First confirmed report on Fusarium sporotrichioides on Pinus ponderosa var. jeffreyi in Slovakia

Abstract

Ivanová H., Hrehová Ľ., Pristaš P. (2016): First confirmed report on Fusarium sporotrichioides on Pinus ponderosa var. jeffreyi in Slovakia. Plant Protect. Sci., 52: 250-253.During 2014-2015, samples from ten 20-25 years old pine (Pinus ponderosa var. jeffreyi) trees showing wilt symptoms were collected from the Arborétum Mlyňany park. A disease was observed on 20% of the trees. The first symptoms are wilting, stunting, chlorosis, and discolouration of needles, which turned yellow on affected twigs, then red and finally they fell off. Isolations of the pathogen were done from the discoloured tissues of needles (twenty samples from each tree) on Potato Dextrose Agar. Colonies of the fungus (3-4 Petri dishes from each tree) were initially aerial, white or slightly violet, but with age they became red and red pigments were produced in agar. The observed micromorphological characteristics of the fungus, such as presence of simple and proliferating conidiophores with polyphialides, microconidia, macroconidial shape, and chlamydospore presence matched the description of Fusarium sporotrichioides. The identity of the fungus was confirmed by phylogenetic analysis of internal transcribed spacer (ITS) sequences. Sequence comparisons placed the fungus to the species F. sporotrichioides with similarity of 99.6% at the ITS sequence level.

Searching thePinus taedafoliar mycobiome for emerging pathogens among brown spot needle blight and needlecast outbreaks in the Southeast United States

“…Experiments must be conducted to determine whether N. microsporum may be a pathogen on P. taeda or if co-infection with L. acicola may yield synergistic or antagonistic interactions. Fusarium sporotrichioides, which we identified as a moderate predictor of needlecast symptoms is a well-known mycotoxigenic fungus and cereal crop pathogen and was recently reported in association with wilt and defoliation on P. ponderosa in Slovakia (Ivanová et al 2016). As Fusarium spp.…”

“…The identities of potential pathogens detected in pine needle samples are reported in Table 4. While the taxonomic classifier was trained using the curated UNITE database and produced high-confidence assignments, the identification of fungal taxa using a single locus can often lack resolution and the reported identities should all be considered preliminary (Ivanová et al 2016). Several known needle pathogens were identified among sequences from both symptomatic and asymptomatic needles from sampled trees.…”

“…Lecanosticta acicola and Neocatenulostroma microsporum (Taylor & Crous) Quaedvl. & Crous were identified as strong predictors of the observed BSNB symptoms, while indicators of needlecast included multiple ESVs assigned to the P. taeda needle endophyte and weak pathogen Lophodermium conigenum, the cereal crop and pine pathogen Fusarium sporotrichioides (Ivanová et al 2016), and unidentified members of the genus Coleosporium and the family Teratosphaeriaceae (Figure 3).…”

“…Fusarium sporotrichioides, which we identified as a moderate predictor of needlecast symptoms is a well-known mycotoxigenic fungus and cereal crop pathogen and was recently reported in association with wilt and defoliation on P. ponderosa in Slovakia(Ivanová et al 2016) Aime et al 2017)…”

The culturable leaf mycobiome of Viscum album subsp. austriacum

“…The disease symptoms on mistletoe leaves may also be caused by F. sporotrichioides, which was isolated from leaves with symptom A. This fungus is widespread across tropical and temperate regions (Domsch et al, 2007) and is commonly associated with seedling diseases in forest nurseries (James & Dumroese, 2007) and cankers, wilt symptoms and needle dieback on mature trees (Anderson, 1986;Ivanová et al, 2016). Another interesting Fusarium species obtained from diseased mistletoe leaves was Fusarium juglandicola.…”