First case of soft shell disease in Chinese soft-shelled turtle (Trionyx sinens) associated with Aeromonas sobria–A. veronii complex

Chen, Jianshun; Zhu, Ning; Kong, Lingfeng; Bei, Yi; Zheng, Taiying; Ding, Xueyan; He, Zhongyang

doi:10.1016/j.aquaculture.2013.05.006

Cited by 45 publications

(32 citation statements)

References 14 publications

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“…E. tarda and C. freundii have been isolated from clinical samples of soft-shelled turtles with fulminant septicaemia (Hu et al 2010). In agreement with previous studies (Kohler 2006;HernandezDivers et al 2009;Hu et al 2010;Chen et al 2013a;Chen et al 2013b), we identified aeromonads and enteric bacteria as the major pathogens associated with septicaemia and skin/shell diseases in softshelled turtles. However, to the best of our knowledge, there is no information regarding infection with W. chitiniclastica and Comamonas spp.…”

Section: Discussionsupporting

confidence: 80%

“…Aeromonas spp. have frequently been isolated from the liver or kidney of soft-shelled turtles with various clinical manifestations, such as soft shell and white abdominal shell (Chen et al 2013a;Chen et al 2013b). In addition, M. morganii and C. indologenes have been suggested to be infectious agents associated with shell necrosis in map turtles (Graptemys spp.…”

Section: Discussionmentioning

confidence: 99%

“…Veterinarni Medicina, 62, 2017(03): 169-177 doi: 10.17221/65/2016-VETMED et al 2010Chen et al 2013a;Chen et al 2013b). Chryseobacterium spp.…”

Section: Original Paperunclassified

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Identification and antibiotic resistance profiling of bacterial isolates from septicaemic soft-shelled turtles (Pelodiscus sinensis)

Chung¹,

Yi²,

Kim³

et al. 2017

Vet. Med.

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ABSTRACT:The present study sought to identify pathogens associated with septicaemia in the Chinese softshelled turtle (Pelodiscus sinensis) and to characterise antibiotic resistance in these pathogens. Twenty-three isolates recovered from the livers of diseased soft-shelled turtles were genetically identified as Aeromonas hydrophila (n = 8), A. veronii (n = 3), Citrobacter freundii (n = 4), Morganella morganii (n = 3), Edwardsiella tarda (n = 2), Wohlfahrtiimonas chitiniclastica (n = 1), Chryseobacterium sp. (n = 1), and Comamonas sp. (n = 1). Most isolates (n = 21) were resistant to ampicillin whereas a low percentage of isolates was susceptible to aminoglycosides (amikacin, gentamicin, and tobramycin). PCR assays and sequence analysis revealed the presence of the qnrS2 and bla TEM antibiotic resistance genes in all isolates. The bla DHA-1 , bla CTX-M-14 and bla CMY-2 genes were harboured by 17.4% (n = 4), 13.5% (n = 3) and 8.7% (n = 2) of the strains, respectively. One or more tetracycline resistance genes were detected in 60.9% (n = 14) of the isolates. Four isolates (17.4%) harboured single or multiple class 1 integron cassettes. Collectively, a variety of bacterial pathogens were involved in the occurrence of septicaemia in Chinese soft-shelled turtles and most of the isolates had multi-antibiotic resistant phenotypes. To our knowledge, the present report is the first to identify W. chitiniclastica and Comamonas sp. as causes of septicaemia in softshelled turtles and the first to identify Aeromonas spp. with bla and bla DHA-1 resistance genes.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Identification and antibiotic resistance profiling of bacterial isolates from septicaemic soft-shelled turtles (Pelodiscus sinensis)

Chung¹,

Yi²,

Kim³

et al. 2017

Vet. Med.

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“…It is necessary to identify and classify different species within the genus (Janda & Abbott ). Biochemical characterization and 16S rRNA sequence analysis both showed limited uses for differentiation of Aeronomas isolates within close species (Chen, Zhu, Kong, Bei, Zheng, Ding & He ). Subsequent subtyping methods are required for further partitioning of Aeromonas isolates.…”

mentioning

confidence: 99%

Prevalence and antimicrobial susceptibility ofAeromonasspecies from diseased Chinese soft-shelled turtles (Trionyx sinens)

Chen¹,

Ding²,

Zhu³

et al. 2013

Aquac Res

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“…Aeromonas and Pseudomonas spp. have been isolated from turtles with pneumonia, and many other infections in turtles with bacterial aetiologies have been described (McArthur 2004;Kohler 2006;Hernandez-Divers et al 2009;Chen et al 2013;Chung et al 2017).…”

mentioning

confidence: 99%

Comparative in vitro efficacy of eight essential oils as antibacterial agents against pathogenic bacteria isolated from pet-turtles

Silva¹,

Hossain²,

Wimalasena³

et al. 2018

Vet. Med. - Czech

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Essential oils are plant extracts that have been used for their antimicrobial properties for centuries. The keeping of turtles as pets exhibits a growing trend worldwide but these animals are known to harbour a range of pathogenic bacteria. In the current study, we assessed eight essential oils as alternative antibacterial agents against nine species of pet turtle-borne Gram-negative bacteria, namely Aeromonas caviae, A. dhakensis, A. hydrophila, Citrobacter freundii, Morganella morganii, Proteus mirabilis, P. vulgaris, Pseudomonas aeruginosa and Salmonella enterica. Except for Pseudomonas aeruginosa, all other bacterial species showed high susceptibility to six essential oils, namely oregano, cinnamon, clove, lemongrass, lavender and eucalyptus oils in descending order of efficacy. Minimum inhibitory concentrations and minimum bactericidal concentrations values of the essential oils against all tested species except for P. aeruginosa showed low heterogeneity, showing that these essential oils can effectively control the growth of nearly all the tested. However, most of the tested bacteria were multiple-antibiotic-resistant as determined in the antibiotic disc diffusion test, with multiple-antibiotic-resistant index values of ≥ 0.2 for most of the strains. Therefore, with regards to their in vitro activity in controlling growth of multi-drug resistant bacteria, we can classify oregano, cinnamon, clove, lemongrass, lavender and eucalyptus essential oils as effective antibacterial agents. Thus, prospective application of these essential oils in controlling and treating these bacteria should be considered.

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First case of soft shell disease in Chinese soft-shelled turtle (Trionyx sinens) associated with Aeromonas sobria–A. veronii complex

Cited by 45 publications

References 14 publications

Identification and antibiotic resistance profiling of bacterial isolates from septicaemic soft-shelled turtles (Pelodiscus sinensis)

Identification and antibiotic resistance profiling of bacterial isolates from septicaemic soft-shelled turtles (Pelodiscus sinensis)

Prevalence and antimicrobial susceptibility ofAeromonasspecies from diseased Chinese soft-shelled turtles (Trionyx sinens)

Comparative in vitro efficacy of eight essential oils as antibacterial agents against pathogenic bacteria isolated from pet-turtles

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