First confirmed case of Alaria alata mesocercaria in Eurasian lynx (Lynx lynx) hunted in Latvia

Ozoliņa, Zanda; Bagrade, Guna; Deksne, Gunita

doi:10.1007/s00436-019-06556-8

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Thominx aerophilus) and Pearsonema feliscati (syn. Capilaria felis-cati)) and a single tapeworm (Taenia pisiformis) and trematode (Alaria alata) (Bagrade et al 2003;Deksne et al 2016;Ozoliņa et al 2019). A broader spectrum of helminths has been detected in grey wolves from Latvia.…”

Section: Resultsmentioning

confidence: 99%

The first records of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae), a causative agent of human sparganosis, in Latvian wildlife

et al. 2020

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Diphyllobothriid tapeworms of the genus Spirometra are causative agents of sparganosis, food-borne zoonotic parasitic disease. They have been recorded in broad spectrum of hosts, including humans, in all continents except Antarctica. Spirometra tapeworms have been intensively studied in several Asian countries; however, they have been rather neglected in Europe. The aim of this study was to provide a pilot screening of Spirometra spp. in Latvia, where data on sparganosis are not available. Tapeworms morphologically identified as diphyllobothriid species were isolated from grey wolves Canis lupus and Eurasian lynxes Lynx lynx from Latvia during the hunting periods 2013–2019. The parasites were subjected to molecular genotyping using sequences of the partial large (LSU rDNA; 615 bp) and small (SSU rDNA; 720 bp) subunits of the nuclear ribosomal RNA gene and complete (1566 bp) cytochrome c oxidase subunit I gene of the mitochondrial DNA (cox1 mtDNA). Analyses of both ribosomal subunits of 13 tapeworms revealed no intraspecific variation within the respective rDNA subunits. On the other hand, sequence analysis of mitochondrial cox1 revealed intraspecific polymorphism displayed by 12 cox1 haplotypes. Comparison of the current data with sequences of the corresponding DNA regions deposited in the GenBank revealed 99.3–99.5% (LSU rDNA), 99.2% (SSU rDNA) and 99.6–100% (cox1 mtDNA) identity of studied tapeworms with Spirometra erinaceieuropaei, which provided the first confirmation of this diphyllobothriid tapeworm in Latvia. Since S. erinaceieuropaei is probably prevalent in Latvian wildlife and may also occur in other potential host species, further studies are needed in order to acquire complex data on its geographic distribution and transmission in the natural environment of Latvia, as well as on the spectrum of its intermediate, paratenic, and definitive hosts.

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Section: Resultsmentioning

confidence: 99%

The first records of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae), a causative agent of human sparganosis, in Latvian wildlife

et al. 2020

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“…Therefore, in paratenic hosts L3 can be found in cysts as small white spots in different body sites, including striated muscle ( Sen and Anantaraman, 1971 ). The phenomenon of one host species serving both as main- and paratenic host has been described for other parasites (e.g., for Alaria alata in European lynx, Lynx lynx ) ( Zanda Ozoliņa et al, 2020 ) and might be due to a strategy to complete the biological life cycle of the parasites through the trophic chain of predation ( Mendoza Roldan and Otranto, 2023 ). The method employed for the detection of Trichinella larvae is not the most suitable method to detect Spirocerca sp.…”

Section: Discussionmentioning

confidence: 99%

Detection of Spirocerca lupi and an unknown Trichinella-like nematode in raccoon (Procyon lotor)

Langner,

Otranto,

Bezerra-Santos

et al. 2024

International Journal for Parasitology: Parasites and Wildlife

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“…A total of 61 AM samples from different host individuals were collected during a prevalence study from 2017 to 2020 in Brandenburg, Germany [ 11 ], in collaboration with the Food Inspection and Veterinary Department, Administrative District Görlitz (State of Saxony, Germany), the LADR GmbH Medical Care Center North in Flintbek (State of Schleswig-Holstein, Germany), the local veterinary office in Brodnica (Brodnica, Poland) and the Institute of Food Safety, Animal Health and Environment (BIOR) in Riga, Latvia [ 10 , 33 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

et al. 2021

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Alaria (A.) alata mesocercariae (AM) have increasingly appeared as incidental findings during the mandatory inspection of wild boars for Trichinella in many European countries. An Alaria spp.-specific PCR is available for the identification of AM; however, it is time- and cost-intensive. Therefore, we propose a rapid and cost-efficient MALDI-TOF assay for the identification of AM in wild boar meat that can be applied in routine diagnostics. In this study, a fast and methodologically simple protocol for the protein extraction of AM from different host species in different countries was established, and an AM-specific reference spectra database was created as part of the ongoing development of an existing Trichinella spp. database. A formic acid protein extraction was performed after pooling 10 AM from the same host individual. In total, 61 main spectra profiles (MSPs) from different host individuals were stored in an AM-specific MSP library. The cluster analysis of these 61 MSPs indicated a possible variation within the A. alata species with a tentative association with the geographical origin of the host, but not the host species. This MALDI-TOF assay allows for a fast verification of the AM isolates, which is the next step in the development of a universal database for the identification of several parasites isolated from meat.

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First confirmed case of Alaria alata mesocercaria in Eurasian lynx (Lynx lynx) hunted in Latvia

Cited by 6 publications

References 23 publications

The first records of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae), a causative agent of human sparganosis, in Latvian wildlife

The first records of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae), a causative agent of human sparganosis, in Latvian wildlife

Detection of Spirocerca lupi and an unknown Trichinella-like nematode in raccoon (Procyon lotor)

Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

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