First confirmed report of bacterial wilt of tomato in Georgia caused by <i>Ralstonia solanacearum</i>

Mepharishvili, Galina; Sikharulidze, Z.; Thwaites, Richard; Tsetskhladze, T.; Dumbadze, R.; Gabaidze, Mziuri; Muradashvili, M.

doi:10.5197/j.2044-0588.2012.025.016

Cited by 5 publications

(5 citation statements)

References 6 publications

(4 reference statements)

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“…Bacterial strains: 40 strains of R. solanacearum used in this study were isolated from samples collected in different regions of Georgia, mainly in the West and South Georgia (Muradashvili et al, 2012;2014).…”

Section: Materials and Methods:-mentioning

confidence: 99%

The Isolation and Biological Characterization of Bacteriophages Lytic to Georgian Strains of Ralstonia Solanacearum Race 3 Biovar 2.

Lashkhi¹,

Kokashvili²,

Eliashvili³

et al. 2018

IJAR

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Antibiotic sensitivity revealed that all isolates were fully susceptible to enerofloxacin, ciprofloxacin, while all isolates were fully resistant to penicillin. E. coli serogrouped isolates were subjected to PCR for detection of Stx1 and Stx2 genes. 3 out of 7 serogrouped isolates (42.85%) were carried Stx2 gene (O55 and O27 from contact human and O86 from mastitic milk) while Stx1 gene was not detected . phylogenetic analysis for the sequence data of the Sxt2 gene of E. coli serogroupes revealed that Sxt2 gene isolated from mastitic milk of cattle is closely identical (100% identity) to Sxt2 gene isolated from contact human. In Conclusion, isolation of STEC from cattle might have potential pathogenicity for human. So that contact human should use sound hygienic measures during milking and management of these animals to avoid zoonotic infection. Ralstonia solanacearum is a soilborne bacterial pathogen, distributed in tropical, subtropical and some temperate regions, causing serious diseases of strategic crops, such as potato, tomato, banana etc. Control of plant infections caused by R.solanacearum is a challenging issue. Phage preparations can be considered as effective tool for prevention of infection spread in the environment and in the seed plant material. Since first report on detection of R. solanacearum in Georgia up to 60 strains have been collected, majority attributed to race 2 biovar 3. The presented work aimed at isolation of bacteriophages specific to Georgian strains of R. solanacearum and study their biological properties in order to select phages for strain subtyping and for infection control. During 2015-2017 enrichment of environmental samples with 40 selected isolates of R. solanacearum and subsequent processing of primary mixtures resulted in obtaining of 25 individual Rs phages. Initial grouping of phages was done based on negative colony morphology, lytic spectrum and virion ultrastructure. Transmission electron micrioscopy (TEM) revealed the prevalence of Myoviridae type morphology among Rs phages, although single phages were attributed to Podoviridae and Siphoviridae families. The Rs phages showed diverse host range. The phage -and antibiotic susceptibility profiles of R. solanacearum strains were compared and showed no correlation between these two characteristics.Ten

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Section: Materials and Methods:-mentioning

confidence: 99%

The Isolation and Biological Characterization of Bacteriophages Lytic to Georgian Strains of Ralstonia Solanacearum Race 3 Biovar 2.

Lashkhi¹,

Kokashvili²,

Eliashvili³

et al. 2018

IJAR

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“…Our investigations have therefore confirmed that R. solanacearum is present and causing wilt disease of tomato in Georgia. This was the first report of bacterial wilt caused by R. solanacearum in Georgia [3]. Since 2010, bacterial wilt in tomato presumed to be caused by R. solanacearum has been an important disease in Georgia as this is a valuable crop for farmers in many regions across Georgia.…”

Section: Plant Quarantine Phytopathogenic Microorganismsmentioning

confidence: 99%

Study of quarantine plant diseases in Georgia

Meparishvili¹,

Gorgiladze²,

Sikharulidze³

et al. 2018

ZKR

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Our Organization has been conducting plant diseases monitoring and diagnostics of their causal agents. We found the following quarantine pathogens: Dickeya sp., Ralstonia solanacearum, Erwinia amylovora, Pseudomonas syringae pv. actinidiae, Synchytrium endobioticum. To fix diseases on time and study their causing pathogens, is fundamental for the country safety and makes it possible not to infect a huge territory.

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“…In tomato, bacterial wilt was identified for the first time in 2011 in Chkorotsku and Kutaisi regions (west Georgia). Where it caused up to 100% plant loss in greenhouse-and field-grown crops [3]. Since then, several cases of this disease have been documented on potato in home gardens [4].…”

Section: Tomato Ralstonia Solanacearum Early Blight Late Blight Rmentioning

confidence: 99%

Screening of tomato varieties for resistance to major fungal diseases and bacterial wilt

Tsetskladze¹,

Sikharulidze²,

Muradashvili³

et al. 2018

ZKR

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The resistance of local and introduced tomato varieties to early blight (Alternaria solani Sorauer) and late blight (Phytopthora infestans (Mont.) de Bary) were evaluated in the field and to bacterial wilt (Ralstonia solanacearum) were tested under artificial infection in greenhouse conditions. Three varieties (De-brao krasnii, De-brao Rozovii and Sultan F1) showed the moderate resistance to early blight, while the only two varieties (De-brao krasnii and De-brao Rozovii) were moderate resistant to late blight. The remaining samples showed susceptibility to disease. The tested varieties showed a different degree of resistance to the bacterial wilt. Four varieties: Rozovii Gigant, Krachodarskii Krasnii,Tarasenko and Lagidnii appeared to be resistant; Two varieties: Fakel and Polbig showed the moderate resistance and the rest ten varieties were susceptible to the disease.

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First confirmed report of bacterial wilt of tomato in Georgia caused by Ralstonia solanacearum

Cited by 5 publications

References 6 publications

The Isolation and Biological Characterization of Bacteriophages Lytic to Georgian Strains of Ralstonia Solanacearum Race 3 Biovar 2.

The Isolation and Biological Characterization of Bacteriophages Lytic to Georgian Strains of Ralstonia Solanacearum Race 3 Biovar 2.

Study of quarantine plant diseases in Georgia

Screening of tomato varieties for resistance to major fungal diseases and bacterial wilt

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