Finite element model for nutrient distribution analysis of a hollow fiber membrane bioreactor

Unnikrishnan, Ginu; Unnikrishnan, Vinu; Reddy, J. N.

doi:10.1002/cnm.1462

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…Figure 12 Normalized oxygen concentration distribution in the scaffold from the finite element simulations for different cell densities [49]. Table 2 Material properties for convective bioreactor analysis [49].…”

Section: Fluid Flow and Distribution Of Oxygen Within The Hfmbmentioning

confidence: 99%

“…As the permeability of the Table 1 Material properties for diffusive bioreactor analysis [49]. Figure 12 Normalized oxygen concentration distribution in the scaffold from the finite element simulations for different cell densities [49].…”

Section: Fluid Flow and Distribution Of Oxygen Within The Hfmbmentioning

confidence: 99%

“…The difference in the predicted and experimentally obtained values could be due to the microstructural formation during electrospinning, which could not be accounted in the mathematical homogenization models. The effective property of the scaffold is now estimated using the 3D open cell model [11,49] and the DM estimates. On the basis of the homogenization studies from the previous sections, an average modulus of 10.9 GPa for the nanofiber is considered.…”

Section: Dewey-mackenzie Estimatesmentioning

confidence: 99%

“…Variation of elastic modulus of nanotube-reinforced PVA scaffold with void volume fractions[49]. large number of variables and the difficulty in obtaining real-time data make experimental studies tedious.…”

mentioning

confidence: 99%

“…Schematic representation of fully developed flow in porous/fluid domain[49]. Velocity profile comparison between finite element simulation and analytical solution[49].…”

mentioning

confidence: 99%

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Recent advances in the analysis of nanotube-reinforced polymeric biomaterials

Reddy

Unnikrishnan

2013

Journal of the Mechanical Behavior of Materials

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Conventional experimental or computational techniques are often inadequate for the analysis and development of nanocomposite-based materials as they are tedious (e.g., experimental methods) or are unsuitable to capture the properties of these novel materials (e.g., conventional computational techniques), thereby requiring multiscale computational strategies. During the last 5 years, major developments were made by the authors on the formulation and implementation of multiscale computational models, using atomistic simulation and micromechanics-based techniques, to study the mechanical and thermal behavior of nanocomposite-based materials. In this article, the advances made in the computational analysis of nanocomposites for tissue engineering applications (e.g., scaffolds and bioreactors) would be discussed. The material properties of the nanocomposites in the lower scales were determined using molecular dynamics, and were then transferred to the macroscale using various homogenization techniques. Also in this article, the authors discuss the development of a theory of mixturebased finite element model for nutrient flow in a hollow fiber membrane bioreactor and the use of computational tools to improve the efficiency of the bioreactor.

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Section: Fluid Flow and Distribution Of Oxygen Within The Hfmbmentioning

confidence: 99%

Section: Fluid Flow and Distribution Of Oxygen Within The Hfmbmentioning

confidence: 99%

Section: Dewey-mackenzie Estimatesmentioning

confidence: 99%

mentioning

confidence: 99%

“…Schematic representation of fully developed flow in porous/fluid domain[49]. Velocity profile comparison between finite element simulation and analytical solution[49].…”

mentioning

confidence: 99%

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Recent advances in the analysis of nanotube-reinforced polymeric biomaterials

Reddy

Unnikrishnan

2013

Journal of the Mechanical Behavior of Materials

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Computational Modelling of Tissue-Engineered Cartilage Constructs

Bandeiras

Completo

2020

Lecture Notes in Computational Vision and Biomechanics

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Cartilage is a fundamental tissue to ensure proper motion between bones and damping of mechanical loads. This tissue often suffers damage and has limited healing capacity due to its avascularity. In order to replace surgery and replacement of joints by metal implants, tissue engineered cartilage is seen as an attractive alternative. These tissues are obtained by seeding chondrocytes or mesench ymal stem cells in scaffolds and are given certain stimuli to improve establishment of mechanical properties similar to the native cartilage. However, tissues with ideal mechanical properties were not obtained yet. Computational models of tissue engineered cartilage growth and remodelling are invaluable to interpret and predict the effects of experimental designs. The current model contribution in the field will be presented in this chapter, with a focus on the response to mechanical stimulation, and the de velopment of fully coupled modelling approaches incorporating simultaneously solute transport and uptake, cell growth, production of extracellular matrix and remodelling of mechanical properties.

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Relative Effects of Fluid Oscillations and Nutrient Transport in the In Vitro Growth of Valvular Tissues

Salinas

Rath

Villegas

et al. 2016

Cardiovasc Eng Tech

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Engineered valvular tissues are cultured dynamically, and involve specimen movement. We previously demonstrated that oscillatory shear stresses (OSS) under combined steady flow and specimen cyclic flexure (flex-flow) promote tissue formation. However, localized efficiency of specimen mass transport is also important in the context of cell viability within the growing tissues. Here, we investigated the delivery of two essential species for cell survival, glucose and oxygen, to 3-dimensional (3D) engineered valvular tissues. We applied a convective-diffusive model to characterize glucose and oxygen mass transport with and without valve-like specimen flexural movement. We found the mass transport effects for glucose and oxygen to be negligible for scaffold porosities typically present during in vitro experiments and non-essential unless the porosity was unusually low (<40%). For more typical scaffold porosities (75%) however, we found negligible variation in the specimen mass fraction of glucose and oxygen in both non-moving and moving constructs (p > 0.05). Based on this result, we conducted an experiment using bone marrow stem cell (BMSC)-seeded scaffolds under Pulsatile flow-alone states to permit OSS without any specimen movement. BMSC-seeded specimen collagen from the pulsatile flow and flex-flow environments were subsequently found to be comparable (p > 0.05) and exhibited some gene expression similarities. We conclude that a critical magnitude of fluid-induced, OSS created by either pulsatile flow or flex-flow conditions, particularly when the oscillations are physiologically-relevant, is the direct, principal stimulus that promotes engineered valvular tissues and its phenotype, whereas mass transport benefits derived from specimen movement are minimal.

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Finite element model for nutrient distribution analysis of a hollow fiber membrane bioreactor

Cited by 6 publications

References 26 publications

Recent advances in the analysis of nanotube-reinforced polymeric biomaterials

Recent advances in the analysis of nanotube-reinforced polymeric biomaterials

Computational Modelling of Tissue-Engineered Cartilage Constructs

Relative Effects of Fluid Oscillations and Nutrient Transport in the In Vitro Growth of Valvular Tissues

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