First Complete Genome Sequence of Tenacibaculum dicentrarchi, an Emerging Bacterial Pathogen of Salmonids

Grothusen, Horst; Castillo, Alejandro; Henríquez, Patricio; Navas, Esteban; Bohle, H.; Araya, Claudia; Bustamante, Fernando; Bustos, Patricio; Mancilla, Marcos

doi:10.1128/genomea.01756-15

Cited by 17 publications

(17 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, T. maritimum does not possess a proteorhodopsin-encoding gene as identified in close relatives such as Polaribacter , Dokdonia , or Psychroflexus species, suggesting the inability of this bacterium to use light to generate proton motive force. Comparison with the available genomes of the three other fish-pathogenic Tenacibaculum species Tenacibaculum dicentrarchi , Tenacibaculum ovolyticum , and T. soleae ( Grothusen et al, 2016 ; Lujan et al, 2016 ; Teramoto et al, 2016 ), has revealed striking differences in virulence strategies as most, if not all, the aforementioned predicted toxins ( Table 1 ) are absent from the genomes of the latter species. These elements point to very different paths in the evolution of virulence as suggested using a subset of core-genome genes ( Habib et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

et al. 2017

View full text Add to dashboard Cite

Tenacibaculum maritimum is a devastating bacterial pathogen of wild and farmed marine fish with a broad host range and a worldwide distribution. We report here the complete genome sequence of the T. maritimum type strain NCIMB 2154T. The genome consists of a 3,435,971-base pair circular chromosome with 2,866 predicted protein-coding genes. Genes encoding the biosynthesis of exopolysaccharides, the type IX secretion system, iron uptake systems, adhesins, hemolysins, proteases, and glycoside hydrolases were identified. They are likely involved in the virulence process including immune escape, invasion, colonization, destruction of host tissues, and nutrient scavenging. Among the predicted virulence factors, type IX secretion-mediated and cell-surface exposed proteins were identified including an atypical sialidase, a sphingomyelinase and a chondroitin AC lyase which activities were demonstrated in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Concatenation of the gene sequences was done in a head-to-tail manner according to their physical order in the sequenced genome of the Tenacibaculum strain (Accession No. AY7486TD) (Grothusen et al, 2016). The evolutionary distance was computed using the maximum composite likelihood method (Tamura, Nei, & Kumar, 2004), and the evolutionary analyses were conducted in MEGA7 (Kumar, Stecher, & Tamura, 2016).…”

Section: Multilocus Sequence Analysis (Mlsa)mentioning

confidence: 99%

An outbreak of acute disease and mortality in Atlantic salmon (Salmo salar) post‐smolts in Norway caused by Tenacibaculum dicentrarchi

Klakegg

Abayneh

Fauske

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

An outbreak of disease characterized by skin ulcers, fin rot and mortality was observed a few days after the transfer of Atlantic salmon (Salmo salar) from a freshwater smolt production facility to a land‐based seawater post‐smolt site. Dead and moribund fish had severe skin and muscle ulcers, often 2–6 cm wide, particularly caudal to the pectoral fins. Microscopic examination of smears from ulcers and head kidney identified long, slender Gram‐negative rods. Histopathological analysis revealed abundance of long, slender Tenacibaculum‐like bacteria in ulcers and affected fins. Genetic characterization using multilocus sequence analysis (MLSA) of seven housekeeping genes, including atpA, dnaK, glyA, gyrB, infB, rlmN and tgt, revealed that the isolates obtained during the outbreak were all clustered with the Tenacibaculum dicentrarchi‐type strain (USC39/09T) from Spain. Two bath challenge experiments with Atlantic salmon and an isolate of T. dicentrarchi from the outbreak were performed. No disease or mortality was observed in the first trial. In the second trial with a higher challenge dose of T. dicentrarchi and longer challenge time, we got 100% mortality within 48 hr. This is the first reported outbreak of disease caused by T. dicentrarchi in Norwegian farmed Atlantic salmon.

show abstract

“…A total of 3,969 coding DNA sequences were annotated by RASTtk ( http://rast.nmpdr.org ). One copy of the 16S rRNA gene was present, showing 95.7 to 96.6% sequence identity to the genes (9 copies) in strain AY7486TD ( 8 ).…”

Section: Genome Announcementmentioning

confidence: 98%

“…In this genus, T. maritimum ( 1 ), T. ovolyticum ( 2 , 3 ), T. discolor , and T. gallaicum ( 4 , 5 ) are fish pathogens. T. soleae ( 6 ) and T. dicentrarchi ( 7 , 8 ) from diseased fish could also be pathogens. So far, the genome sequence has been published only for T. dicentrarchi AY7486TD, isolated from a diseased salmon ( 8 ).…”

Section: Genome Announcementmentioning

confidence: 99%

See 1 more Smart Citation

Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater

et al. 2016

View full text Add to dashboard Cite

Some bacterial species of the genus Tenacibaculum, including Tenacibaculum ovolyticum, have been known as fish pathogens in the sea. So far, the only published genome sequence for this genus is for Tenacibaculum dicentrarchi, which could also be a fish pathogen. Strain da5A-8, showing 100% identity to the 16S rRNA gene sequence of T. ovolyticum DSM 18103T, was isolated from seawater at a depth of 344 m in Kochi, Japan, and grew optimally at 10 to 20°C. The genome sequence of strain da5A-8 revealed the possible virulence genes commonly observed in the genus Tenacibaculum.

show abstract

First Complete Genome Sequence of Tenacibaculum dicentrarchi, an Emerging Bacterial Pathogen of Salmonids

Cited by 17 publications

References 7 publications

The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

An outbreak of acute disease and mortality in Atlantic salmon (Salmo salar) post‐smolts in Norway caused by Tenacibaculum dicentrarchi

Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater

Contact Info

Product

Resources

About