First 10 000 chorionic villus samplings performed on singleton pregnancies by a single operator

Abstract

Chorionic villus sampling (CVS) was performed in 10 000 consecutive singleton pregnancies by a single principal operator, working in two institutions. The procedure was performed between 8 and 32 gestational weeks: transabdominal (TA) sampling was carried out in 8479 cases and transcervical (TC) in 1521. Patients were referred for chromosomal risk in 89·1 per cent of cases, Mendelian disorders in 10·5 per cent, and DNA investigations for paternity or infectious agents in 0·4 per cent of cases. The sampling success rate for both TA and TC techniques by the second insertion was 99·8 and 99·2 per cent, respectively. TA sampling succeeded in a higher number of cases at the first insertion (98 per cent vs. 86·8 per cent) and was associated with smaller samples (<10 mg) in fewer cases (3·2 per cent vs. 4·9 per cent). Cytogenetic analysis was highly successful (99·4 per cent) and accurate; however, in one case a de novo structural rearrangement of chromosome 1 was not recognized. Mosaicism or rare trisomies were reported in 1·30 per cent of cases. Five diagnostic errors in DNA investigation (0·51 per cent) ended with the birth of affected fetuses. Fetal loss through 28 weeks' gestation in the pregnancies intended to continue was 2·58 per cent; the rate increased with maternal age (1·22 per cent at less than 30 years to 3·8 per cent at 40 years or more), while gestational age affected the abortion rate only at 8 weeks (odds ratio=2·22, P<0·05). Rates of premature delivery, low birth weight, and perinatal mortality did not differ from the Italian standards. By comparison with the Italian Birth Defects Registry data, no differences were found for the major malformations, including transverse limb reduction defects (TLRDs) (4·34 vs. 3·28×10 000). Total malformations and TLRDs did not show any pattern relation to either maternal age or gestational age. © 1998 John Wiley & Sons, Ltd.

Summary of 2185 prenatal invasive procedures in a single center: A retrospective analysis

“…Another study in a Turkish population focused on the same issue and revealed a CA rate of 4.4% ( 12 ) . The medical literature reports culture media failure lower than 1.0% ( 11 , 13 , 14 , 15 ) and we observed approximately the same rate (1.2%). The patients and their spouses accepted the second attempt in only 7 of 27 cases (6 CVS and one AC) and a new AC was performed, all of which revealed a normal karyotype.…”

“…The rates of other indications were also compatible with the literature ( 8 , 9 ) . Our study demonstrated a CA rate up to 7% (154/2185) ( 7 , 10 , 11 ) . Another study in a Turkish population focused on the same issue and revealed a CA rate of 4.4% ( 12 ) .…”

Zur Komplikationsrate bei invasiven, intrauterinen Eingriffen an einer pränataldiagnostischen Schwerpunktabteilung

“…Diese Daten decken sich annähernd mit den Ergebnissen einer australischen Multizenterstudie (1,2 % Abortusrate) [11] sowie den Angaben von Brambati et al [12], der 10 000 Chorionzottenbiopsien, die von einem einzigen Operateur vorgenommen wurden, retrospektiv aufgearbeitet hat (Abortusrate 1,2 -3,8 %).…”