First autochtonous infection of a dog with Oslerus (Filaroides) osleri in the Czech Republic

Abstract

This case report describes an infection with O. osleri in a 10-month-old intact female Miniature German Spitz that presented with a 3-month history of progressive cough. Diagnosis was based upon visualization of characteristic lesions during bronchoscopy. Female parasites and first-stage larvae collected from tracheal nodules were morphologically identical to the larvae of O. osleri. First-stage larvae isolated from faeces were used for morphological and molecular confirmation of the diagnosis. Anthelmintic therapy with fenbendazole (50 mg/kg orally once daily for 2 weeks) was successful. This is the first report of autochthonous infection of a dog with O. osleriin the Czech Republic. Oslerosis should be considered in the differential diagnosis in young dogs with persistent respiratory signs.

Morphological and molecular evidence of Oslerus osleri (Nematoda: Filaroididae) in the Andean fox (Lycalopex culpaeus)

“…However, O. osleri diagnosis was confirmed by molecular analysis. The nucleotide sequences of ribosomal and mitochondrial genes from this study were more than 98% similar to sequences referenced from GenBank (Accession numbers: JQ730005, JX185314, and AY292800) (Carreno and Nadler, 2003;Husnik et al, 2016;Verocai et al, 2013). Studies have demonstrated that nematode body size can change by the action of their nutrients and microbial food parameters (So et al, 2012).…”

“…Currently, there are few molecular studies performed in O. osleri, which have analyzed various genetic markers that include ribosomal genes (large and small subunit) and the ITS-2 region (Carreno and Nadler, 2003;Husnik et al, 2016;Verocai et al, 2013). In this study, we amplified partial cox1, partial LSU rRNA, and the full ITS-2 region.…”

Complete resolution of diffuse inflammatory tracheal stenosis in a dog

“…The use of oral colchicine has also been described in the management of refractory cases 16 17. Parasitic infections of the trachea, despite arising infrequently, also seem to cause granulomatous inflammation 3–5. The most common tracheal parasite is O osleri and the adult worms are typically found in nodules at the carina and can be easily identified on tracheoscopy, while larvae can be detected using faecal Baermann or zinc sulphate flotation examination 3 5.…”

“…Parasitic infections of the trachea, despite arising infrequently, also seem to cause granulomatous inflammation 3–5. The most common tracheal parasite is O osleri and the adult worms are typically found in nodules at the carina and can be easily identified on tracheoscopy, while larvae can be detected using faecal Baermann or zinc sulphate flotation examination 3 5. A single reported case of granulomatous inflammation of the trachea involving Onchocerca species has also been described, and this parasite should also be considered as a differential diagnosis in dogs with nodular tracheal lesions 4.…”

“…Possible differential diagnoses for intraluminal tracheal nodules in dogs included parasites ( Oslerus osleri , Onchocerca species),3–5 primary and metastatic neoplasia (cartilaginous tumours such as osteochondroma, chondroma, chondrosarcoma; mast cell tumour, leiomyoma),6 7 eosinophilic granuloma,8 nodular hyperplastic tracheitis,9 nodular amyloidosis,10 tracheal papillomatosis11 and tracheobronchopathia osteochondroplastica 12…”