First case series of emerging Rickettsial neonatal sepsis identified by polymerase chain reaction-based deoxyribonucleic acid sequencing

Aarthi, P; Bagyalakshmi, R; Mohan, KR; Manjunath, K; M, Nitin; Hn, Madhavan; Kalyani, S

doi:10.4103/0255-0857.118874

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…7 Timely diagnosis is important as easy, effective, and affordable treatment is available. 1,5 Newborn presents with symptoms mimicking sepsis like fever, respiratory distress, abdominal distension, poor feeding, lethargy, seizures, and hepatosplenomegaly. 8 The clues which made us think on parallel lines were a clear X-ray picture, hepatosplenomegaly, raised total lymphocyte counts with thrombocytopenia along with positive maternal history.…”

Section: Discussionmentioning

confidence: 99%

“…2 Transplacental infection is the vertical transmission from mother to fetus, in which IgM ELISA will be positive for the baby. 1 Perinatal blood-borne infection may not have IgM positivity. IgM antibodies titer can be detected after the first week of infection, while IgG antibodies appear at the end of the second week.…”

Section: Discussionmentioning

confidence: 99%

“…Scrub typhus, caused by Orientia tsutsugamushi , is the most common rickettsial infection in India. 1 The bite of the trombiculid mite larvae is the primary mode of transmission. Vertical transmission from mother to newborn was reported for the first time in 1992.…”

Section: Introductionmentioning

confidence: 99%

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Neonatal Scrub Typhus—A Case Report

Mehta

Choudhary

Bagri

et al. 2022

Journal of Neonatology

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Scrub typhus is the most common rickettsial infection in India. A neonate with suspected late-onset sepsis/late hemorrhagic disease of newborn and scrotal cellulitis was finally diagnosed as scrub typhus. The samples of blood and urine were sent and azithromycin was started empirically awaiting the reports. The baby improved with resolution of fever and increased oral acceptance and thrombocytopenia resolved. In any newborn presenting with fever, eschar, and positive family history, besides other common diagnoses like sepsis, scrub typhus should also be suspected as a differential diagnosis, especially in endemic countries. Prompt diagnosis and early initiation of treatment will reduce mortality and morbidity of newborns with scrub typhus.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Neonatal Scrub Typhus—A Case Report

Mehta

Choudhary

Bagri

et al. 2022

Journal of Neonatology

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“…No serosurvey reports on coxiellosis in Indian animals appeared after the eighties, until the recent report of coxiellosis in small ruminants of Puducherry in 2014 [32]. Evidence of animal and human abortions, neonatal septicemia, endocarditis, and atypical pneumonia due to C. burnetii based on immunofluroescence test/polymerase chain reaction (PCR) are recorded in recent Indian literature [4,5,33,34]. …”

Section: Introductionmentioning

confidence: 99%

Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Pradeep¹,

Spellman²,

Pratheesh³

et al. 2017

Vet World

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Background and Aim::In the course of our Indian Council of Medical Research project on coxiellosis in Puducherry and Tamil Nadu, 5.64% goat, 1.85% sheep, 1.06% buffaloes, and 0.97% cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland). In this preliminary study, we have proceeded to look for C. burnetii DNA in those antibody positive specimens employing an imported commercial C. burnetii polymerase chain reaction (PCR) kit.Materials and Methods::Blood samples were collected during slaughtering. All 15 blood samples of antibody positive ruminants and three antibody negative samples were subjected to conventional Trans-PCR assay with a commercial PCR kit (Genekam Biotechnology AG, Duisburg, Germany). An in-house Trans-PCR was included in the study for comparison.Results::A total of 15 antibody positive and three antibody-negative serum samples belonging to 11 goat, 4 sheep, 1 cattle, and 2 buffaloes were tested in duplicate for the presence of C. burnetii DNA by the commercial agar gel PCR kit and an in-house Trans-PCR. Only one buffalo serum sample was positive for C. burnetii with a band at 243 bp in in-house Trans-PCR.Discussion::Seropositivity for C. burnetii need not necessarily translate into infectivity status of the animal. Conversely, seronegative ruminants can shed C. burnetii. Rapid disintegration of C. burnetii DNA during the storage period is an important impediment in QF-PCR research. This is the first time the performance of this commercial PCR kit is being validated in India.Conclusion::Commercial PCR kit, Genekam did not identify any positive sample, probably because it targeted a larger amplicon of 687 bp.

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“…Endocarditis is the most common manifestation of chronic QF15. Diagnosis of QF is through serological tests such as the complement fixation test, enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay (IFA) and molecular tests for C. burnetii DNA6789101112131415. Coxiellosis in domestic animals of India has been reported by several researchers16171819.…”

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confidence: 99%

Detection of acute Q fever human cases by indirect immunofluorescence & real-time polymerase chain reaction in a tertiary care hospital in Puducherry

et al. 2018

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First case series of emerging Rickettsial neonatal sepsis identified by polymerase chain reaction-based deoxyribonucleic acid sequencing

Abstract: PCR-based DNA sequencing is a rapid and reliable diagnostic tool to identify the aetiological agents of neonatal sepsis. This is the first case series of emerging Rickettsial neonatal sepsis in India .

Cited by 17 publications

References 14 publications

Neonatal Scrub Typhus—A Case Report

Neonatal Scrub Typhus—A Case Report

Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Detection of acute Q fever human cases by indirect immunofluorescence & real-time polymerase chain reaction in a tertiary care hospital in Puducherry

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