications

(17 citation statements)

References 37 publications

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“…MEB and SUL were determined in their binary mixture via derivative spectroscopy [3,4], first derivative synchronous fluorescence spectroscopy [5]. TLC- densitometry [4], HPLC [4-6] and chemometric techniques [7].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Walash

El-Din

El‐Enany

et al. 2012

Chemistry Central Journal

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A new simple, rapid and sensitive reversed-phase liquid chromatographic method was developed and validated for the simultaneous determination of sulpiride (SUL) and mebeverine Hydrochloride (MEB) in the presence of their impurities and degradation products. The separation of these compounds was achieved within 6 min on a 250 mm, 4.6 mm i.d., 5 m particle size Waters®-C18 column using isocractic mobile phase containing a mixture of acetonitrile and 0.01 M dihydrogenphosphate buffer (45:55) at pH = 4.0. The analysis was performed at a flow rate of 1.0 mL/min with fluorescence-detection at excitation 300 nm and emission at 365 nm. The concentration-response relationship was linear over a concentration range of 10- 100 ng/mL for both MEB and SUL with a limit of detection 0.73 ng/mL and 0.85 ng/mL for MEB and SUL respectively. The proposed method was successfully applied for the analysis of both MEB and SUL in bulk with average recoveries of 100.22 ± 0.757% and 99.96 ± 0.625% respectively, and in commercial tablets with average recoveries of 100.04 ± 0.93% and 100.03 ± 0.376% for MEB and SUL respectively. The proposed method was successfully applied to the determination of MEB metabolite (veratic acid) in real plasma simultaneously with SUL. The mean% recoveries (n = 3) for both MEB metabolite (veratic acid) and SUL were 100.36 ± 2.92 and 99.06 ± 2.11 for spiked human plasma respectively. For real human plasma, the mean% recoveries (n = 3) were and respectively.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Walash

El-Din

El‐Enany

et al. 2012

Chemistry Central Journal

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“…According to the traditional organic solvents compared by EHS and LCA, EHS‐preferred solvents were methanol, ethanol, and methyl acetate. Life‐cycle‐preferred solvents were hexane, heptane, and diethyl ether .…”

Section: Resultsmentioning

confidence: 99%

Validated green high‐performance liquid chromatographic methods for the determination of coformulated pharmaceuticals: A comparison with reported conventional methods

Elzanfaly

Hegazy

Saad

et al. 2015

J of Separation Science

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The introduction of sustainable development concepts to analytical laboratories has recently gained interest, however, most conventional high-performance liquid chromatography methods do not consider either the effect of the used chemicals or the amount of produced waste on the environment. The aim of this work was to prove that conventional methods can be replaced by greener ones with the same analytical parameters. The suggested methods were designed so that they neither use nor produce harmful chemicals and produce minimum waste to be used in routine analysis without harming the environment. This was achieved by using green mobile phases and short run times. Four mixtures were chosen as models for this study; clidinium bromide/chlordiazepoxide hydrochloride, phenobarbitone/pipenzolate bromide, mebeverine hydrochloride/sulpiride, and chlorphenoxamine hydrochloride/caffeine/8-chlorotheophylline either in their bulk powder or in their dosage forms. The methods were validated with respect to linearity, precision, accuracy, system suitability, and robustness. The developed methods were compared to the reported conventional high-performance liquid chromatography methods regarding their greenness profile. The suggested methods were found to be greener and more time- and solvent-saving than the reported ones; hence they can be used for routine analysis of the studied mixtures without harming the environment.

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“…These include gas chromatography (GC) (8,9), spectrofluorimetric techniques (10,11), high-performance liquid chromatographic (HPLC) methods with UV detection (12)(13)(14), HPLC with fluorescence detection (15,16), capillary electrophoresis (17,18) and adsorptive stripping voltammetry (19). These include gas chromatography (GC) (8,9), spectrofluorimetric techniques (10,11), high-performance liquid chromatographic (HPLC) methods with UV detection (12)(13)(14), HPLC with fluorescence detection (15,16), capillary electrophoresis (17,18) and adsorptive stripping voltammetry (19).…”

Section: Introductionmentioning

confidence: 99%

Determination of sulpiride in pharmaceutical preparations and biological fluids using a Cr (III) enhanced chemiluminescence method

et al. 2012

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A highly sensitive and simple method for identifying sulpiride in pharmaceutical formulations and biological fluids is presented. The method is based on increased chemiluminescence (CL) intensity of a luminol-H2O2 system in response to the addition of Cr (III) under alkaline conditions. The CL intensity of the luminol-H2O2-Cr (III) system was greatly enhanced by the addition of sulpiride and the CL intensity was proportional to the concentration of sulpiride in a sample solution. Various parameters affecting the CL intensity were systematically investigated and optimized for determination of the sulpiride in a sample. Under the optimum conditions, the CL intensity was proportional to the concentration of sulpiride in the range of 0.068-4.0 µg/mL, with a good correlation coefficient of 0.997. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 8.50 × 10(-6) µg/mL and 2.83 × 10(-5) µg/mL, respectively. The method presented here produced good reproducibility with a relative standard deviation (RSD) of 2.70% (n = 7). The effects of common excipients and metal ions were studied for their interference effect. The method was validated statistically through recovery studies and successfully applied for the determination of sulpiride in pure form, pharmaceutical preparations and spiked human plasma samples. The percentage recoveries were found to range from 99.10 to 100.05% for pure form, 98.12 to 100.18% for pharmaceutical preparations and 97.9 to 101.4% for spiked human plasma.

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First Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Sulpiride and Mebeverine Hydrochloride in Their Combined Tablets and Application to Real Human Plasma

Cited by 25 publications

References 37 publications

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Simultaneous determination of sulpiride and mebeverine by HPLC method using fluorescence detection: application to real human plasma

Validated green high‐performance liquid chromatographic methods for the determination of coformulated pharmaceuticals: A comparison with reported conventional methods

Determination of sulpiride in pharmaceutical preparations and biological fluids using a Cr (III) enhanced chemiluminescence method

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