First Complete Genome Sequence of Currently Circulating Infectious Bronchitis Virus Strain DMV/1639 of the GI-17 Lineage

Goraichuk, Iryna V.; Kulkarni, Arun B.; Williams-Coplin, Dawn; Suarez, David L.; Afonso, Claudio L.

doi:10.1128/mra.00840-19

Cited by 14 publications

(14 citation statements)

References 16 publications

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“…Other molecular techniques, such as loop-mediated isothermal amplification (LAMP) and padlock probes (PLPs) combined with rolling circle amplification (RCA), have been validated for IBV detection [ 78 , 79 ]. Additionally, NGS techniques have recently been used to study IBV [ 80 , 81 ]. This approach, despite being currently too expensive and laborious for routine use, has proven useful for research purposes, particularly because it allows to precisely study the presence of subpopulations within a single sample and contextualize IBV within the respiratory disease complex.…”

Section: Approaches To Ibv Diagnosismentioning

confidence: 99%

Infectious Bronchitis Virus Evolution, Diagnosis and Control

Legnardi

Tucciarone

Franzo

et al. 2020

Veterinary Sciences

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RNA viruses are characterized by high mutation and recombination rates, which allow a rapid adaptation to new environments. Most of the emerging diseases and host jumps are therefore sustained by these viruses. Rapid evolution may also hinder the understanding of molecular epidemiology, affect the sensitivity of diagnostic assays, limit the vaccine efficacy and favor episodes of immune escape, thus significantly complicating the control of even well-known pathogens. The history of infectious bronchitis virus (IBV) fits well with the above-mentioned scenario. Despite being known since the 1930s, it still represents one of the main causes of disease and economic losses for the poultry industry. A plethora of strategies have been developed and applied over time, with variable success, to limit its impact. However, they have rarely been evaluated objectively and on an adequate scale. Therefore, the actual advantages and disadvantages of IBV detection and control strategies, as well as their implementation, still largely depend on individual sensibility. The present manuscript aims to review the main features of IBV biology and evolution, focusing on their relevance and potential applications in terms of diagnosis and control.

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Section: Approaches To Ibv Diagnosismentioning

confidence: 99%

Infectious Bronchitis Virus Evolution, Diagnosis and Control

Legnardi

Tucciarone

Franzo

et al. 2020

Veterinary Sciences

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“…Next-generation paired-end (2 × 150-bp) sequencing was performed on an Illumina MiSeq instrument using the 300-cycle MiSeq reagent kit version 2. The sequence data were assembled using a de novo approach and utilizing MIRA3 version 0.0.1 ( 19 ) within a customized workflow on the Galaxy platform ( 20 ), as described previously ( 21 , 22 ). The MiSeq run generated from 1,303,453 to 2,446,408 total paired-end reads per sample ( Table 1 ).…”

Section: Announcementmentioning

confidence: 99%

“…customized workflow on the Galaxy platform (20), as described previously (21,22). The MiSeq run generated from 1,303,453 to 2,446,408 total paired-end reads per sample ( Table 1).…”

mentioning

confidence: 99%

Complete Coding Sequences of Three Chicken Parvovirus Isolates from the United States

Goraichuk

Davis

Afonso

et al. 2020

Microbiol Resour Announc

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“…Next-generation paired-end sequencing (2 ϫ 250 bp) was performed on an Illumina MiSeq instrument using the 500-cycle MiSeq reagent kit v2 (Illumina, USA). Sequence data were assembled using a de novo approach and utilizing MIRA3 version 0.0.1 (10) within a customized workflow on the Galaxy platform (11), as described previously (12,13). The final outputs of the analysis workflow included a set of summary statistics on the run and the assembly.…”

mentioning

confidence: 99%