First Complete Genome Sequence of a Simian Foamy Virus Infecting the Neotropical Primate Brachyteles arachnoides

Muniz, Cláudia P.; Cavalcante, Liliane T. F.; Dudley, Dawn M.; Pissinatti, Alcides; O’Connor, David H.; Santos, André F.; Soares, Marcelo A.

doi:10.1128/mra.00839-18

Cited by 6 publications

(13 citation statements)

References 24 publications

(22 reference statements)

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“…To amplify larger DNA fragments, we developed new PCR primers only using sequences of representative SFV genomes infecting primates of Cebidae family to increase specificity. However, the new PCR amplified only 24% of samples diagnosed as SFV-positive, suggesting that there may be more variants circulating in the population, requiring more sensitive techniques, such as shotgun next-generation sequencing, to amplify the complete genomes of these viruses [6].…”

Section: Discussionmentioning

confidence: 99%

“…In NP, the prevalence is generally higher in animals in captivity (45–51%) compared to animals in the wild (14–30%) [2,3]. Although SFV has been described in at least 23 species of NP [3], there are only five complete genomes sequenced [6,7,8,9], a small number considering the high diversity of NP, distributed in at least 176 species, 17–21 genera and three to five families according to distinct classification systems [10,11]. Although the first NP SFV has been identified over four decades ago in cell cultures of spider monkey ( Ateles sp .)…”

Section: Introductionmentioning

confidence: 99%

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Eco-Epidemiological Profile and Molecular Characterization of Simian Foamy Virus in a Recently-Captured Invasive Population of Leontopithecus chrysomelas (Golden-Headed Lion Tamarin) in Rio de Janeiro, Brazil

Miranda

Muniz

Moreira³

et al. 2019

Viruses

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Simian foamy viruses (SFV) infect a wide range of Old World and Neotropical primates (NP). Unlike Old World primates, little is known about the diversity and prevalence of SFV in NP, mainly from a free-living population. Phylogenetic analyses have shown that SFV coevolved with their hosts. However, viral strains infecting Leontopithecus chrysomelas did not behave as expected for this hypothesis. The purpose of this study was to determine the eco-epidemiological profile and molecular characterization of SFV in a recently captured invasive population of L. chrysomelas located in Niteroi/RJ using buccal swab as an alternative collection method. A prevalence of 34.8% (32/92) and a mean viral load of 4.7 log copies of SFV/106 cells were observed. With respect to time since capture, SFV prevalence was significantly higher in the group of animals sampled over 6 months after capture (55.2%) than in those more recently captured (25.4%) (p = 0.005). Infected solitary animals can contribute to SFV transmission between different groups in the population. SFV strains formed two distinct clades within the SFV infecting the Cebidae family. This is the first study to use buccal swabs as a tool to study SFV diversity and prevalence in a recently free-living NP population upon recent capture.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Eco-Epidemiological Profile and Molecular Characterization of Simian Foamy Virus in a Recently-Captured Invasive Population of Leontopithecus chrysomelas (Golden-Headed Lion Tamarin) in Rio de Janeiro, Brazil

Miranda

Muniz

Moreira³

et al. 2019

Viruses

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“…Phylogenetic analysis of the three complete SFV genomes from NPs with those from OWPs and a prosimian showed SFVs clustering into three major clades, reflecting the evolutionary split between NP (parvorder Platyrhini), OWP (parvorder Catarrhini), and prosimian (Strepsirrhini suborder) hosts [20]. More recently, complete SFV genomes were obtained from Sapajus xanthosternos , the yellow-breasted capuchin (SFVsxa), and Brachyteles arachnoides , the wooly spider monkey (SFVbar) [21,22]. Additionally, partial SFV pol and/or LTR/ gag sequences (around 500-bp) were obtained from SFV strains infecting 20 different NP species from 10 genera, encompassing all three NP families (Table 1) [23,24,25].…”

Section: Diversity and Origin Of Sfvs In The Americasmentioning

confidence: 99%

“…The exception is the bovine FV pol gene, which is 3660-bp in length [21]. The size of the gag gene coding for the group specific antigen is conserved within both NP (range of 1817–2071-bp) and OWP SFVs (range of 1872–1974-bp) [21,22,33]. The exception is SFVssc, which has the shortest gag gene (1716-bp) of all SFVs described to date.…”

Section: Diversity and Origin Of Sfvs In The Americasmentioning

confidence: 99%

“…The long terminal repeat (LTR) region has a mean length of 1696-bp among OWP SFVs, with the longest LTR in SFVs infecting a pileated gibbon (SFVhpi), at 2074-bp [21,33], while an SFV infecting Cebidae has the shortest LTR among FVs (1061–1080-bp). SFVaxx has LTRs ranging from 1129-bp (SFVbar) to 1251-bp (SFVasp) in length [21,22]. Whether gene or LTR length differences among SFVs affect their biological functions remains to be elucidated.…”

Section: Diversity and Origin Of Sfvs In The Americasmentioning

confidence: 99%

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Simian Foamy Viruses in Central and South America: A New World of Discovery

Santos

Cavalcante

Muniz

et al. 2019

Viruses

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Foamy viruses (FVs) are the only exogenous retrovirus to date known to infect neotropical primates (NPs). In the last decade, an increasing number of strains have been completely or partially sequenced, and molecular evolution analyses have identified an ancient co-speciation with their hosts. In this review, the improvement of diagnostic techniques that allowed the determination of a more accurate prevalence of simian FVs (SFVs) in captive and free-living NPs is discussed. Determination of DNA viral load in American primates indicates that oral tissues are the viral replicative site and that buccal swab collection can be an alternative to diagnose SFV infection in NPs. Finally, the transmission potential of NP SFVs to primate workers in zoos and primate centers of the Americas is examined.

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Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics

Cosentino

D’arc

Moreira

et al. 2022

Preprint

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The development of high throughput sequencing (HTS) technologies and metagenomics protocols deeply impacted the discovery of viral diversity. Moreover, the comprehension of evolution and immunology of the Neotropical primates (NP) and their susceptibility to infectious diseases are central for the characterization of the scope of etiological agents that may impact global health, due to their evolutionary proximity to Old World primates, including humans. In the present work, novel anelloviruses were detected and characterized through HTS protocols in the NP Callithrix penicillata, the common black-tufted marmoset. De novo assembly of generated sequences was carried out and a total of 15 contigs were identified with complete Anelloviridae ORF1 gene, two of them including a flanking GC-rich region, confirming the presence of two whole novel genomes of ~3kb. The identified viruses were monophyletic within the Epsilontorquevirus genus, a lineage harboring previously reported anelloviruses infecting hosts from the Cebidae family. The genetic divergence found in the new viruses characterized two novel species, named Epsilontorquevirus callithrichensis I and II. The phylogenetic pattern inferred for the Epsilontorquevirus genus was consistent with the topology of their host species tree, echoing a virus-host diversification model observed in other viral groups. This study expands the host span of Anelloviridae and provides insights into their diversification dynamics, highlighting the importance of sampling animal viral genomes to obtain a clearer depiction of their long-term evolutionary processes.

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First Complete Genome Sequence of a Simian Foamy Virus Infecting the Neotropical Primate Brachyteles arachnoides

Cited by 6 publications

References 24 publications

Eco-Epidemiological Profile and Molecular Characterization of Simian Foamy Virus in a Recently-Captured Invasive Population of Leontopithecus chrysomelas (Golden-Headed Lion Tamarin) in Rio de Janeiro, Brazil

Eco-Epidemiological Profile and Molecular Characterization of Simian Foamy Virus in a Recently-Captured Invasive Population of Leontopithecus chrysomelas (Golden-Headed Lion Tamarin) in Rio de Janeiro, Brazil

Simian Foamy Viruses in Central and South America: A New World of Discovery

Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics

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