First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil

Costa, Vivaldo Gomes da; Saivish, Marielena Vogel; Oliveira, Priscila Gomes de; Silva-Júnior, Abelardo; Moreli, Marcos Lázaro; Krüger, Ricardo Henrique

doi:10.1038/s41598-021-92183-2

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…Another recent study presented complete genomic data which was acquired by Sanger sequencing method. These papers well represent the increasing need for rapid and specific genomic data generation 46 . Using the amplicon-based NGS sequencing technology is not unique in epidemic situations, but it was fairly used in veterinary health-related events to date.…”

Section: Discussionmentioning

confidence: 78%

Complete genomic sequencing of canine distemper virus with nanopore technology during an epizootic event

Lanszki

Tóth

Schütz³

et al. 2022

Sci Rep

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Canine distemper virus (CDV) endangers a wide range of wild animal populations, can cross species barriers and therefore representing a significant conservational and animal health risk around the globe. During spring to autumn 2021, according to our current estimates a minimum of 50 red foxes (Vulpes vulpes) died of CDV in Hungary, with CDV lesions. Oral, nasal and rectal swab samples were RT-PCR screened for Canine Distemper Virus from red fox carcasses. To investigate in more detail the origins of these CDV strains, 19 complete genomes were sequenced with a pan-genotype CDV-specific amplicon-based sequencing method developed by our laboratory and optimized for the Oxford Nanopore Technologies platform. Phylogenetic analysis of the complete genomic sequences and separately the hemagglutinin gene sequences revealed the role of the Europe lineage of CDV as a causative agent for the current epizootic. Here we highlight the growing importance of fast developing rapid sequencing technologies to aid rapid response activities during epidemics or epizootic events. We also emphasize the urgent need for improved surveillance of CDV, considering the epizootic capability of enzootic strains as reported in the current study. For such future efforts, we provide a novel NGS protocol to facilitate future genomic surveillance studies.

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Section: Discussionmentioning

confidence: 78%

Complete genomic sequencing of canine distemper virus with nanopore technology during an epizootic event

Lanszki

Tóth

Schütz³

et al. 2022

Sci Rep

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“…Next generation sequencing (NGS) technologies are increasingly being used to detect and characterize pathogens in wildlife [ 41 – 44 ]. MinION (Oxford Nanopore Technologies, Oxford, UK) has been used in many areas of virology, for instance, metagenomics or sequencing of complete genomes [ 45 – 48 ].…”

Section: Introductionmentioning

confidence: 99%

Detection and sequence analysis of Canine morbillivirus in multiple species of the Mustelidae family

Lanszki

Tóth

et al. 2022

BMC Vet Res

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Background Canine morbillivirus (canine distemper virus, CDV) is a member of the Paramyxoviridae family. Canine distemper is a serious viral disease that affects many mammalian species, including members of the Mustelidae family. These animals have an elusive nature, which makes related virological studies extremely challenging. There is a significant knowledge gap about the evolution of their viruses and about the possible effects of these viruses to the population dynamics of the host animals. Spleen and lung tissue samples of 170 road-killed mustelids belonging to six species were collected between 1997 and 2022 throughout Hungary and tested for CDV with real-time RT-PCR. Results Three species were positive for viral RNA, 2 out of 64 Steppe polecats (Mustela eversmanii), 1 out of 36 European polecats (Mustela putorius) and 2 out of 36 stone martens (Martes foina); all 18 pine martens (Martes martes), 10 least weasels (Mustela nivalis) and 6 stoats (Mustela erminea) tested negative. The complete CDV genome was sequenced in five samples using pan-genotype CDV-specific, amplicon-based Nanopore sequencing. Based on the phylogenetic analysis, all five viral sequences were grouped to the Europe/South America 1 lineage and the distribution of one sequence among trees indicated recombination of the Hemagglutinin gene. We verified the recombination with SimPlot analysis. Conclusions This paper provides the first CDV genome sequences from Steppe polecats and additional complete genomes from European polecats and stone martens. The infected specimens of various species originated from distinct parts of the country over a long time, indicating a wide circulation of CDV among mustelids throughout Hungary. Considering the high virulence of CDV and the presence of the virus in these animals, we highlight the importance of conservation efforts for wild mustelids. In addition, we emphasize the importance of full genomic data acquisition and analysis to better understand the evolution of the virus. Since CDV is prone to recombination, specific genomic segment analyses may provide less representative evolutionary traits than using complete genome sequences.

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“…The six structural proteins are nucleocaspid (N), phosphoprotein (P), matrix protein (M), fusion protein (F), hemagglutinin protein (H), and large protein (L). The two non-structural proteins, C and V, are encoded either by mRNA editing or by an alternate ORF in the gene that encodes P protein (da Costa et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Concomitant virus-induced gastrointestinal infection in dogs

Saltık

2023

Polish Journal of Veterinary Sciences

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Many viruses are involved in concomitant infections, which are prevalent in nature. In mixed infections, one or both infectious agents may be increased, reduced, or both may be increased while the other is suppressed. Canine distemper virus (CDV) and Canine parvovirus-2 (CPV-2) are important causes of gastroenteritis in dogs. Detection of these viruses is challenging since the symptoms are very similar. CDV is a member of the morbillivirus genus in the Paramyxoviridae family, and CPV-2 is a member of the Protoparvovirus genus in the Parvoviridae family; and both predominantly affect puppies and induce gastrointestinal symptoms in dogs. The purpose of this study was to contribute to the differential diagnosis of dogs with gastrointestinal symptoms. A PCR technique with specific primers was used to identify CDV and CPV-2 infections in gastroenteric dogs, and clinical changes in the infected dogs were monitored. The VP2 structural gene of CPV and the nucleocapsid gene of CDV were partially amplified in the study. PCR amplified the partial fragments of the CDV nucleocapsid (287 bp) and CPV-2 VP2 proteins (583 bp) from feces. In total, 3 out of 36 stool samples were positive for CDV and CPV-2 in the same dogs. Gasterointestinal symptoms also supported the diagnosis of concomitant infection with CDV and CPV-2 in these dogs. Dehydration and diarrhea in dogs can be signs of various diseases, such as viral, bacterial, and parasitic infections. After the elimination of non-viral pathogens, CDV and CPV-2 should also be simultaneously investigated to establish what is causing these symptoms. This study demonstrates the potential utility of correct diagnosis for the control of viral infection in dogs, but more research with a broader use of PCR-based detections is needed to assess its impact on differential diagnosis for concomitant infections.

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First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil

Cited by 6 publications

References 32 publications

Complete genomic sequencing of canine distemper virus with nanopore technology during an epizootic event

Complete genomic sequencing of canine distemper virus with nanopore technology during an epizootic event

Detection and sequence analysis of Canine morbillivirus in multiple species of the Mustelidae family

Concomitant virus-induced gastrointestinal infection in dogs

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