First Characterization in Tunisia of a TEM-15, Extended-Spectrum<i>β</i>-Lactamase-Producing<i>Klebsiella pneumoniae</i>Isolate

Chouchani, Chedly; Achour, Nahed Ben; M'Charek, Arij

doi:10.1089/mdr.2007.711

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…Among the 87 isolates, about twothirds produced ESBLs. Several studies have also shown the emerging problem of ESBL-producing E. coli and K. pneumoniae isolates in different geographic regions, including the Mediterranean basin [19] and North Africa [9,[20][21][22][23]. In our work, blaCTX-M-15 was the most frequently detected (54%) gene in the ESBLpositive Tunisian and Libyan isolates.…”

Section: Discussionsupporting

confidence: 55%

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Mathlouthi

Al-Bayssari

Salabi

et al. 2016

J Infect Dev Ctries

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Introduction: The aim of the study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among clinical isolates of Enterobacteriaceae recovered from Tunisian and Libyan hospitals. Methodology: Bacterial isolates were recovered from patients in intensive care units and identified by biochemical tests and MALDI-TOF. Antibiotic susceptibility testing was performed by disk diffusion and the E-test method. ESBL and carbapenemase activities were detected using standard microbiological tests. Antibiotic resistance-encoding genes were screened by PCR and sequencing. Clonal relationships between Klebsiella pneumoniae strains were carried out using multi-locus sequence typing (MLST). Results: A total of 87 isolates were characterized, with 51 and 36, respectively, identified as E. coli and K. pneumoniae. Overall the resistance prevalence was high for aminoglycosides (> 60%), fluoroquinolones (> 80%), and extended-spectrum cephalosporins (> 94%), and was low for imipenem (11.4%). Among this collection, 58 strains (66.6%) were ESBL producers and 10 K. pneumoniae strains (11.4%) were carbapenemase producers. The antibiotic resistance-encoding genes detected were blaCTX-M-15 (51.7%), blaTEM-1 (35.6%), several variants of blaSHV (21.8%), and blaOXA-48 (11.4%). The MLST typing of K. pneumoniae isolates revealed the presence of multiple clones and three novel sequence types. Also, close relationships between the OXA-48-producing strains from Tunisia and Libya were demonstrated. Conclusions: This study is the first paper describing the emergence of carbapenemase-and ESBL-producing Enterobacteriaceae, sensitive to colistin, isolated in Tunisia and Libya. Active surveillance and testing for susceptibility to colistin should be implementing because resistance to colistin, mainly in Klebsiella, has been recently reported worldwide.

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Section: Discussionsupporting

confidence: 55%

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Mathlouthi

Al-Bayssari

Salabi

et al. 2016

J Infect Dev Ctries

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“…The prevalence of ESBL and AmpC enzymes has increased worldwide [50,51,52]. However, the dissemination of ESBL and AmpC enzymes has also been reported in the neighboring country Tunisia [21,38,53,54,55]. In Saudi Arabia, Kader and his group (2005) also isolated ESBL-producing uropathogens from both inpatients and outpatients.…”

Section: Discussionmentioning

confidence: 99%

Emergence of AmpC β-Lactamase– and Metallo-β-Lactamase–producing Klebsiella pneumoniae in Sebha, Libya

Ahmad¹,

Alamen²,

Saad³

et al. 2019

Med J Islamic World Acad Sci

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Characterization of a Novel Extended-Spectrum TEM-Type β-Lactamase, TEM-164, in a Clinical Strain ofKlebsiella pneumoniaein Tunisia

Achour

Mercuri

Moussa³

et al. 2009

Microbial Drug Resistance

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Klebsiella pneumoniae ML1708 exhibited a multiresistance phenotype, including resistance to all beta-lactams tested, chloramphenicol, ciprofloxacin, nalidixic acid, tetracycline, and streptomycin. The double-disk synergy test was positive. ML1708 harbored a 50 kb conjugative plasmid that encoded a beta-lactamase of pI 5.5. The corresponding bla gene was identified by polymerase chain reaction and sequencing as a bla(TEM) gene. The deduced protein sequence revealed a new variant of TEM-1 beta-lactamase designated TEM-164. TEM-164 contains the unusual following mutations: L40V and I279T. These modifications may result in a change of the pI to 5.5 and hydrolyze cefotaxime and ceftazidime.

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First Characterization in Tunisia of a TEM-15, Extended-Spectrumβ-Lactamase-ProducingKlebsiella pneumoniaeIsolate

Cited by 4 publications

References 33 publications

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Emergence of AmpC β-Lactamase– and Metallo-β-Lactamase–producing Klebsiella pneumoniae in Sebha, Libya

Characterization of a Novel Extended-Spectrum TEM-Type β-Lactamase, TEM-164, in a Clinical Strain ofKlebsiella pneumoniaein Tunisia

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