First comparative phenetic studies of the polymorphic species of Coccinella undecimpunctata Linnaeus, using morphometric and RAPD approaches in Egypt

Abstract

In this study, numerical phenetic method and RAPD-PCR technique were carried out on the species Coccinella undecimpunctata L. and its 13 aberrations which were collected from 10 localities in Egypt. The morphometric analysis and phenotypic features were determined for the aberrations to reveal the phenetic relationships among them. The resulted phenogram showed high degree of variations and affinities (Similarity coeffeciant ranged from 44.7 to 85.7%). RAPD fingerprint profiles were generated by using 5 random primers on genomic DNA to evaluate their phenetic relationships and to investigate the molecular markers among the aberrations genotypes. The similarity coefficient of the produced DNA fragments ranged from 40% to 93.75%. Cluster analysis based on both morphometric and RAPD data showed that the 14 morphs are grouped into 9 clusters against 8 clusters respectively. In addition, PCA plot allowed differentiating three groups from morphometric data against four groups from RAPD data.

First record of the introduced ladybird beetle, Coccinella undecimpunctata Linnaeus (1758), on South Georgia (sub‐Antarctic)

“…Specimens had small and elongate bodies (length ≈5 mm, Figure 2 ), 11 dark round spots on the red‐orange elytra, two distinct small white spots between the eyes and one white spot on each anterior margin of the pronotum, as described by Schaeffer ( 1912 ). The shape of the male reproductive system, in particular the sipho (Figure 2b ), corresponded with previous descriptions of C. undecimpunctata (Badrawy et al, 2009 ; Hawkes & Marriner, 1927 ). The species identity was established independently based on Figure 2 by two of the authors (HER and PT).…”

Taxonomical Revision of The Tribe Coccinellini (Coleoptera: Coccinellidae) In Egypt

“…The initial systematic assignment and identification of the included species and subspecies groups are based on a compromise between several compatible views on the systematics of Coccinellinae, Mulsant (1866), Beffa (1913), Mader (1930Mader ( -1931, Iablokoff-Khnzorian (1979), Al-Akkad (1979), Gordon & Vandenberg (1991), Raimundo et al (2000), Badrawy (2009), and Bedewy (2016), and the terminology of genitalia was based on Gordon (1985). The identified specimens were deposited in the side Collection of Plant Protection Department, Faculty of Agriculture, Al-Azhar University.…”

“…Many authors were studied and conducted this family, or some species, such as; Boehm his list of the Egyptian Coccinellidae in 1908; Ibrahim in 1953;Alfieri, 1976 in his list of "Coleoptera of Egypt"; (El-Akkad, 1979;Badrawy, 2009;Atif, 2016, andBedewy, 2016). This subfamily is represented in the Egyptian fauna by two tribes Coccinllini and Tyttaspidini= Bulaeaini (Bedewy, 2016).…”

Community Structure of Coccinellids in New Valley Governorate, Egypt

“…In Egypt, many authors are concerned with family Coccinellidae, or some species (Boehm, 1908;Ibrahim in 1953;El-Akkad, 1979;Badrawy, 2009;Atif, 2016, andBedewy, 2016;Abu El-Ghiet, 2019;El-Saeadyet al, 2020). These studies were fragment and scattered.…”