FIR-preconditioning promotes Akt-mTOR-exosome manufacture in cooperation with MITF to boost resilience of rat bone marrow-derived stem cells

Jeong, Yun-Mi; Kim, Weon

doi:10.1016/j.heliyon.2023.e15003

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The A375 and CCD-986 SK cells were cultured in complete medium [DMEM-high glucose supplemented with 10% FBS and 1% PS (penicillin-streptomycin)]. To assess the dose-dependent effects of LMW-F on A375 cell viability, cells were treated with LMW-F at concentrations ranging from 0, 1, 5, 10, 20, 50 μg/mL and incubated for 24 h. Cell viability was then assessed using a live/dead assay and MTT assay, as described previously [ 13 – 15 ]. 1–2 mm PDME fragments were obtained from patient-derived melanoma xenograft models, which were sourced from Seoul St. Mary’s Hospital (Seoul, Korea), as described in our prior studies [ 13 – 15 ].…”

Section: Methodsmentioning

confidence: 99%

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Low-molecular-weight fucoidan inhibits the proliferation of melanoma via Bcl-2 phosphorylation and PTEN/AKT pathway

PARK,

BANG,

YUN

et al. 2024

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Fucoidan, a sulfate polysaccharide obtained from brown seaweed, has various bioactive properties, including anti-inflammatory, anti-cancer, anti-viral, anti-oxidant, anti-coagulant, anti-thrombotic, anti-angiogenic, and anti- Helicobacter pylori properties. However, the effects of low-molecular-weight fucoidan (LMW-F) on melanoma cell lines and three dimensional (3D) cell culture models are not well understood. This study aimed to investigate the effects of LMW-F on A375 human melanoma cells and cryopreserved biospecimens derived from patients with advanced melanoma. Ultrasonic wave was used to fragment fucoidan derived from Fucus vesiculosus into smaller LMW-F. MTT and live/dead assays showed that LMW-F inhibited cell proliferation in both A375 cells and patient-derived melanoma explants in a 3D-printed collagen scaffold. The PTEN/AKT pathway was found to be involved in the anti-melanoma effects of fucoidan. Western blot analysis revealed that LMW-F reduced the phosphorylation of Bcl-2 at Thr 56, which was associated with the prevention of anti-apoptotic activity of cancer cells. Our findings suggested that LMW-F could enhance anti-melanoma chemotherapy and improve the outcomes of patients with melanoma resistance.

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Section: Methodsmentioning

confidence: 99%

“…Cell viability and proliferation assays were conducted using a live and dead assay and MTT assay, as previously described [ 13 – 15 ]. Absorbance of MTT assay was measured using a spectrometer (Emax; Molecular Devices, Sunnyvale, CA, USA).…”

Section: Methodsmentioning

confidence: 99%