Fir honeydew honey flavonoids inhibit TNF-α-induced MMP-9 expression in human keratinocytes: a new action of honey in wound healing

Abstract

Matrix metalloproteinase-9 (MMP-9) appears to be a major protease responsible for the degradation of matrix and growth-promoting agents in chronic wounds. Honey has been successfully used for treating non-healing wounds associated with infections. However, the mechanisms of its action at the cellular level have remained poorly understood. The aim of this study was to investigate the effect of fir honeydew honey on TNF-α-induced MMP-9 expression and secretion from human keratinocytes (HaCaT) and to identify the honey component(s) responsible for a discovered effect. A C18 solid-phase column was used for preparation of honey aqueous extract (HAE). Expression and production of MMP-9 by HaCaT cells were determined by reverse transcription-PCR, gelatine zymography and Western blot analysis using a polyclonal antibody against MMP-9. We found that HAE inhibited TNF-α-induced production of MMP-9 in keratinocytes in a dose-dependent manner at both the mRNA and protein levels. Apigenin and kaempferol, identified flavonoids in HAE, markedly inhibited MMP-9 production from HaCaT and epidermal keratinocytes. Taken together, fir honeydew honey, which contains certain flavonoids, prevents TNF-α-induced proteolytic activity in cutaneous inflammation. Thus, our findings provide clear evidence that honey may serve as a natural treatment for dermatological problems associated with a persistent inflammation.

Avaliação das atividades cicatrizante, anti-inflamatória tópica e antioxidante do extrato etanólico da Sideroxylon obtusifolium (quixabeira)

“…Além disso, os flavonoides possuem capacidade de inibir a desgranulação de neutrófilos e reduzir a ativação do complemento, diminuindo assim a adesão de células inflamatórias ao endotélio e a resposta inflamatória (Nijveldt et al, 2001). Os flavonoides também inibem a produção de metaloproteinase-9 em queratinócitos humano durante o processo de cicatrização de feridas (Majtan et al, 2013). Taninos, também presentes no EE da S. obtusifolium, são capazes de inibir as enzimas COX-2 e/ou iNOS (Carvalho, 2004).…”

Honey: an immunomodulatory agent for disorders of the skin

“…Contrary to the immunostimulatory effects of honey described above Majtan et al (2013) reported an anti-inflammatory effect of an aqueous extract of fir honeydew honey obtained from an apiary in Bardejov, Slovakia. In this study, the extract inhibited TNFα induced matrix metalloproteinase-9 (MMP-9) protein and mRNA production by human keratinocytes (HaCaT) cells as measured by ELISA and RT-PCR respectively.…”

“…More recently, in vitro research has suggested that honeys of diverse floral origins can modulate the skin immune system; both immunostimulatory and antiinflammatory effects have been observed (Majtan, Kumar, Majtan, Walls & Klaudiny, 2009;Majtan et al, 2013). In this review, the scientific evidence for the immunomodulatory properties of honey in relation to disorders of the skin is evaluated and the mechanisms of the immunomodulatory properties explored.…”

“…Honey or its components have been shown to modulate cytokine production by a range of cells (Majtan et al, 2013;Tonks et al, 2003). It may be that honey contributes to skin healing not only through its anti-microbial properties but by boosting the skin immune system to fight infection, promoting wound healing or suppressing inflammation in the skin.…”

Burn Wound Healing Effect and Hair Growth Promoting Activity of <i>Lawsonia inermis</i> L. and Honey in <i>Oryctolagus cuniculus</i> Rabbits

“…It is naturalized and cultivated in the tropics of America, Egypt, India and parts of the Middle East (Muhammad and Muhammad, 2005). The different parts of henna have been reported to exert antioxidant, hypoglycemic, antidiabetic, hepatoprotective, antibacterial, antifungal, anticancer and wound healing properties (Majtan et al, 2013;Hadagali and Chua, 2014;Rajashri and Sachin, 2014;El Bergadi et al, 2015;Devasvaran and Yong, 2016;Mohamed et al, 2016).…”